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the various parts of earth biosphere are connected and

Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot

Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot

Báo cáo khoa học

... shows that the members of this secondputative triad are in the correct relative orientation. Inour model, the Oc atom of Ser171, the amino group of Lys96 and the thiol group of Cys145 are sufficientlyclose ... bonds of differ-ent substrates [1]. These proteins all share the typicala ⁄ b hydrolase fold and can be grouped on the basis of their catalytic site and preferred substrate [2,3]. The enzymes of ... mutagenesis and their activity assayed with the colorimetric method of Berthelot, appropriately modified for the assay con-ditions of the archaeal enzyme. This assay is based on the measurement of the...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

Báo cáo khoa học

... calculated from the standard errorsassociated with the means of the measured values (i.e. bycalculating the square root of the sum of the associatedindividual errors squared). The maximum percentage ... aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde-pendent and different from that of the chaperone). The standard errors associated with this theoretical ... further enhanced by it (Fig. 1C). The sig-nificant decrease in the amount of precipitation in the presence of high concentrations of Arg-HClin the absence of the chaperone precluded analysis of the...
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The Health Benefits of Physical Activity for Girls and Women ppt

The Health Benefits of Physical Activity for Girls and Women ppt

Sức khỏe phụ nữ

... over the age of 65 are less active now than at the end of the eighties. Older women are the least active of all age and sex groups. In fact, middle-aged men and women, along with men over 65, are ... The exercise of daily life: Towards an understanding of the meaning and practice of physical activity in the daily lives of low-income single mothers. Masters Thesis. University of British Columbia: ... of the programs and policies related to physical activity and the health of girls and women are generally outside of the formal health care system. It is our intention to consider diversity, the...
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Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

Báo cáo khoa học

... ([6] and references therein), suggesting the presence of detect-able amounts of PMLA synthetase-specific mRNA. The absence of PMLA and polymalatase in other celltypes could be the result of cell ... substitutions, eight of them locatedin the first two b-strands of the N-terminal half of NKA48, upstream of the homodimer interface and accessible for interactions with other macromolecules.It ... affiliated withPMLA metabolism and ultimately to identify the syn-thetase gene, SSH was used with mRNA of the plas-modium as the tester and mRNA of amoebae as the driver. The amoebae strain chosen...
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Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

Báo cáo khoa học

... comparison of the effects of roughandsmoothLPSsofE. coli, both in the presence and absence of EDTA (Fig. 1). The rate of oxidation wasincreased in the presence of the LPSs of both the smooth and rough ... measure of the rate of oxidation of oxyHb.RESULTS The effect of pH on the auto-oxidation of cross-linked Hbwas studied. The rate of decrease of the concentration of oxyHb increased as the pH ... effective binding of Hb. The effects of the various LPSs on the oxidation rate of cross-linked Hb can becompared in Figs 1 and 2. The relative rates both in the presence and absence of EDTA are: smooth...
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A review on the cd content of rice daily cd intake and accumulation in the kidneys

A review on the cd content of rice daily cd intake and accumulation in the kidneys

Môi trường

... populations. Foods and beverages are sampled and bought in representativeretail shops in the area and analyzed for the element. The cadmium content of the same food item is averaged and multiplied ... content in soil and rice is low within the same area but high betweendifferent areas26).Daily cadmium intakeThere are four ways of estimating of daily cadmiumintake. The first is another meal ... zinc, and copper levels in the kidneys and liver of the inhabitants of north-eastern Poland. Pol J Occup MedEnviron Health 1993; 6: 133–141.41)/Orlowski C, Piotrowski JK, Kubów M. The levels of cadmium,...
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Báo cáo khoa học: The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXa reductase and human biliverdin-IXb reductase potx

Báo cáo khoa học: The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXa reductase and human biliverdin-IXb reductase potx

Báo cáo khoa học

... reduce the three other biliverdin isomers IXb,IXd and IXc. Bili-verdin-IXb reductase (BVR-B) catalyses the reduction of only the IXb,IXd and IXc isomers of biliverdin. Therefore, the activity of ... suggest that they may inter-act with the ‘roof’ and ‘floor’ of a hypothetical ‘verdinbinding site’, with the nicotinamide ring forming part of the floor. If the bound conformation is helical, therewould ... (B) The BVR-B reaction was initiated by the addition of hBVR-B (18.5 lg) and the spectra recorded over aperiod of 20 min.Fig. 2. The structures of the open chain and locked tetrapyrroles.The...
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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

Báo cáo khoa học

... study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also tostudy the effect of thermal unfolding in the presence and the absence of MgATP. ... esti-mation of the bandwidth and the intensity of the bandsmaking up the amide I or IÂ region. Thereafter, curve ttingwas performed and the heights, widths and positions of each band were optimized ... When the cat-f spectra obtained in the absence (Fig. 4A) and in the presence (Fig. 4B) of MgATP were compared the widening of the overallamide IÂ contour was less in the presence of MgATP and the...
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Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

Báo cáo khoa học

... amplitude and duration of signaling are controlled by the kinases or phosphatasesin the system, and whether they are controlled differ-entially by some or all of them. On the basis of the antagonistic ... how the second kinase in the cascade determines the time dependence of the activity of the third kinase, we varied the Vmax of the secondkinase reaction and recalculated the concentration of the ... such as the MAPK cascades, are often composed of kinase and phosphatase pairs.But how important are the kinases and phosphatasesrelative to each other? Are all combinations of the control of signal...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf

Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf

Báo cáo khoa học

... Ratiskol and C. Sinquin for the extraction and the preparation of the fucoidan fraction and for their experimental advices.We are grateful to Professor G. J. Arlaud for his generous gift of purified ... (CLR*).Incubation of T4 DNA with CLR* in the presence of fucoidan (CLR/fucoidan molar ratio of 1 : 20 and 1 : 40)leads to an inhibition of the binding of CLR* to DNA from the molar ratio 1 : 20. Altogether these ... and the CLR fragments of C1q werelabeled with fluorescent beads. The concentration of beadswas adjusted according to the type and the concentration of protein in order to maximize the rate of...
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