Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx

... slope of the curve found when the medium did not contain AdoMet, with an estimated half-life of 10.5 min. The role of the seven cysteine residues in L. donovani MAT was studied using the site-directed ... located in the central domain of eachsubunit, in the interface between the two dimers compri-sing the tetrameric structure [29]. This domain containsﬁve cysteine residues, two of them (Cys35 ... todetermine the functional role of the amino acid residues involved in each. The present report describes the cloningand subsequent functional refolding, using a bacterialexpression system, of the...

Báo cáo khoa học: Leishmania donovani bisubunit topoisomerase I gene fusion leads to an active enzyme with conserved type IB enzyme function doc

... lacks residues 1–174 of the N-terminaldomain and retains full enzyme activity in vitro. The enzyme contains a central DNA-binding core domainand a C-terminal catalytic domain harboring anSKINYL ... recon-stitution of the two recombinant proteins LdTOPILand LdTOPIS, corresponding to the large and smallsubunits, and localization of the active enzyme in both the nucleus and kinetoplast [7]. The enzyme ... subunit of the Leishmania enzyme, and the smaller catalytic subunit harbors the consensusSKXXY motif [8,23]. In the present study we alsoinvestigated the role of His453 of LdTOPIL in the transesteriﬁcation...

Báo cáo khoa học: Leishmania donovani phosphofructokinase Gene characterization, biochemical properties and structure-modelling studies doc

... positioning of model residues in unusual areas of the Ramachandranplot. The altered main chain conformation in the vicinitycauses the Gln287 side chain to intrude into the areacorresponding to the ... differences in the residues involved in nucleotide binding and three differences in the residues involved in the binding of fructose 6-phosphate. The sameFig. 3. Alignment of L. donovani and ... in both the active site and the region of the enzyme presumably involved in effector binding. Indeed, the differences in the effector-binding site tally with the apparently low level of activity...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... of the pro-tein in the N state and the evolution of the overallstructure of the protein in the MG state. This degree of resolution is unprecedented for the T domain of the diphtheria toxin. ... chaincorresponding to the catalytic domain (C domain);and the B chain corresponding to the translocationdomain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked ... hairpin TH8–TH9, which is responsiblefor the insertion of the T domain into the membrane. In the absence of the lipid bilayer, this hydrophobic part of the domain self-assembles, leadingto the...

Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

... regulates the flow of the Gibbs free energy inherent in C–C bonds in fatty acids into the production of either chemical energy or heat by directing the flow of iPLA2c into eith er the peroxisomal ... profiles and proteinchemical techniques including radiolabeling with [3H]BEL[7–12]. Study of human heart PLA2underscored the complexity of multiple distinct isoforms of iPLA2 in the cytosolic, ... recognized [15]. The localization of multiple iPLA2cisoforms to the mitochondrial compartment in myocar-dium in conjunction with prior work identifying the role of BEL-inhibitable iPLA2enzymes i...

Tài liệu Báo cáo khoa học: "Beefmoves: Dissemination, Diversity, and Dynamics of English Borrowings in a German" ppt

... Predicting the fate of anglicismsWe examine here factors hypothesized to play a role in the establishment (or decline) of anglicisms.Frequency in the English Covo corpus We ﬁrstexamine whether ... portion of the MZEE cor-pus, the training data consisted of the disjoint sub-sets of the English and German CELEX wordlists(Baayen et al., 1995), as well as the words used in Covo (to obtain coverage ... between stem frequency in Covoduring year t and frequency change in MZEE between tand year u = t + iInitial frequency and dissemination in MZEE In studying the fate of all words in two En-glish...

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... gene. The underlined amino acid residues are the signal sequences of the respective genes. The asterisk indicates the residues important for an interaction with the IL-10R/R1 receptor chain. The ... conserved cysteine residues in human IL-10 and IL-22 were found to be conserved in carpIL-10. The first cysteine forms a disulphide linkage with the third cysteine found on the D-helix. The second cysteine forms ... cytokines; interleukin; innate immunity; fish;expression analysis.Cytokines play a significant role in initiating and regulating the in ammatory process, which is an important defensesystem in innate...

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... the remaining b-strands, with the exception of strand 6, are arranged in two antiparallelbeta sheets within the a-crystallin domain. The inter-face between monomers of a dimer involves interactionbetween ... directly in the dimer–dimer interface. As a result of the spatial dis-position of monomers and their b-strand elements in the a-crystallin domain, the amino acid substitutionF112R introduces ... resem-blance, and the functional analysis mentioned previ-ously, indicate data obtained by examining bacteriallyproduced p26 are indicative of the protein synthesized in Artemia.Localization of amino acid...

Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc

... c10complex in the recombinant c ringpreparations suggests that the insertion of the last csubunit forms the limiting step in the assembly process of a functional oligomer.Upon closer inspection, the ... toclarify whether the stoichiometry of the c rings is in uenced by variation of the expression level of subunit c. In the present study we investigated whether the c subunits from the sodium F-ATP ... the size of the ring isnot changed by massive overexpression of subunit c in the E. coli host cells, indicating that intrinsic fea-tures of the monomeric unit determine the number of subunits...

Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf

... demonstrated that the serine protease inhibitor a-1 antitrypsin binds preprohepcidin within the cell during maturation. Furthermore, a-1 antitrypsin binds prohepcidinsigniﬁcantly in the plasma. This ... hepcidin iswith the iron exporter molecule ferroportin. We wereinterested in whether we could identify new protein–protein interactions of preprohepcidin, prohepcidinand hepcidin in vivo. For these ... of the proteins which contacted preprohepci-din interacted with the mature form of hepcidin in BacterioMatch screening. This further supports the possibility that these proteins may play a role...

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