Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... level of Mcm expression in HeLa cells and cancer cells from human uterine cervix. Although it remains to be determined how enhanced expression of Mcm proteins affects DNA replication in cancer cells, ... in cancer cells derived from uterine cervix and in HeLa cells. The expression of Mcm genes in growth-arrested cells isinduced by addition of serum [43]. There are several E2Fbinding sites in ... signiﬁcance of Mcm proteins in the aberrant proliferation of cancer cells. In addition, Mcm3 and 4 proteins seem to be useful as amarker to discriminate cancer cells and dysplastic cells in theuterine...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... suggesting thatthe heterologous expression of genes encoding metallo- proteins can lead to incorrect metal ion incorporation.This is observed in rubredoxin and desulforedoxinswhere zinc, instead ... of protein uti-lizing the principle of protein-dye binding. Anal Bio-chem 72, 248–254.48 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature ... transformants.Protein techniquesProtein was determined by protein-dye binding with BSA asa standard [47]. Whole cell protein of C. ammoniagenes cells was isolated from 2 mL of induced culture....

Tài liệu Báo cáo khoa học: Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation pdf

... of the enzyme [7]. Having previously cloned themph gene from Ochrobactrum sp. M231 [7], we soughtto increase the thermostability of this MPH using pro-tein engineering.The two main protein-engineering ... Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of aglycine to proline mutationJian Tian, Ping Wang, Shan Gao, Xiaoyu Chu, Ningfeng ... September2010)doi:10.1111/j.1742-4658.2010.07895.xProtein thermostability can be increased by some glycine to proline muta-tions in a target protein. However, not all glycine to proline mutations canimprove protein thermostability,...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... Molecu-lar cloning and cold-inducible gene expression of ferritinH subunit isoforms in rainbow trout cells. J Biol Chem271, 26908–26913.13 Tang SJ, Sun KH, Sun GH, Lin G, Lin WW & ChuangMJ ... pattern of proportional pooling of subunitswith different kinetic and thermodynamic properties of the tetrameric enzymemay result in ﬁne-tuning of the properties of skeletal LDH-A, which is in linewith ... isoforms of acetylcholine esterase,choline esterase [10,11], ferritin H [12], ependymin [13],b-subunits of protein kinase CK2 [14,15], F0F1-ATPase[16], etc. Temperature-dependent gene expression...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... 161Determination of phosphatidylserine expression and cell deathPhosphatidylserine expression on the outer part of theplasma membrane was examined by Annexin V binding.Brieﬂy, cells were ... study showing the percentage of Perls’ positive HepG2 cells with time of culture in iron-rich media. A total of 200 cells were counted in each time point. (B) For ROS determination growing cells ... (thin lines) or with rab-bit Igs (thick lines) as ﬁrst step in permeabilized cells. (C) Histo-grams show ferritin expression in nonpermeabilized (thin lines,– saponin) and permeabilized cells...

Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... differentiationwas initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were ﬁxed on day 4 of differentiation and stained with hematoxylin and eosin. ... Wefurther investigated the kinetics of miR-27 expression during adipogenesis using quantitative real-time PCR.As shown in Fig. 1C,D, expression of both miR-27aand miR-27b decreased by ‡ 50% within ... notrepress the level of PPARc protein in committed prea-dipocytes under physiologically relevant conditions. Expression of miR-27 is elevated in obese mice In order to gain insights into the potential...

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... analysis of the puriﬁed proteins indicated thatthey contained < 5% contaminating proteins. The concen-trations of proteins used in these studies were determinedby spectrophotometric methods using ... overallactivity of chaperone proteins. Of the target proteins tested, Arg-HCl was found to speciﬁcally increase theactivity of aB-crystallin against DTT-induced precipi-tation of insulin at intermediate ... of insulin.ACBFig. 2. aB-crystallin protects against theDTT-induced aggregation of insulin, and thisactivity is enhanced by Arg-HCl. Insulin(, 0.25 mgÆmL)1) was incubated at 37 °Cin...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... proteins. B. cereus thus provides a convenient model organism forstudying proteins potentially associated with the pathogenicity of the highlyinfectious B. anthracis. The zinc-binding protein of ... determination of the BcZBPprotein at a resolution of 1.8 A˚, provides importantclues towards understanding the enzyme function,including the identiﬁcation of a zinc-binding site withina potential ... basecatalyst activating the zinc-bound water for nucleophi-lic attack. Interestingly, in the crystal structures of independently determined related proteins, the / ⁄ w-values of this Asp residue...

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... neutralizing action of the cytosolic RNaseinhibitor (cRI), a 50-kDa protein containing 16 leu-cine-rich repeat motifs [7,8]. In fact, onconase from Rana pipiens eggs [9], and seminal RNase from Bos ... of EIE were recorded at 30° inter-vals of r from 0 to 360°;(d) the inclination of the dimer major axis with respect tothe z axis, hence to the membrane plane, was changed (byinclination of ... cationic proteins, with highisoelectric point values. Thus we investigated the effectsTable 1. Natural and engineered RNases investigated in this study. IC50is the RNase concentration producing...

Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx

... levels within 24 h, indicating only transientdiminished expression of the PfG6PD-6PGL gene. PfG6PD-6PGL silencingcaused arrest of the trophozoite stage and enhanced gametocyte formation. In addition, ... to silencing the PfCLAG9 gene [23],inhibiting PfCLAG9 mRNA translation and diminish-ing cytoadherence of the protein to melanoma cells (afunction associated with this protein). Since our con-struct ... effectsdetected at 96 h in cultures transfected with vectorscontaining or lacking an insert indicate that after 48 h,other interacting factors could modify the molecularphenotypic effect of using this vector....

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