the use of uv vis absorption spectroscopy for studies of natively disordered proteins
Báo cáo Y học: Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC Fluorescence resonance energy transfer and crystallographic study ppt
... the lack of a hydroxyl group of Ser146 diminishes the role of this residue in the stabilization of the substrate– ligand molecule, leading to the ÔconfusionÕ of the enzyme in the process of the ... The location of the remaining OA atoms is not very clear, due to the very weak electron density but the position of the first atom of the cis C9–C10 bond was used to model the remaining part of ... cis-PnA/NaTDC On the other hand, these shifts might result from the quenching of the tryptophan fluorescence in the presence of the mixed micelles, which simultaneously reveals the fluorescence of the tyrosine...
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Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction potx
... Table The probability of formation of various complexes with reference to the ratios between molecules A and B The Hardy–Weinberg law was used as the mathematical model for calculating the frequency ... resulting from the Ar -UV laser excitation) of the GFP and EYFP coexpressing cells The assay was also optimized for the measurement of FRET Excitation was carried out using an Ar -UV laser with the laser ... from the green and FRET channels were recorded at intervals of up to 20 after the addition of the GnRH agonist or antagonist to the final concentration of 100 nM [37] The average intensity of the...
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Báo cáo sinh học: "Combined optical trapping and single-molecule fluorescence" doc
... complementary bases, one on the 3 end of the shorter 15-mer and the other on the 5 end of the long strand; in these positions, the dye proximity causes fluorescence to be quenched A recent ... the bead from the center of the trap and thereby increasing the load until rupture (‘unzipping’) of the 15 base-pair duplex occurred Experiments were conducted in an assay medium consisting of ... count rates were further smoothed with a three-point boxcar filter Records of bead position were converted to force based on the measured stiffness of the trap The optical layout of our instrument...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... represent the active form responsible for recombination reactions [27] The presence of ATP was found to result in the formation of helical filaments on ssDNA, whereas in the absence of ATP there was ... together, these observations suggest that perhaps the ATP hydrolysis function of the protein may be related to some critical steps in the conversion of inactive forms of protein to that of active forms ... had either no nucleotide cofactor or had slowly hydrolysable analogues of ATP The rate enhancement by the presence of ATP was in the order of about 15-fold (Fig 6), strongly suggesting that the...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... receptors) These receptors are GPCRs that couple to G proteins of the Gi/o family There are two isoforms of the D2 receptor, D2Short (D2S) and D2Long (D2L), which derive from alternative splicing of the ... an oligomer There is also the question of the effects of G proteins on the oligomerization process In the insect cell system used in this report there is little interaction between the exogenously ... these authors, i.e the fusion of the receptor to a fluorescent protein, may have affected the conformation of these receptors Indeed agonist dose–response curves for the stimulation of [35S]GTPcS binding...
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Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy technique docx
... with the results from the X-ray crystallography of the VBC complex [26]; the distance between the C-terminal end of elongin B and the N-terminal end of elongin C used for the FRET pair of elongin ... although the effects caused by the binding of pVHL on the 3D structure of the elongin BC complex are not exactly known The present study has clarified that conformation of pVHL and ⁄ or elongin C in the ... elongin C Conformational changes of elongin C were caused by the binding of elongin B In the present study, we demonstrated that the fluorescence rise in the decay curves of acceptors can be used as...
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Báo cáo khoa học: Dimerization of mammalian adenylate cyclases Functional, biochemical and ¯uorescence resonance energy transfer (FRET) studies pot
... molecule, the 12 small black bars represent the 12 putative transmembrane segments of AC8 The names of the domains of AC8 are above the GFP/AC8 in the corresponding region Assays were performed ... Fig Tracking of the hydrophobic portion of AC8 (A) The left panel shows the structural diagram of 8Tm1/CFP/Tm2 The red and blue cylinders represent the transmembrane segments of the ®rst and ... with the formation of homomultimeric complexes of AC8 molecules We wondered whether a similar approach might reveal that heteromultimeric complexes could form between different isoforms of adenylate...
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SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES
... tether length plus the bead radius Bead fluctuation along the y-direction is used to measure the force The extension of the tether is determined from the center of the bead to the edge of the ... R is the radius of the arch and l is the length of the rod If we set E = k B T, then only the thermal energy induces the bend and with R = l we will get the length of the rod, along which thermally ... temperature, 𝑘𝐵 is the Boltzmann constant, is the end-to-end extension of the tether along the force direction, and the (𝛿𝑦)2 is the variance of the transverse (perpendicular to the force direction)...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... cytoplasmic area of the observed cells The decay curve of separately expressed cerulean–GAPDH and citrine–GAPDH (black) in the presence or absence of coexpressed PGK is also shown The shapes of the recorded ... are derived from the whole area (in the case of cell samples without coexpression of GAPDH) or from the cytoplasmic area (in the case of cell samples with coexpression of GAPDH) of cells, and are ... reduced by the coexpression of citrine–GAPDH, but coexpression of PGK did not affect the lifetimes Coexpression of citrine–GAPDH and PGK weakly decreased the lifetimes of cerulean–GAPDH in the cytoplasm,...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... rearrangement as a result of the activating twist in the ATDs of the CaR homodimer Further investigations into the signal transference from the ATDs to the G-protein coupling areas of the receptor homodimer ... Agonist-induced rearrangement of the 7TMs in the CaR homodimer? One of the goals of the present study was to investigate, whether the activating twist in the ATD dimer of the family C GPCR homodimer could ... (pBRET+) by the introduction of a Ser65 fi Thr mutation in the GFP2 part of the plasmid For the construction of the c-myc-CaR and HA-CaR constructs, a MluI site was introduced after the signal...
