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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... [29].125I-Labeling of LDL was per-formed, prior to other modification, using iodine mono-chloride [36,60]. Specic activity (typically 50100c.p.m.ặng)1apoB protein) was determined by c-counting(Cobra ... times. For turnover studies, the [125I]LDL-containing medium was removed after the accumulation phase. The cells were then washed withwarm NaCl Pi, and medium containing 1 mgặmL)1BSA was ... peptides) in the medium (Fig. 3A–C). In all cases, only 20–30% of the apoB present at the start of the chase period was degraded. The absolute concentration of apoB turnedover decreased in the order...
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Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc
... as the untagged protein, indicating that the tag was not interfering with the protein activity. The tagged protein was purified and then used for furtheranalysis. In the reductive reaction, the ... in an extractof H. jecorina was about 3 nkatặmg)1. The GLD1 pro-tein was tagged with a histidine tag at the N-terminalend by adding the coding sequence for six histidines to the end of the ... from the pYX212 [17], and the orientation of the open reading frame in the expression vector was checked. The S. cerevisiaestrain CEN.PK2-1B was then transformed with the expres-sion vector and...
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Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... control (141 Torrr O2) and hypoxia (1 Torr O2) for 10 h was carried out using [a-32P]UTP as described in Materials and methods. Rateof32P incorporation in the RNA fraction was determined as ... respiration-coupled ATP synthesis was inhibited in cells grown under hypoxic conditions but the degree of inhibition was dependent on cell type. In macrophages, there was a 22% reduction in ATP synthesiscompared ... was allowed to proceed for 45 min at33 °C. Aliquots were used to determine the level of32Pincorporation in to RNA as described before [19]. At the end of incubation, the mitochondria were pelleted...
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Tài liệu The Economics Associated with Outdoor Recreation, Natural Resources Conservation and Historic Preservation in the United States pdf
... outdoor recreations, including bicycling, camping, fishing, hunting, paddling, snow sports, trail use and wildlife viewing. Unfortunately there are other outdoor sports that are not included ... of information available on the economic impact of various forms of outdoor recreation in the country, including hunting, fishing, wildlife viewing and non-motorized outdoor recreation (hiking, ... and regions across the United States echoing similar findings about the relevant affluence of historic visitors and the far-reaching effects of their visits on local or statewide economies,...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... the binding of LBR to chromatin. It was shown that the binding of LBR fragments to chromatin was stimulated by phosphorylation in the arginine-serine repeat-containing region by a protein kinase(s) ... that the cell cycle-dependent binding ofLBR to chromatin is regulated by phosphorylation in the arginine-serine repeat-containing region b y multiple kinases.Keywords: chromatin binding; lamin ... chromatin in eggs, binding through the RS region ofLBR to linker DNA of chromatin s eems to b e predominant.Then, the binding is supported by the interaction of the NMregion and a protein(s ) other...
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Report on the FAO Workshop on Sea Cucumber Fisheries An Ecosystem Approach to Management in the Pacific (SCEAM Pacific)
... believe they need more support in this area. There will be a COFI meeting in March 2012. In the last COFI meeting, there was no mention of sea cucumbers, which makes it harder to gain funding for ... the workshop and in the post-workshop report. Scope The workshop concerned only sea cucumber fisheries. The focal region for the workshop was the Western-Central Pacific islands (excluding ... already in place. There is an enforcement officer in each town, in charge of enforcing regulations. There are many important research and information needs in the Tonga sea cucumber fishery. In...
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Making the Poor Pay for Public Goods via Microfinance Economic and Political Pitfalls in the Case of Water and Sanitation docx
... gains, medical savings, time gains and how they are spent, minus the total construction costs including interest.24What has emerged more clearly from the findings in Vietnam and India is the ... take the opportunity were in fact building storage tanks on their property at additional cost for collecting excess water from their tap whenever it was delivered. The costs for the storage ... 2003: Banker to the Poor: Micro-Lending and the Battle against World Poverty. New York: PublicAffairs. Mader: Making the Poor Pay for Public Goods via Microfinance 33areas. Given the insight that...
