... during organ transplantation by elevating the concentration of cytosolic biliverdin-IXa. The pau-city of information on the biliverdin binding site hashampered the development of inhibitors of ... this in mind, and inthe absence of a crystalstructure ofthe ternary complex of BVR-A with pyri-dine nucleotide and biliverdin, we set out to furtherprobe the nature ofthe biliverdin binding ... Because ofthe absence of acrystal structure with biliverdin bound to BVR-A, we have investigatedindirect ways of examining tetrapyrrole binding. Inthe present study, wereport that a number of...
... spinal laminar lineFigure 1 The sagittal diameter ofthe spinal canal (a) is meas-ured from the posterior point ofthe corresponding spinal laminar line. The sagittal diameter ofthe vertebral ... the C3/4 level is stronglyassociated with the development of palsy. Since anatomi- The sagittal diameter ofthe spinal canal (a) is measured from the posterior point ofthe corresponding spinal ... instead of normal,which justified the transformation ofthe Average Pavlovratio into a categorical variable. We used the 1st quartile as the cut off for defining extremely narrow spinal canal,...
... should prove useful instudyof the response to infectious agents and vaccines [8]. ACT1 isexpressed within 16 to 24 hrs of stimulation with con Asimilar tothe kinetics of appearance of CD25. ... Cells were thendistributed in 50 àl aliquots to wells of 96-well V-bottommicrotiter plates containing either 50 µl of PBS-HS(negative control) or 1 µg of mAb in 50 àl of PBS-HS andincubated ... herd maintained by Dr. H.L. Popenoe at the University of Florida (Gainesville, FL) served as a source of peripheralblood used in this study. Monoclonal antibodies: The mAbs used in this study (Table...
... Espat*AbstractBackground: Hyperthermic chemotherapy applies thermal energy to both abdominal wall as well as the intra-abdominal viscera. The combination ofthe hyperthemia, chemotherapy and cytoreductive surgery ... regardless ofthe origin of the primarymalignancyandthebodysurfaceareaofthepatient [23]. The present protocol does not include ameasurement of serum Mitomycin C levels thus we areunable to discuss ... reinforcement tothe abdominal wall with anabsorbable material resistant to infection and withpotential remodeling to host own tissue. Therefore, weused a biomaterial mesh to reinforce the...
... purposes)IL-6 cytokines, the important mediators of fatal septicshock.Thus, the development of muti-epitopic E. coli and S.aureus vaccines using these adjuvants appears promising. The other area of interest ... 10-day intervals. The efficacy ofthe phytol and PHIS-01, as compared to IFA,was assessed by ELISA in terms of anti-bacterial antibody and inflammatory cytokines. We alsoexamined the ability ofthe ... induction of inflammatory cytokines, such as IL-6 and/or TNF-α, the levels of these cytokines were measured in peritoneal lavages. The results obtained from mice chal-lenged with 106 CFU of E....
... significantly lower inthe P. tinctorius COI 024treatment than inthe control and G. aggregatum IR 27treatments (table III). The type of fungal symbiosis influ-enced the mineral contents ofthe leaves ... comparedwith single inoculation. However wecannotattribute thisstimulation only tothe mycorrhizal symbiosis because of the presence of nodules on the ecto/endomycorrhizalseedlings. The ability of A. ... biomass). The response tothe dual inoculation was greater than the response to either inoculant one. It may be due tothe fact that the co-inoculated plants formed nodules through contaminations....
... 6:54http://www.nanoscalereslett.com/content/6/1/54Page 10 of 13 NANO EXPRESS Open Access Atomic ForceMicroscopyStudyof Protein–Protein Interactions inthe Cytochrome CYP11A1(P450scc)-Containing Steroid Hydroxylase SystemYD ... gain a betterinsight into the intrinsi c mechanism of electron transfer in this system, it is necessary to have information on the stru cture and properties of individual proteins and theircomplexes. ... this study, the AFM detector was used to control the monomeriza-tion procedure of CYP11A1 as well as to visualize andmeasure the sizes of single protein molecules and theircomplexes within CYP11A1...
