measurement and analysis of nmr residual dipolar couplings for the study of intrinsically disordered proteins

nmr methods for the investigation of structure and transport

Ngày tải lên : 29/05/2014, 18:32

... number of spins By linearity of the Fourier transform the direct relation between the peak integral and the number of spins of the corresponding chemical group holds for the spectrum of superposed ... derivation due to the time-dependence of the unit vectors Using the product rule for the derivation and the fact that the time derivative of the unit vector is the cross product of !rf with this ... expressions Qn and MCk the naturals n and k signify the index of the result and measured data vectors, respectively The latter results from the discretization of e.g kx in (2.38) For the sake of completeness,...

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Báo cáo y học: "Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pptx

Ngày tải lên : 11/08/2014, 05:21

... to the design of the study and planning of the work that led to the manuscript, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the ... letter to explain the background and aim of the study, and signed by the chair of the Dutch Society for Surgical Oncology The distribution of questionnaires, including the informative letters, ... facilitators to the implementation of the guideline for breast cancer surgery in day care The objective of our study was to investigate the complementary value of DCE for the evaluation of barriers and facilitators...

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báo cáo khoa học: " Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pot

Ngày tải lên : 11/08/2014, 16:21

... to the design of the study and planning of the work that led to the manuscript, the acquisition, analysis and interpretation of data, and has been involved in drafting and critically revising the ... letter to explain the background and aim of the study, and signed by the chair of the Dutch Society for Surgical Oncology The distribution of questionnaires, including the informative letters, ... facilitators to the implementation of the guideline for breast cancer surgery in day care The objective of our study was to investigate the complementary value of DCE for the evaluation of barriers and facilitators...

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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

Ngày tải lên : 18/02/2014, 06:20

... whereas only 40% of unordered conformations remained A NMR spectroscopy and resonance assignment The experimental conditions for the NMR study of VpUcyt in the presence of membrane-mimicking ... 39–81 of VpU The dotted lines mark the theoretical average values of the downﬁeld shift of 13Ca (top) and 13CO (bottom) as well as of the upﬁeld shift of 1Ha resonances (middle) characteristic of ... favoured and additionally allowed regions of the Ramachandran plot An additional 6% of residues were found in the generously allowed region None of the residues of helices and was found in the disallowed...

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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modiﬁcations and activity-based analyses doc

Ngày tải lên : 18/02/2014, 16:20

... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular application of ‘post-translational COFRADIC’, ... ‘light proteome’ and will only be present in the light 12C6-arginine form Almost identical numbers of pre-existing N-termini (i.e the N-termini of intact proteins) , on the other hand, should appear ... this study, 93 cleavage sites in 71 different proteins were characterized in a ‘proteomic background’ of more than 1800 proteins At the time of reporting these results, the overall majority of the...

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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx

Ngày tải lên : 22/02/2014, 08:20

... event of a bond has the weak, logarithmic dependence on number of serial bonds On the other hand, for the case (ii) where parallel bonds reside in the polymer chain, the force exerted for each ... which discards the thermal effect and the stretching rate effect, is sufficient to understand the role of folding topology in the mechanical behavior of protein materials as well as their mechanical ... representing the degree of folding topology of proteins For a protein with N residues, the degree -of- fold, Q, is defined as Q = Nc/(N(N – 1)/2), where Nc is the number of native contacts and N(N –...

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Báo cáo khoa học: Afﬁnity puriﬁcation-mass spectrometry Powerful tools for the characterization of protein complexes ppt

Ngày tải lên : 31/03/2014, 07:20

... 571 Analysis of protein complexes Within the scope of this review, we will focus on summarizing the current technical state of the art for the biochemical retrieval of associated proteins and ... activity of the spliceosome is the recognition of splice sites in nuclear pre-mRNA and to catalyze the accurate removal of introns and ligation of exons to yield a mature mRNA The affinity of the splicing ... streptavidin-matrix The protein content of the purified complex was then separated on a 2D gel and analyzed by mass spectrometry An example for the use of peptides as affinity ligands is the study of Husi...

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Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx

Ngày tải lên : 18/06/2014, 19:20

... drafted the manuscript AF conceived the study, designed and conducted the study and drafted the manuscript AS collected the samples and reviewed the manuscript IT participated in design of the study ... Department of Radiotherapy, Charité, Berlin, Germany 12 Authors’ contributions ZL conceived the study, collected the samples, carried out assays and measurements, performed the statistical analysis and ... complexes The complexes recognize both dextran and the Fitc-molecule of the EpCAM antibody The cell suspension was brought to a total volume of 2.5 mL, and the tube was placed into the previously...

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báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx

Ngày tải lên : 20/06/2014, 03:20

... drafted the manuscript AF conceived the study, designed and conducted the study and drafted the manuscript AS collected the samples and reviewed the manuscript IT participated in design of the study ... Department of Radiotherapy, Charité, Berlin, Germany 12 Authors’ contributions ZL conceived the study, collected the samples, carried out assays and measurements, performed the statistical analysis and ... complexes The complexes recognize both dextran and the Fitc-molecule of the EpCAM antibody The cell suspension was brought to a total volume of 2.5 mL, and the tube was placed into the previously...

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báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx

Ngày tải lên : 11/08/2014, 00:22

... and N-terminal α-amine) are present on many proteins This ultimately Another drawback for the use of EDCHCl/sulfo-NHS for the formation of stable bioconjugates is the lack of specificity on the ... time and temperature are dramatically different (90 at 37°C for cysteamineHCl and 30 at room temperature for DTT) Furthermore, the use of 50 kDa MWCO centrifuge filters allowed for retention of the ... of the conjugate The increase in peak width of the QD-BSA can be attributed to a number of factors, including the polydispersity Page of 15 (page number not for citation purposes) Journal of...

