... language of instruction in HongKongHong Kong: The Cosmos Cheung, HN 1972 A Grammar of Cantonese Spoken in HongKongHong Kong: The Chinese University Press Curriculum Development Council ofHongKong ... general use of government funding on the promotion ofthe “biliterate and trilingual” policy sufficiently reflects the mainstream view on the teaching language oftheHongKong society Besides the regular ... stage of education The Language Fund of SCOLAR was established to support proficiency in the use ofthe Chinese (including spoken Putonghua) and English languages by the people ofHong Kong, ...
... language of instruction in HongKongHong Kong: The Cosmos Cheung, HN 1972 A Grammar of Cantonese Spoken in HongKongHong Kong: The Chinese University Press Curriculum Development Council ofHongKong ... general use of government funding on the promotion ofthe “biliterate and trilingual” policy sufficiently reflects the mainstream view on the teaching language oftheHongKong society Besides the regular ... stage of education The Language Fund of SCOLAR was established to support proficiency in the use ofthe Chinese (including spoken Putonghua) and English languages by the people ofHong Kong, ...
... removal ofthe second half ofthe PolC N-terminus would help to improve the results further Therefore, we took only the fragment ofthe multiple sequence alignment covering the first half ofthe PolC ... explore the structure ofthe PolC N-terminal region We found that this region includes two consecutive structural domains Both domains are distantly related to the structure of domain V ofthe clamp ... Interestingly, the first half ofthe PolC N-terminal region ( 1–100) also detected the PolIIIs-V domain, albeit weakly (hhsearch probability of 16%) The structures of PolIIIs-V and DnaA-I adopt a variant of...
... GM2 and the interaction ofthe GM2AP ⁄ GM2 complex with the enzyme To delineate in detail the separate effect ofthe introduced mutations on the lipid- and the enzyme-binding of this cofactor, ... that ofthe WT protein Only these variants of GM2AP were examined in all further studies To assess the potential ofthe variant GM2AP forms for the stimulation of HexA catalysed hydrolysis of GM2, ... nomenclature; they assign the number to S32, which represents the N-terminus of mature GM2AP.) This interaction might be crucial for stabilization ofthe position ofthe glycolipid within the spacious...
... region Effect of mutations in the C-terminal regionofthe RmInt1 IEP on RNA splicing in vivo We constructed a series of mutants to identify the functional features ofthe C-terminal regionof ... with the high-affinity binding site in subdomain DIVa ofthe intron, whereas other regions ofthe RT and domain X interact with conserved catalytic core regions [12] Domain X is located in the ... DNA-binding region, an extension of domain X, or simply a nonfunctional extension In the present study, we investigated the C-terminal regionofthe RmInt1 IEP up to the maturase domain and the C-tail...
... selfimage ofthe scientific world as ofthe perception of society at large that visible effects of research in technological and finally economic terms are part ofthe matter Any concept ofthe law of ... Representatives ofthe Contracting Parties named in the preamble to the present treaty shall meet at the City of Canberra within two months after the date of entry into force ofthe treaty, and thereafter ... Consequently, the task of defining the term is part ofthe effort to design the subject matter itself There is no positivist approach or else given idea to the concept of international law of science...
... part ofthe coenzyme B-binding site Its unmethylated nitrogen atom Ne2 donates the shortest ofthe three hydrogen bonds ofthe protein to the phosphate group of coenzyme B The distance of ˚ 2.65 ... digestion ofthe a-subunit with trypsin, the generated peptides were analyzed by MALDI-TOF MS The peptide with a mass of 2446.36 Da (predicted to contain the thioglycine) was then analyzed by MALDI-TOF ... because the genes required for the biosynthesis ofthe nickel cofactor F430 [16] and for the post-translational modifications [9] are not yet known [17,18] Also, the in vitro reconstitution of active...
... structural changes in the myosin head The main goal of these studies was to understand the mechanism of these changes, i.e the mechanism of transmission of structural changes from the nucleotide- and ... accelerate the inactivation ofthe S1 ATPase upon mild heat treatment with the loss ofthe ability of nucleotides to protect the S1 against thermal denaturation [23] There is some discrepancy in the ... obtained by addition of 0.5 mM BeCl2 in the presence of mM NaF The formation ofthe complexes was controlled by measuring the K+-EDTA ATPase activity ofthe protein The ATPase activity of S1, t-S1, or...
... to +1157 relative to the AUG ofthe psbA mRNA (corresponding to the coding regionofthe C-terminal amino acids of D1 and the 3¢ UTR ofthe psbA mRNA) was amplified with the oligonucleotides psbA3/1 ... fractions of 500 lL; 10 lL of these fractions were used for UV cross-linking experiments In vitro synthesis of RNA and UV cross-linking of RNA with proteins Templates for the in vitro synthesis of psbD ... preferentially binds to the 5¢ UTR ofthe psbA message determined by an A-rich region eight nucleotides upstream ofthe AUG start codon To the best of our knowledge, this is the first RNA binding...
... the wild-type strain With L1, L4, LH2, HM or LH5, 50% to 80% ofthe cells had foci With LH3, 40% ofthe cells had foci LH1 and LH4 greatly hampered the formation ofthe foci and most ofthe ... Mutations on the flexible linker regionof MukF affect the detachment of MukF C-WHD from engaged MukB heads For a biochemical assay ofthe detachment of MukF C-WHD from the MukB head, we employed the MukE–MukF(M+C)–MukBhd ... ofthe symmetric dimer was observed (Fig 3A; the band labeled as ‘sym’), apparently showing that they affect the detachment ofthe MukF C-WHD from engaged MukB heads The band intensities of the...