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Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt
... and in J774 cells in both these conditions [25–27]. In other studies, however, no effect on the induction of foamcell formation by acLDL or oxLDL in the presence ofprobucol or vitamin E was ... to the medium either in its free form or in acLDL prepared from patients given the drug, and vitamin E has also been reported to decrease the induction of lipid accumulation by oxLDL or acLDL in HMDM ... transport from the gut to the liver in chylomicron remnants they promote foam cell formation,and their beneficial effects are only apparent after theirincorporation into LDL, as oxidation of these...
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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue-specific expression doc
... the posteriorhindbrain and anterior spinal cord in which the signal was restricted to the dorsal spinal cord and graduallydiminished along the length of the spinal cord(Fig. 5B,F). The hybridization ... cellular retinoid-binding proteins with dis-tinctive binding properties have been identified, the cellular retinol-binding proteins (CRBPs) and the cel-lular retinoic acid-binding proteins (CRABPs). ... andother fish and mammalian cellular retinoid-binding proteingenes was performed using clustalx [53]. A bootstrapneighbor-joining phylogenetic tree was constructed using the zebrafish intestinal-type...
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Báo cáo " The wastewater quality from several industrial production branches and traditional production villages in the Day-Nhue river basin, North Vietnam " ppt
... basin), and about 8% from the Ha Nam province; 7% from the Nam Dinh, 7% from the Ninh Binh province and 2% from the Hoa Binh province. The wastewater quality of six industrial branches in ... locate in the Day-Nhue river basin, for information. Parallel with the documentation process, the wastewater samplings were conducted from April to December 2007 in Hanoi, Ha Nam, Hoa Binh ... basin 3.1.1. Development of industrial factories: Along with the agricultural development, the manufacturing industry in the Day-Nhue basin in recent years has rapidly increased; both in...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... 102CFU for the PMM56 strain. A competitionindex (CI) was determined by calculating the ratio ofmutant to wild-type bacteria after correcting for the ratio of these strains in the inoculum. ... deciphering the complexbiosynthetic pathways of DIMs and PGLs. Theseefforts have led to the identification of more than 20proteins required either for the formation or for the translocation of these ... mice, indicating that fewer than 50 bacteria of the PMM56 mutant strain were present in the spleen of these mice. For the determination of the number of PMM56 bacteria present in the spleen of these...
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Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
... mixture was incubated at 37 °Cfor90min.Gel analysis of the integration reactionFor gel analysis of the integration reaction, the DNA donor was radiolabelled by including 8 lCi [32P]dCTP[aP] in the PCR ... FranceIntegrase (IN) is the retroviral enzyme responsible for the integration of the DNA copy of the retroviral genome into the host cell DNA. The C-terminal domain of IN is involved in DNA binding and enzyme ... concertedDNA integration by bending the donor DNA and helpingto bring the two ends into close proximity; alternatively, the unwinding activity of HMG proteins could facilitatebinding of IN proteins...
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Possible Strategies for Listening Comprehension: Applying the Concepts of Conversational Implicature and Adjacency Pairs to Understand Speaker Intention in the TOEFL Listening Section doc
... choosing the correct answers in the TOEFL Listening Section. The results indicate that the feasibility of the training was mostly evidenced. As for the helpfulness of the strategies, though the ... Oxford’ model of eight steps in the strategy training and the process of organizing and implementing the three lessons following these steps. Oxford’s Eight Steps Lessons for Strategy Training ... called performatives or performatories. Some performatives have, according to Austin, the grammatical make up of statements on the face of them, but are distinct from statements in that they are...
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Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot
... the substratebinding site and the role of the metal cofactor in the enzyme.ResultsCloning, expression and purification of BphH The bphH gene was inserted into the expression vectorspT7-7 [11], ... rather than just a substrate binding role. Possibleroles of the cation include the activation of water for the hydration reaction and ⁄ or the stabilization of the anion transition state. In the ... constructedby inserting the previously cloned gene [25] into the NdeIand HindIII sites of the expression vector pET28a. The bphH gene was amplified by PCR using the primers with the respective...
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