Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... 2002 Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile Eva Czerwiec1, Gail S. Begley1, Mila Bronstein2, Johan Stenflo1,3, ... the Conus carboxylase and vertebrate vitamin K-dependent c-carboxylasesargue for conservation of a vitamin K-dependent carb-oxylase across animal species and the importance of c-carboxyglutamic ... vita-min K-dependent carboxylase activity and vitamin K-dependent epoxidase activity. The recombinant carboxylase hasamolecularmassof 130 kDa. The recombinant Conus carboxylase carboxylated Phe-Leu-Glu-Glu-Leu...
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Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt
... theaddition of poly(I)Æpoly(C) only managed to doubleABFig. 1. SDS ⁄ PAGE (A) and western blot analysis (B) of the affinitypurified C-terminally and N-terminally His-tagged recombinant 2-5Asynthetase ... nuclease and ⁄ or poly(I)Æpoly(C)on the 2-5A synthesizing activity of the recombinant porcine 2-5Asynthetase (A) and N-terminally His-tagged recombinant 2-5A syn-thetase from G. cydonium (B). ... 2-5Asynthetase, and, second, the recombinant protein spon-taneously forms enzymatically active complexes withheterologous RNA. Characterization of the preparation by native gelanalysis and by...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... refolding and purification they were characterized by both structural and enzyme kinetic studies. Mutation of Tyr183 t o phenylalanineproduced an enzym e with a lower kcat and a slightly higherKmthan ... cause of these differences is not known.Km and kcatmeasurements of hevamine and mutantsComparison of the steady-state kinetic parameters of hevamine and the Tyr183Phe and Asp125Asn mutantsshows ... Asp125Alamutant still has 2% activity (Table 3).CONCLUSIONSIn this study we investigated the catalytic role of Asp125,Glu127 and Tyr183 in hevamine by X-ray crystallographic and kinetic analysis of...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... connectingstrand b5 and helix a3, while His290 and Asp260 e merge from the b8-a8andb7-a7 loops, respectively. Anotherimportant feature of the catalytic machinery is the so-calledoxyanion hole necessary ... (50%) and have biochemically definedRv1399c a s a non lipolytic hydrolase. This enzym eefficiently hydrolyzes short-chain synthetic substrates suchas triacylglycerols and vinyl esters and the ... Trp222Trp92Trp92Gly91Gly91Gly90Gly90Gly89Gly89Gly291Gly291T y r292 T y r292Tyr190 Tyr190Tyr190Tyr190His290His290His88His88Ser162Ser162Rv1399c Ag85cFusarium solani pisi cutinase...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... S.G. (1994) Cloning and sequencing of thiol-speci®cantioxidant from m ammalian brain: alkyl reductases and thiol-speci®c antioxidant de®ne a large family o f antioxidant enzymes.Proc. N atl ... by HPLC and some of themwere totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ( Fig. 2).Computer database searches based on the amino-acidsequences of ... reductase system in yeast and animals, w hile 1Cys-Prx may be r educed by a smallthiol molecule such as glutathione. R ecently, 1Cys-Prx hasbeen identi®ed i n y east and Arabi dopsis, and has...
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
... forpen-3a transcripts and 18 S rRNA probes were quantifiedbySTORM and analysed separately. Data analysis revealed an important individual variation in both pen-3a transcripts and 18 S rRNA ... constituted only 5% of the haemocytes analysed (Fig. 6A and B1). At 6, 12 and 48 h post-stimulation, this percentage increased, respect-ively, to 19, 23 and 34% of haemocytes displaying an AUvalue ... electron densegranules of two subtypes of infiltrating hae-mocytes, namely large-granule haemocytes(D, E) and small-granule haemocytes (F, G).No labelling was seen throughout cytoplasm and nucleus....
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Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
... transient contractionby activating ryanodine receptors of the sarcoplasmicFig. 1. Isolation of tigrin from the Duvernoy’s glands of R. tigrinustigrinus. (A) The extract from Duvernoy’s glands ... nonreducing (lane 1–4) and reducing (lane 5–8) conditions. The positions of molecular massmarkers are shown on the left. Lanes 1 and 5 show ablomin (26 and 29.7 kDa), lanes 2 and 6 show triflin (23 and ... Kumamoto, Japan) [38]. Other snake venoms and venom glands were purchased from the Japan SnakeInstitute (Gunma, Japan). Superdex 75 pg and 200 pg,SP–Sepharose High Performance, and Q-Sepharose...
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Báo cáo khoa học: Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot
... Mg2+may contribute to theweak binding of Mg2+ and enzymatic activity becauseproteins and enzymes generally bind Mg2+weakly[36]. Nevertheless, understanding the differences inbinding and enzymatic ... appli-cation of this type of enzymes. Further studies aimingto elucidate the physiological and structural character-istics of PepD and related M20 family enzymes and probe the discrepancies of different ... and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticusTing-Yi Wang*, Yi-Chin Chen*, Liang-Wei Kao, Chin-Yuan Chang, Yu-Kuo Wang, Yen-Hsi Liu,Jen-Min Feng and Tung-Kung...
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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx
... Zhang2 and Tadashi Yoshida21Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan;2Department of Biochemistry,Yamagata University School of Medicine, Japan An ... groups of chlorophyll and phycobilin.Chlorophyll, which is contained in all plants includingcyanobacteria and protoflorideophyceae, is synthesized from protoporphyrin IX, a precursor of heme. Phycobilins of ... enhanced and new peaksTable 2. g-Values and g-anisotropy of alkaline forms of heme–heme oxygenase complexes. Data for Hmu O and rat HO-1 are taken from refs [24] and [14], respectively. g-Anisotropy...
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Báo cáo Y học: Identification and characterization of thioredoxin and thioredoxin reductase fromAeropyrum pernixK1 pdf
... Identification and characterization of thioredoxin and thioredoxinreductase from Aeropyrum pernixK1Sung-Jong Jeon and Kazuhiko IshikawaNational Institute of Advanced Industrial Science and Technology ... selenite,selenoglutathione, vitamin K and alloxan [9,19]. In additionto TR having been isolated and characterized from a widevariety of bacterial and eukaryotic species, it has also beenfound in anaerobic hyperthermophilic ... properties of the recombinant proteinsThe proteins from APE0641 and APE1061 were expressedin E. coli cells, and the recombinant proteins were purifiedto homogeneity. Expression and subsequent...
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