Báo cáo khoa học: Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis potx

... the target genes of micro-RNA124a (miR124a), and imaged the miR124a-mediated repression of chromosome 14 open reading frame24 (c1 4orf2 4, unknown function) during neurogenesis, using noninvasive ... miR 124 seed sequence of c1 4orf2 4 (Fig. 4C).To quantify the in vitro luciferase activity, represent-ing the miR124a-directed endogenous expression level of the c1 4orf2 4 gene during neurogenesis, ... AGCCMV/Gluc/c1 4orf2 4-3′ UTRCMV/Gluc/c1 4orf2 4-3′ UTRmtCMV/Gluc/C1 4orf2 4-3′ UTRCMV/Gluc/C1 4orf2 4-3′ UTRmt3′ UTR3′ UTRmtGlucCMVGlucCMVMutation of seed sequenceFig. 4. C1 4orf2 4 regulation by miR124a...

Báo cáo khoa học: SOX10, in combination with Sp1, regulates the endothelin receptor type B gene in human melanocyte lineage cells pptx

... presence of modifier genes for the EDNRB gene, and the expression of the modifier genes may beinfluenced by the environmental factors, thereby lead-ing to the phenotypic variability of WS4. One of suchmodifiers ... representation of the promoters of the EDNRB gene. The EDNRB gene is shown as a line and four arrows show the transcriptional initiation sites of each transcript. The openboxes of each transcript show the ... the fragment of the EDNRB gene (1 143 8 numbered in acces-sion number D13162), end-labeled with [c-32P] ATP atXcmI site, located at the position 143 8 in the antisensestrand of the EDNRB gene. ...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... [13,23,28,30–33]. The genetic evidencefor the role of AMPK in the hypothalamus is less clearbecause the loss of the AMPKa2 subunit in speciﬁchypothalamic cell types resulted in the opposite pheno-type ... entry of fatty acid into mitochon-dria. Thus, the steady-state level of malonyl-CoA inthese tissues is determined by the relative activities of ACC and malonyl-CoA decarboxylase.Role of the ... needs to beexplored further. Other areas of the brain have alsobeen shown to regulate feeding via AMPK [35,36]. Of interest is the extensive use of fructose as asweetener in the human diet [37]....

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... terminal in the sinus gland, as a result of late and progressiveisomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonaltract [39,41]. The immunohistochemical ... whereasseveral somas of the two types were dispersed in the X organ (Fig. 3A), most likely as a result of arte-factual displacement of the cell bodies during the prep-aration of the organ. In the sinus ... microscopystudy of the neuronal endings in the sinus gland todetermine the subcellular localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of enzyme(s)...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BHenzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range(pH ... andBHAb. The temperature dependence of the activities of the BHs was tested in the range 5–56 °C; the apparentoptimum temperatures of BHLmL2 were 25–30 °CTable 3. Masses and scores of tryptic ... cultures of L. macu-lans (twofold higher than that of camalexin). For thisreason, 3-phenylindole (0.2 mm) was used to induce the biosynthesis of BHs to facilitate their purification.Purification of...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... of the CA D. melanogastersequences and, on the other hand, the otherD. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano-gaster-3) or to the ... wasobserved in the cytoplasm of the cells. The stainingappears to be specific of the probe sequence because the staining is clear with the complementary sequenceto RpCAbr, but not with the sense ... amplifications of RpCAbr and RpCAtr with 18Samplification. The number of cycles on the y-axis is the differencebetween the number of cycles required to amplify each transcriptand the number of cycles...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... approximated by the sum of the probabilities of observing the set intermoleculardistances deﬁned in the 3D structure, relative to the likelihood of encountering such distance in the dataset of all protein–RNA ... onto the RNA bases nearest the center of the b-sheet structure of the RRM. We then scoredthese sequence-variant structures with the distance-dependent potential function.Figure 2 shows the ... case of the RNA muta-tions to the SRP complex, the mutated RNA residuesare located in a double-stranded region of RNA, and donot interact with the protein [54], yet the disruption of the helix...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... interruption of the geometry of the recognition helix for either Antp or the TTF-1HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion of helix III ... inertia.In the context of RSDM, the detailed analysis of the three spectral densities J(0), J(xN) and J(0.87xH)allowed us to obtain a rather complete description of the dynamics of the TTF-1 ... by RSDM calculation. The homogeneity of the values of the overall correlation time calculated from the individual (R2⁄ R1) ratiossuggested a good degree of isotropy of the global molecular...

Tài liệu Báo cáo khoa học: ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin docx

... autophosphoryla-tion of the Y166F mutant was similar to wild-type2-Cys Prx. Given the absence of another putative site,we approached the localization of the phosphoryl moi-ety by examining the mass spectra of ... 2-Cys PrxGiven the essential role of ATP in the peroxidaseactivity, we evaluated changes in the structure of 2-Cys Prx brought about by the concerted action of the nucleotide and the bivalent ... [23]. In the absence of perturbants, the predominant form of ACBDFig. 1. Effect of nucleotides ⁄ Me2+on the functions of 2-Cys Prx. (A) Concerted action of nucleotides ⁄ Mg2+on the peroxidase...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpiontoxins. Biochemistry 43, 1246 9– 1247 6.29 Yuan DD, Han YH, Wang CG & Chi CW (2007) From the identification of gene organization ... S5. The coelution of the synthetic conomarphinwith d-Trp14 (A) or d-Phe13 (B) with the naturalconomarphin.Fig. S6. Sequential daN(i,i +1)connectivities in the CaH-NH fingerprint region of the ... with other M-superfamily cono-toxins.Nevertheless, the cDNA structure of conomarphinwas similar to those of other conotoxin cDNAs. The cDNA-encoded precursor of conomarphin consisted of a conserved...

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