... hese conditions, by t hepresence of a protein band with a slower mobility (lane 3, *)occurring within the ascomplex. As the as1-Cas and as2-Casoverlap in the same zone of the gel, the ... as1-CasA and Bvariants, must be considered a ‘strong’ variant, given the intensity of the isoelectrofocusing bands and the surface of the relevant peak in RP-HPLC.Fig. 6. Nucleotide sequence of the ... both the protein and genomic level. The completeamino-acid sequence of the M variant has been determined.Starting from genomic DNA, we amplified, by PCR, the coding regions (exons) a nd their intron...