... Watanabe,S.,Muramatsu,T.,Ao,H.,Hirayama,Y.,Takahashi,K., Tanokura, M. & Kuchino, Y. (1999) Molecular cloning of theLonproteasegenefromThermus thermophilus HB8 and char-acterization of its ... 811–819.28. Lanzetta, P .A. , Alvarez, L.J., Reinach, P.S. & Candia, O .A. (1979)An improved assay for nanomole amounts of inorganic phos-phate. Anal. Biochem. 100, 95–97.29. Farahbakhsh, Z.T., Huang, ... werepurchased from Sigma.DNA manipulation and sequence analysisPlasmid DNA preparation, purification of DNA fromagarose gel, and restriction enzyme analysis were performedby the standard methods...