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báo cáo khoa học: " A new model for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation" pptx

Ngày tải lên : 11/08/2014, 20:21

... 5.0 g of barium titanate were then distributed in the dust chamber prior to each shot, and a filter paper was inserted to block the dust chamber from the barrel of the rifle The other end of the ... differences in the diameter of the cavity between the two projectiles were found 6.0-10.0 cm behind the point Page of of impact of the projectile on the gelatin block In this area, the gelatin blocks ... within the ruptures of a temporary cavity in the gelatin block revealed a deposition of particles along the entire bullet path for both types of projectiles examined In the case of the soft point...

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Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis" pdf

Ngày tải lên : 12/08/2014, 17:22

... included in the assays using the CID 1, CID 3, CID 4, and bone chips, and levels of each analyte were calculated on the basis of the standard curves generated Results for the synovial antigen and chips ... buffers, we diluted the serum samples 1:10 for use in the synovial antigen and 2, CID 3, and CID chips, and 1:100 for use in the CID chips In the assays using the synovial antigen and 2, CID 1, CID ... determinations, including standards and controls Page of 13 The chips and markers used in the present study are listed in Table 1; the sequences of the peptides spotted onto the chips are listed in...

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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

Ngày tải lên : 24/08/2014, 09:51

... (Cys243) of ALDH3A1 and inhibits it activity (Khanna et al., 2011) The assay was carried out for 15 minutes and then the rates of the control and inhibition reactions (384 points for each set of reactions) ... calculate the Z’ factor using the following formula: Z’ factor Where δp= standard deviation of the control reaction, δn= standard deviation of the inhibition reaction, µp= average of the points in the ... transfer The activated water performs a nucleophilic attack on the carbonyl carbon displacing the carbon–sulfur bond and releasing the reduced cofactor NAD(P)H and product carboxylic acid The order of...

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guide for structural maintenance of parking structures

Ngày tải lên : 24/10/2014, 21:59

... taken and a variety of tests performed The most important tests are those that determine the extent of corrosion and bond loss of the reinforcement and those that quantify the amount and extent of ... mechanism of joint sealants is deterioration of the surfaces to which the sealant is bonded, allowing the intrusion of water and subsequent progressive failure of the sealant along the length of the ... the length of the joint is preferable This will reduce the probability of catching the plow on the edge of the joint Snowplow damage normally is not covered by the seal system warranty, and the...

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the identification and characterization of proteins required for endocytosis in the budding yeast saccharomyces cerevisiae

Ngày tải lên : 14/11/2014, 06:20

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Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films

Ngày tải lên : 10/09/2015, 08:41

... where dc and Lc are the width and the length of the cantilever, hc and α are the gap and the angle between the cantilever and the sample surface, respectively Equation (2.2) describes the non-local ... industrial corporations, and make the PFM an important tool to study these properties in depth 2.3.1 Thermodynamics of Domain Switching The study of the thermodynamics and kinetics of the switching process ... important for the applications of PFM for high-density data storage and ferroelectric 28 Chapter lithography Abplanalp [7] gave the first analysis for the thermodynamics of domain switching and the...

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Structural characterization of nogo proteins implications for biological functions and moleculedesign of therapeutic applications

Ngày tải lên : 14/09/2015, 12:53

... 3.3.4 CD and NMR characterization of the Nogo-B N-terminus 99 Figure 3.3.5 CD and NMR characterization of the Nogo-33 101 Figure 3.3.6 CD and NMR characterization of the first group of the dissected ... block the interaction between the ligand and the receptor The peptide NEP1-40, consisting of the first 40 residues of the Nogo-66 loop of Nogo-A , behaves as an antagonist of NogoA to attenuate the ... Bioinformatics analysis 93 3.3.2 CD and NMR characterization of N- and C-termini of Nogo-B 97 3.3.3 CD and NMR characterization of the dissected Nogo-A fragments 3.3.4 Discussion 107 Identification of the...

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Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx

Ngày tải lên : 10/12/2013, 18:15

... contaminants and aging Also meets standards for environmental cycling for impulse and AC surges • Meets GR-63 standards for transport and storage • NEBS Level compliant • Insertion loss of less than ... be isolated and easily replaced • Meets all standards for controlled environments including temperature, humidity, office vibration and airborne contaminants • Meets all standards for uncontrolled ... replacement for protecting inputs and outputs of DSLAM equipment 7/07 • 1 A E Solid-State ComProtect® • Both 24- and 26-gauge wire can be used to create a fuse link from the protection field to the...

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Fundamentals of quantum mechanics for solid state electronics, optics c tang

Ngày tải lên : 17/03/2014, 14:41

... that there must be an ‘‘equation of motion’’ that governs the time-dependence of the state of the system The mathematical solution of the equation of motion for the dynamic variables of the system ... of the two properties without influencing the outcome of the measurement 14 Basic postulates and mathematical tools of the other property, and vice versa In short, the order of measurements of ... the action is specified in terms of the force acting on the system Since the force is equal to the rate of decrease of ~ the potential energy with the position of the system, or F ¼ ÀrVð~Þ, the...

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nanomaterials for solid state hydrogen storage, 2009, p.346

Ngày tải lên : 04/06/2014, 14:37

... of Development of Hydrides and Nanomaterials 19 synthesis of the elements and the first determination of values for the decomposition pressure on temperature, the enthalpy of formation, and the ... bottom of the cylinder, to the o’clock position, and then falls to the bottom of the reactor The point where each ball is detached from the wall is well determined by the position of the magnet; ... motion of the balls inside the shell is well controlled by the external magnets to the point that the fraction of the impact and the shear modes of mechanical milling can be controlled The highenergy...

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