... testing the hypothesis that the N-terminal regions of TTR affect the affinities of TTR for thyroid hormones A previous study revealed that altering the structure ofthe N-terminal region influenced the ... primary structure ofthe N-terminal regions of TTR and the biological function of TTR 4014 Results Synthesis of recombinant human TTR In the crocodile, TTR is only synthesized in the liver during ... Examination ofthe alignment of TTR amino acid sequences from 20 vertebrate species revealed that theregionofthe subunit which changed in a distinct and directed manner was the N-terminal region The...
... ofthe TjZNT2 N-terminus maintained its ability to complement the mutant Exchange ofthe TM III–IV loop (L region) , one ofthe least similar regions between the two proteins, did not affect the ... with the exception ofthe N-terminus and the TM III–IV loop To examine the functions of TjZNT1 and TjZNT2, these transporters were tagged with hemagglutinin (HA) at their N-termini to verify their ... that is not present in the sequence of TjZNT1 The present work provides several pieces of evidence for the involvement of this N-terminal region in the ion selectivity of TjZNT2 and related proteins...
... expressing the recombinant mPC1 ⁄ 3, the presence ofthe 87 kDa form in excess ofthe 66 ⁄ 71 kDa facilitates isolation ofthe enzyme and helps in maintaining the enzymatic activity at a proper level The ... However, the proregion is a potent inhibitor of PC1 ⁄ and binds the active site with nm affinity in vitro resulting in the formation of a stable proregion–enzyme complex [5] Additional cleavage ofthe ... liter of bacterial culture Effect ofthe CT-peptide on mPC1 ⁄ enzymatic activity The effect of various concentrations ofthe purified CT-peptide on the cleavage ofthe fluorogenic substrate pERTKR–MCA...
... directly the role of CHD3 in the regulation of PS1 gene in vivo We first 1440 Fig Effect of N-terminal deletions of CHD3 on the inhibition of transcription by CHD3 and on the binding of CHD3 with ... several of these corepressors also interact with the C-terminal regions of CHD3 downstream of residue 1676 [46] or the conserved regions of CHD4 and dMi2 [50] Models proposed to explain the repression ... sequence with the central consensus 5¢-GGAA ⁄ T-3 (Fig 1A) [18,20] Based on the sequence homology ofthe ETS domain, and the conservation of other functional domains, a phylogenic tree ofthe ETS gene...
... excess of a nonspecific RNA, the c-myc coding region, had no effect on binding (results not shown) These data indicate that the 36 kDa protein bound to the 194–280 regionofthe 3¢UTR These observations ... localization of mRNAs such as b-actin and MBP Here we describe the specific binding of a trans-acting factor to theregionofthe 3¢UTR of c-myc mRNA previously shown to contain the localization ... proteins and one other minor component Comparison ofthe mobility of these proteins in SDS ⁄ PAGE with that of molecular mass standards indicated that the major proteins were of approximate molecular...
... at early NGG codons is the reason for the observed low gene expression The length ofthe peptidyl moiety ofthe peptidyltRNA influences the drop-off process in the case of very small mini-genes ... inhibit growth ofthe strain even in the presence of IPTG induction All of these results suggest that the low gene expression associated with NGG codons in the downstream region following the initiation ... different from the related codon AGG The other NGG codons CGG and UGG were not analysed for the influence of increased concentration of cognate tRNAs The question remains whether the plasmid associated...
... perfectly match their theoretical values, assuming formation of all seven disulfide bonds in the sPLA2 molecule Influence ofthe mutations on the secondary structure and overall conformation ofthe AtnI2 ... demonstrated the importance for neurotoxicity ofthe C-terminal region stretching over the top ofthe Atx molecule (Fig 2), particularly certain hydrophobic/aromatic and basic residues [21–23] The same region ... in the Cterminal, AtxA-like region As shown by the studies ofthe AtxA double mutant (K108N/K111N) [21,22], the influence of K108N substitution on neurotoxicity and protein-binding properties of...
... number ofthe diverse superfamily of GPCRs, not least among the prostanoid receptor subfamily of GPCRs [53] The EP3 subtype ofthe PGE2 receptors is one ofthe most heavily spliced among all ofthe ... by either the presence of a TI site for the TPb mRNA transcript at )12 or due splicing of nucleotides elsewhere in the 5¢ flanking regionofthe TP gene to nucleotides located within the )12 region ... the current study, we sought to investigate the molecular basis ofthe differential expression of TPa and TPb isoforms Our initial aim was to map the patterns of exon usage in the 5¢ UTR of the...
... deletions investigated, with the exception of deletion R30hE5, increased the number of colonies and the colony size of keratinocytes growing in soft agar The effect ofthe empty vector on colony ... The results shown in this communication demonstrate that the expression of theHPV16 E5 protein increases the number and the size of HaCaT cell colonies growing in soft-agar The results were the ... but determines the number of cells and therefore the colony size (see Fig 6) The data here detailed represent the first quantitative description ofthe ability of HPV16 E5 to Page of 10 (page number...
... to the reliability ofthe House Finance Office’s Statement of Accountability and the effectiveness of internal controls The computer system serving the Office of Finance is maintained by the ... disbursements ofthe Director, House Office of Non-Legislative and Financial Services, for appropriated and other funds arising from cash transactions ofthe House Office of Finance for the fiscal ... Other Funds arising from cash transactions ofthe Office of Finance, House of Representatives, for the fiscal year ended September 30, 1993 The House Office of Finance performed receipt and disbursement...