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Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx

Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx

Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx

... The stop transfer sequence of the human UDP-glucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms Lydia ... fusion of UGT1A stop transfer sequence (TMD ⁄ CT)with the ectodomain of the plasma membrane CD4 reporter protein. Weshowed that the stop transfer sequence, when attached to C-terminus of the CD4 ... investigated the mechanism of ERresidency of UGT1A mediated by the stop transfer sequence by analysing the subcellular localization and sensitivity to endoglycosidases of chimericproteins formed by fusion...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... surface. They are found at the outermostends of the sugar chain of animal glycoconjugate. They actas an important component of the ligands recognized by the lectins. Recognition can be affected by ... until the A280 of the effluent was < 0.002. The column was further washed with LSB at 4 °C until the A280 of the effluent was < 0.002, and it was then transferredfrom 4 °Cto32°C and was washed ... 8.0).Preparation of BSM–agarose affinity gel The CNBr-activated Sepharose gel was prepared as instruc-ted by the manufacturer. The gel was then transferred to asolution of BSM (4 mgÆmL–1) and the suspension...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... 3¢end of the DBD) and SmRXR1 (Glu251 to Asn376)(containing 20 amino acids at 5¢ end of the DBD, the DBD and 40 amino acids at the 3¢ end of the DBD)were tested. Both SmNR1 (Ile247 to Ser372) and SmRXR1 ... regula-tion of a number of genes [7–10]. Study of SmRXRpartners will add to our knowledge of nuclear receptorgene regulation in schistosomes and to an understand-ing of the evolution of RXR’s ... mam-malian and snail hosts. Study of schistosome NRsenables us to understand how they regulate signalingpathways in the schistosome itself and to understand the molecular relationship between the...
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Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

... starvation-inducedspherulation of plasmodia and that then comprise 10% of the total mRNA [16]. Among them, spheru-Fig. 2. Structural alignment of amino acid sequence by motifs, of spherulin 3b with spherulin 3a and other ... ([6] and references therein), suggesting the presence of detect-able amounts of PMLA synthetase-specific mRNA. The absence of PMLA and polymalatase in other celltypes could be the result of cell ... spanned the nucleotides 51–310 of the gene including the origin of transcription, and was used as template forin vitro dsRNA synthesis as described by Donze and Picard[37]. In the case of NKA8, the...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx

Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx

... the conversion of long-chain fatty acyl-CoAs to acylcarni-tines in the presence of l-carnitine. This is the first stepin the transport of long-chain fatty acids from the cytoplasm to the mitochondrial ... or human CPT1B enzymes determines the overallmalonyl-CoA sensitivity of these enzymes. Thus, the N-terminal 50 amino acids of the human sequence increased the malonyl-CoA sensitivity of the ... inhibition To study the role of the N-terminal fragment of CPT1B enzymes, four human pig chimeras were con-structed by recombining pig and human CPT1Bsequences before (H50P and P50H) and after...
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Báo cáo khoa học: Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

Báo cáo khoa học: Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... Department of Microbiology and Immunology and the College of Medicine, by Public Health Service grant CA101931 (to D.J.M.) from the National Institutes of Health, and the AustralianResearch Council (to ... M. (1978)Oxygen and nitrate in utilization by Bacillus licheniformis of the arginase and arginine deiminase routes of arginine catabolism and other factors affecting their syntheses. J. Bacteriol. ... arginaseactivity (5200 U) (Fig. 2B). These results suggest thatarginase may lose the metal cofactor during preparation of the extracts, requiring reintroduction of the cobaltcofactor by heat activation.TransformedE....
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Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc

Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc

... cerevisiae;andSp,Schizosaccharomyces pombe.Only the t hree common sequence motifs are reported. The numbers in dicate the distances to the N term inus of each protein and the sizes of the gapsbetween ... regula-tive) of the mechanisms generating these species. Moreover,cN-II presents both phosphatase and phosphotransferaseactivities. Even though the physiological re levance of the phosphotransferase ... activated by ADP and BPG, and a synergistic stimulatory effect of these compounds was also observed. On the other hand, the slower migrating species (form A) (59 kDa), was activated to a greater...
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Báo cáo khoa học: A unique tetrameric structure of deer plasma haptoglobin – an evolutionary advantage in the Hp 2-2 phenotype with homogeneous structure pot

Báo cáo khoa học: A unique tetrameric structure of deer plasma haptoglobin – an evolutionary advantage in the Hp 2-2 phenotype with homogeneous structure pot

... carbohydratesassociated with the a-chain of either species (data notshown).Hypothetical model for the formation of the deerHp tetramer The ability of the deer Hp to refold and reassembleinto its tetrameric ... similar to that of human, with 142 (a2) and 245 (b) residues (Fig. 5A,B).A tandem repeat of the deer a-chain was observed(discussed below).Amino acid sequence alignment of deer and human Hp 2-2 The ... acid sequence analysis and divergence of mammal Hps. (A,B) Amino acid sequence alignment of the a- and b-chains of human and deer. Variable regions are shaded in black. The cDNA nucleotide sequence...
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Báo cáo khóa học: Structural and functional comparison of 15S- and 15R-specific cyclooxygenases from the coral Plexaura homomalla potx

Báo cáo khóa học: Structural and functional comparison of 15S- and 15R-specific cyclooxygenases from the coral Plexaura homomalla potx

... substrate. The Val349Ile mutant of the 15S-COXformed 65% PGF2a and 35% of the 15R-epimer of PGF2a.In the case of the 15R-COX, the Ile349Val mutation causeda more pronounced effect on the stereochemistry ... The correctorientation of t he cDNA insert in the v ector was confirmed by digestion with XbaI and the presence of mutations by digestion with HpaIorVspI.Protein expression and preparation of ... obtained by Swissprot on the basis of three-dimensional data of mammalian COX isozymes (datanot shown), reveals that the other 16 residues lie outside of the active site and mostly on the surface of...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... the Kdfor the initial bind-ing of minifiber. The fluorescence emission of the TMRwas assumed to be independent of the binding of the minifiber to the penton base, because it is attached at the ... (minifiber), and [Ligtot] is the total con-centration of the ligand. At any total concentration of lig- and, the anisotropy depends on the total concentration of C. Zubieta et al. Human adenovirus ... is the measured anisotropy value, AFis the aniso-tropy of the free minifiber, ABis the anisotropy of the mini-fiber bound to the penton base, [LigB] is the concentration of the bound ligand...
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