... Cathepsins B, L and K, and hK1 bound C-BK peptide with a higher affinitythan the N-BK peptide (Table 1). The Michaelis constants for N-BK peptide were lower for cathepsins, and especially for ... lM)pluscathep-sins L (6 nM), K (4 nM)andB(3.7nM), and hK1 (4 nM). The Michaelis constants for hydrolysis of N-BK peptide byhK1 and cathepsin B were determined similarly, while the Km for ... correlatedwith BK metabolism and activity [23]. Taken together, thesedata point to the disruption of the cathepsin/cystatinbalance, during lung inflammation.In our efforts to characterize the proteolytic...