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Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
... activation and the reintroduction of calcium, as well as the effects of Tg on the components of these responses The peak amplitudes were calculated as the difference between signals before and after ... fusion proteins changes almost instantaneously following the disruption of PtdIns(4,5)P2 binding Therefore, the delay observed in our previous study indicates that the release of fusion proteins ... [20] To determine the physiological role of the calcium dependence of Ins(1,4,5)P3 metabolism, a more detailed analysis of the effects of calcium and the cross-talk between other signaling systems...
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FUNDAMENTALS of KINEMATICS and DYNAMICS
... and 7, the position of the bucket Thus, the final displacement of the bucket is controlled by two mechanisms: one is the mechanism for lifting the arm, and the other is for the rotation of the bucket ... the design process All of these will affect the size of the mechanism There are other considerations as well, such as, for example, what the allowable speed of approaching point B should be The ... determine the proper sizes of components, and thus to find the overall assembly dimension The spring force affects the reliability of the valve sealing If any of the requirements cannot be met with the...
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Atomistic modeling of energetics and dynamics of diffusive and frictional phenomena in c60 graphene based systems
... of the C60/graphene system for the Hex.-In Phase configuration (b) The corresponding contour plot of the PES The potential energy profile of the path in the [100] crystallographic direction of ... changing the temperature which alters the mechanism of exchanging the energy between the admolecule and the substrate We evaluate the effect of rotational degrees of freedom (DOFs) of the C60 ... coupling of adsorbate to surface 51B The substrate influences the adsorbate dynamics through the adiabatic PES of the system The difference between the value of the PES at the saddle point and at the...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays
... from 10 independent assays The average of the slope of six independent calibration curves was used as S for LOD and LOQ calculations The empirical method for estimation of the LOD and LOQ involved ... for days prior to the first blood sampling and for the duration of the study 2.5.2 Preparation of the Epimedium pubescens decoction The main study drug was a water decoction of Epimedium pubescens, ... liner The injection volume was 4.0 µL and the solvent delay was kept for The pulsed splitless mode was selected for sample injection with a pulse pressure of 60 psi and the purge time delay of The...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 2
... on the y-axis and estradiol concentrations on the x-axis, were then fitted with the regression method that visually 90 best fits the points within the range of the test samples that maximizes the ... hydroxycortisone in the presence of 50 pM of estradiol for a duration of days Changes of cell culture media were carried out twice on the second and fourth day of incubation Similar to what was observed for ... ER isoforms as they elicited the same maximum activation level of the luciferase reporter gene as estradiol Dose-response experiments with the standard estrogen, estradiol, indicated that the...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 4
... seen for ERβ, with the 189 exception of kaempferol, which suggests that the mechanisms of ERβ activation by kaempferol may differ from the rest of the phytoestrogens Positions 3’ of ring B and of ... underline the need for further evaluation of the relative significance of this estrogen to health in aging populations There was a consistent pattern of diurnal variation of estrogenicity in the subjects, ... 48 h The estrogenicity of these trace amounts of unconjugated icaritin was well below the detection limits of the ERα and ERβ, as well as, MCF-7 cell-based bioassays The low amounts of the compound...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 5
... respectively The increased 220 amount of prenylflavonoids in the latter extract was due to the increased solubility of the compounds in 70% ethanol that was used for extraction Table 23: Comparison of ... result of the use of different extraction methods, the profiles of prenylflavonoids ingested were markedly different, especially for icariin, which was nearly 80 times more abundant in the prenylflavonoid-enriched ... composition of constituents, the bioavailability of Epimedium compounds cannot be accurately ascertained from the results from both studies The duration of two studies and the number of metabolites that...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 6
... M., Morand, C., Mathevon, T., Besson, C., Manach, C., Scalbert, A and Remesy, C 2006 The bioavailability of polyphenols is highly governed by the capacity of the intestine and of the liver to secrete ... I.B., Mullen, W., Edwards, C.A and Crozier, A 2006 The relative contribution of the small and large intestine to the absorption and metabolism of rutin in man Free Rad Res 40(10) 1035–1046 Jefcoate, ... estrogen in the whole-body throughout mouse development Toxicol Sci 103(1):57-67 Moon, Y.J and Morris, M.E 2007 Pharmacokinetics and bioavailability of the bioflavonoid biochanin A: effects of quercetin...
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Application of formal and dynamic equivalence to the translation of bị and được into english
... information from the speaker to the hearer There might be a situation when the 21 speaker informs the hearer of the status of the road, warning the hearer not to use that road because it is being ... serve as real verbs when the subject is the agent of the verb In other words, the subject of the whole sentence and the subject in the predicate are uniform Consider the following examples: a ... to the standard of the system Then the speaker ironically expresses his/ her dissatisfaction about the road Another interpretation of the word bị may be attributed to merely a state of information...
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- bs 449 specification for the use of structural steel in building
- guidelines for the use of enteral and parenteral nutrition
- aspen guidelines for the use of parenteral and enteral nutrition 2009
- aspen board of directors guidelines for the use of parenteral and enteral nutrition
- clinical guidelines for the use of parenteral and enteral nutrition
- guidelines for the use of parenteral and enteral nutrition in pediatric patients
- guidelines for the use of parenteral and enteral nutrition in adults
- specification for the use of structural steel in building
- aspen guidelines for the use of parenteral and enteral nutrition 2002
- guidelines for the use of parenteral and enteral nutrition in adults and pediatric patients
- environmental impact of the use of fossil fuels for electricity generation
- the use of various types of nmr and ir spectroscopy for structural characterization of chitin and chitosan
- measurement and analysis of nmr residual dipolar couplings for the study of intrinsically disordered proteins
- circular dichroism techniques for the analysis of intrinsically disordered proteins and domains
- uv vis absorption spectroscopy and fourier transform infrared ftir spectroscopy
Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose