... shows that the members of this secondputative triad are in the correct relative orientation. Inour model, the Oc atom of Ser171, the amino group of Lys96 andthe thiol group of Cys145 are sufficientlyclose ... bonds of differ-ent substrates [1]. These proteins all share the typicala ⁄ b hydrolase fold and can be grouped on the basis of their catalytic site and preferred substrate [2,3]. The enzymes of ... mutagenesis and their activity assayed with the colorimetric method of Berthelot, appropriately modified for the assay con-ditions ofthe archaeal enzyme. This assay is based on the measurement of the...
... calculated from the standard errorsassociated with the means ofthe measured values (i.e. bycalculating the square root ofthe sum ofthe associatedindividual errors squared). The maximum percentage ... aB-crystallin (i.e. the effect of the additive on the aggregation ofthe target protein is inde-pendent and different from that ofthe chaperone). The standard errors associated with this theoretical ... further enhanced by it (Fig. 1C). The sig-nificant decrease in the amount of precipitation in the presence of high concentrations of Arg-HClin the absence ofthe chaperone precluded analysis of the...
... over the age of 65 are less active now than at the end of the eighties. Older women arethe least active of all age and sex groups. In fact, middle-aged men and women, along with men over 65, are ... The exercise of daily life: Towards an understanding ofthe meaning and practice of physical activity in the daily lives of low-income single mothers. Masters Thesis. University of British Columbia: ... of the programs and policies related to physical activity andthe health of girls and women are generally outside of the formal health care system. It is our intention to consider diversity, the...
... ([6] and references therein), suggesting the presence of detect-able amounts of PMLA synthetase-specific mRNA. The absence of PMLA and polymalatase in other celltypes could be the result of cell ... substitutions, eight of them locatedin the first two b-strands ofthe N-terminal half of NKA48, upstream ofthe homodimer interface and accessible for interactions with other macromolecules.It ... affiliated withPMLA metabolism and ultimately to identify the syn-thetase gene, SSH was used with mRNA ofthe plas-modium as the tester and mRNA of amoebae as the driver. The amoebae strain chosen...
... comparison ofthe effects of roughandsmoothLPSsofE. coli, both in the presence and absence of EDTA (Fig. 1). The rate of oxidation wasincreased in the presence ofthe LPSs of both the smooth and rough ... measure of the rate of oxidation of oxyHb.RESULTS The effect of pH on the auto-oxidation of cross-linked Hbwas studied. The rate of decrease ofthe concentration of oxyHb increased as the pH ... effective binding of Hb. The effects ofthe various LPSs on the oxidation rate of cross-linked Hb can becompared in Figs 1 and 2. The relative rates both in the presence and absence of EDTA are: smooth...
... populations. Foods and beverages are sampled and bought in representativeretail shops in the area and analyzed for the element. The cadmium content ofthe same food item is averaged and multiplied ... content in soil and rice is low within the same area but high betweendifferent areas26).Daily cadmium intakeThere are four ways of estimating of daily cadmiumintake. The first is another meal ... zinc, and copper levels in the kidneys and liver of the inhabitants of north-eastern Poland. Pol J Occup MedEnviron Health 1993; 6: 133–141.41)/Orlowski C, Piotrowski JK, Kubów M. The levels of cadmium,...
... reduce the three other biliverdin isomers IXb,IXd and IXc. Bili-verdin-IXb reductase (BVR-B) catalyses the reduction of only the IXb,IXd and IXc isomers of biliverdin. Therefore, the activity of ... suggest that they may inter-act with the ‘roof’ and ‘floor’ of a hypothetical ‘verdinbinding site’, with the nicotinamide ring forming part of the floor. If the bound conformation is helical, therewould ... (B) The BVR-B reaction was initiated by the addition of hBVR-B (18.5 lg) andthe spectra recorded over aperiod of 20 min.Fig. 2. The structures ofthe open chain and locked tetrapyrroles.The...
... study the secondary structure ofthe catalytic domain of PKCf and the effect of its substrate, MgATP, and also tostudy the effect of thermal unfolding in the presence and the absence of MgATP. ... esti-mation ofthe bandwidth andthe intensity ofthe bandsmaking up the amide I or IÂ region. Thereafter, curve ttingwas performed andthe heights, widths and positions of each band were optimized ... When the cat-f spectra obtained in the absence (Fig. 4A) and in the presence (Fig. 4B) of MgATP were compared the widening ofthe overallamide IÂ contour was less in the presence of MgATP and the...
... amplitude and duration of signaling are controlled by the kinases or phosphatasesin the system, and whether they are controlled differ-entially by some or all of them. On the basis of the antagonistic ... how the second kinase in the cascade determines the time dependence ofthe activity of the third kinase, we varied the Vmax of the secondkinase reaction and recalculated the concentration of the ... such as the MAPK cascades, are often composed of kinase and phosphatase pairs.But how important arethe kinases and phosphatasesrelative to each other? Are all combinations of the control of signal...
... Ratiskol and C. Sinquin for the extraction and the preparation ofthe fucoidan fraction and for their experimental advices.We are grateful to Professor G. J. Arlaud for his generous gift of purified ... (CLR*).Incubation of T4 DNA with CLR* in the presence of fucoidan (CLR/fucoidan molar ratio of 1 : 20 and 1 : 40)leads to an inhibition ofthe binding of CLR* to DNA from the molar ratio 1 : 20. Altogether these ... andthe CLR fragments of C1q werelabeled with fluorescent beads. The concentration of beadswas adjusted according to the type andthe concentration of protein in order to maximize the rate of...