... volume ofthe Au nanometricgrains forming the film can be obtained. The XEI softwarefor the analyses ofthe AFM images allow to obtain the distribution ofthe grains radii, R, and ofthe grains ... room-temperature growth of sputtered Au filmson SiO2using theatomicforcemicroscopy technique. By the analyses ofthe dependence ofthe roughness, r, of the surface roughness power, P(f), and ofthe correlationlength, ... procedure consisting inthe definition of each grain area by the surface image sectioning of a plane that was positioned at the half grain height. As a consequence, the distribution of the grains radii...
... that the hinge region of IgG links the two Fab arms tothe Fc portion, provid-ing global flexibility tothe IgG. The flexibility of the IgG molecule results in Fab ‘elbow bending’, Fab ‘armwaving’ ... lumi-nal side ofthe membrane. (C) Histogram of the protrusion heights determined using the AFM image in (A). (D) Histogram ofthe pro-trusion widths determined using the AFMimage in (A). (E) ... ratio ofthe total amount of amino acids on the cytosolic and luminal sides was calculated as 2.31 : 1(data not shown), verifying the efficacy of this tech-nique. The high protein density in 2D...
... assay for molt inhibition [4]. Except for the role of CHH inthe increase of glycemia, theuse of recombinant protein tostudy molt-inhibiting functionand gonad maturation remains a challenge ... amount of dsRNA injected inthe animal mayvary, depending on the expression level ofthe gene; amuch higher dose of dsRNA was injected into the shrimp L. schmitti to silence the CHH gene [26]. In our ... sourcefor the final synthesis of vitellin. The newly made vitel-logenin is expected to be secreted rapidly into the hemolymph and transported tothe ovary for oocyteuptake. To demonstrate that the increase...
... to investigate the electrical transport properties ofthe MWCNTs. The dispersion solution containing CNTs was then depositedonto these substrates. Atomic force microscopeFor all the experiments ... Rtip-substrateby using the Sharvin resistance model. Our final objective is thus to deduce the CNT intrinsic radial resistance taking intoaccount the calculated electrical resistance values with the global ... all the AFM measurements as well as all the theoreticalcalculations, participated inthe design ofthe study, wrote the manuscript,and coordinator between all the participants. LO made all the...
... crystalline Au deposition because the small mis-match ofthe crystal lattice allows the Au to grow in largeatomically flat areas. The understanding ofthe kineticgrowth mechanisms of Au on ... by12112NyyiiN()/where N is the number of data points ofthe profile, yiare the data points thatdescribe the relative vertical height ofthe surface, andyis the mean height ofthe surface. Furthermore, the roughness ... coordination;performed the gold sputter deposition, the annealing processes and the atomicforcemicroscopy analyses; developed the theoretical framework for the analyses ofthe experimental data; analyzed the...
... showed promising benefits in a num-ber of areas, including intheuseof lay health workerprogrammes to increase breastfeeding and childhoodimmunization. However, the results within these sub-groups ... directly influence the success and sustainability of this programme. These issues included the lay health workers’ perceptions ofthe teaching methods; their perceptions ofthe incentives given; their ... factors or interve ntion characteristicsthat may have influenced the trial results. In principle,qualitativ e research can also be used to shed light on the findings of systematic reviews of the...
... explored in the EXACTLE study and the findings are reported here. Theseincluded the identification ofthe most discriminatingdensitometric index for use as an outcome measure. Fur-thermore, the ... study) , in the writing ofthe manuscript, inthe decision to submit the manuscript for publication. RS was responsible for the UK arm of EXACTLE and reviewing/contributing to writ-ing the manuscript. ... was used for the voxel index as for PD15 in this study. Sensitivity ratios were determined for each ofthe densito-metric indices by dividing the value for the mean changefrom baseline in lung...