Báo cáo khoa học: Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state ppt

Báo cáo khoa học: Reﬁned solution structure and backbone dynamics of the archaeal MC1 protein ppt

... role in the stabilization of the proteinÆDNA complex. The arm of MC1 can alsobe compared with the DNA-binding b-hairpin arms of HUs, which showed high mobility relative to the core(Fig. 2). The ... structure of methanogen chromosomal protein 1 (MC1), deter-mined with heteronuclear NMR methods, agrees with its function in terms of the shape and nature of the binding surface, whereas the 3D ... signiﬁcantincrease in 15N-NOE upon DNA binding are in blue, and those with an intermediateincrease are in marine. (B) Residues thatexhibit signiﬁcant CSP upon DNA bindingare in red, and those with...

Báo cáo khoa học: Sirt1 and mir-9 expression is regulated during glucose-stimulated insulin secretion in pancreatic b-islets ppt

... in insulin-secreting b-cells. In order to gainmore insight into the physiological role of mir-9 in insulin secretion, we have speciﬁcally addressed the link between GSIS and the regulation of ... altered in insulin-secreting b-isletsduring GSIS in vivo. Using b-TC-6 cells and conditionsthat mimic the declining phase of insulin secretion, weclearly demonstrate that mir-9 is involved in the ... have also examined the role of Sirt1 in cytokine-dependent b-cell cytotoxicity [24]. Interest-ingly, the data show that interferon-c and interleukin-1btreatment of insulin-secreting cells leads...

Báo cáo khoa học: Fowlicidin-3 is an a-helical cationic host defense peptide with potent antibacterial and lipopolysaccharideneutralizing activities ppt

... conserved glycine (Fig. 3E), it willbe important to examine the impact of further enhan-cing its ﬂexibility on the functional properties. In fact, the ﬂexibility of the hinge region has often been ... Ribbon diagram of the minimized average structure of fowlicidin-3. (D) Superimposition of the average structures of fowlicidin-3 with fowlicidin-1. The struc-tures were generated by usingMOLMOL. ... kink induced by a glycine at position 17. It is highlypotent against a broad range of Gram-negative and Gram-positive bacteria in vitro, including antibiotic-resistant strains, with minimum inhibitory...

Báo cáo khoa học: Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding pptx

... demonstratedthat the inhibition of PAI-1 on insulin signaling is inde-pendent of uPAR-VN binding. These results constitute the ﬁrst demonstration of the interaction of PAI-1 with the insulin response.Keywords: ... suggestingthat the VN enhancement of insulin signaling is dependenton avb3 integrin.To assess the potential role of PAI-1 in the VN-mediated induction of insulin signaling, PAI-1 wasincluded in ... role in the inhibition of insulin/VN signaling by PAI-1.To further assess the functional relevance of PAI-1/VN interaction on insulin signaling, we analyzed the expres-sion of an insulin-inducible...

Báo cáo khoa học: Salt-inducible kinase-1 represses cAMP response element-binding protein activity both in the nucleus and in the cytoplasm pdf

... in the nucleus. In any case, in the hope of determining domain(s) important for the intracellularlocalization, we ﬁrst attempted to investigate the intracel-lular distribution of a number of ... in bold. The basic residues in domain 3B areindicated by underlines. The similaritybetween SIK1 and SIK2 in domain 3A, the N-terminal half of domain 3, is higher thanthat in domain 3B, the C-terminal ... Notably, ﬁve of nine basic residues present in the R K-rich region arereplaced with other residues in the corresponding region of SIK2. To examine whether or not the lower similarity in domain 3 B...

Báo cáo khoa học: Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state ppt

... 1896–1905 ª 2006 The Authors Journal compilation ª 2006 FEBS 1905 Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state Gyo¨rgy ... producean increase in rotational motion in the environment of the Cys374 site of actin [8]. In a recent publication the authors argue that in the weak binding state of myosin to actin the heads interact ... Nevertheless, cross-linking actinmolecules impair the motor function of actomyosin.This effect may correspond to the above differences in the motion of the EPR probe in the weak-binding state of...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... twice in PBSCM supplied with 100 mm glycine, andincubated for 20 min to quench further cross-linking. The quenching buffer was removed by washing twice in PBSCM,and the cells were then lysed in ... Values are the mean of six replicates. The speciﬁc uptake was determined bysubtracting the uptake counts in the absence of inhibitorfrom the uptake counts in the presence of cocaine or S-cita-lopram, ... Todistinguish total binding from non-speciﬁc binding, cells wereincubated for 10 min in either PBSCM alone or PBSCMcontaining 200 lm cocaine for inhibition of DAT or 200 lmS-citalopram for inhibition...

Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

... N-terminal coiled-coil domain and a C-terminal ﬁbrinogen-like domain,both characteristic of angiopoietins. ANGPTLs do not bind to either the angiopoietin receptor Tie2 or the related protein Tie1, ... conditions leptin serves to counteract weightgain, in ammation induces a state of ‘leptin resistance’ in obese animals and humans resulting in development of hyperleptinemia. Similarly, hyperleptinemia ... endothelial cells maybe a mediator linking smoking to cardiovasculardisease in an autocrine or paracrine manner. BlockingANGPTL2 signaling may also be beneﬁcial also in preventing and treating...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... 2A). The zinc ions are requiredfor the structural integrity of the protein, as mutation of any of the cysteine residues involved in zinc coordina-tion results in protein unfolding [22]. The ... WDR5.Consistent with a central role for the MLL1 Win motif in the interaction by WDR5, substitution of arginine3765 with alanine in MLL1 abolishes the interaction between MLL1 and WDR5 [25,36]. Furthermore, ... element-binding (CREB) binding protein(CBP) [23], a peptide from the Win motif of MLL1bound to the WD40 repeat protein, WDR5 [24,25] and the C-terminal SET domain in the presence andabsence of histone...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... examined using the insulin precipitation assay. In the noncatalyzed assay, the disulﬁdes of insulin arereduced by dithiothreitol, causing the aggregation andprecipitation of the B-chain of insulin, ... B-chain signiﬁcantly,decreasing the lag-phase (Fig. 2A). When bacitracin wasincluded with PDI, the lag-phase of the insulin precipita-tion increased (Fig. 2A), with the effects increasing with increasing ... bacitracin is known to bind. These metal ions couldbe coordinated by the active site cysteines of the catalyticdomains of PDI, decreasing their activity. In addition,other thiols present in the...

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