... decrease in the intrinsic fluorescence of GST–HIPPI protein, indica-ting binding ofthe protein with this mutated motif(Fig. 1B, panel II). The apparent dissociation constant(Kd) of this binding ... protein; GST, glutathione S-transferase;HD, Huntington’s disease; HIP1, Huntingtin interacting protein 1; HIPPI, Huntingtin interacting protein 1 protein interactor; Htt, Huntingtin;IOD, integrated ... further confirmed thein vitro resultthat mutation ofthe motif abolished the binding of HIPPI to the specific sequence ofthe caspase-1 geneupstream sequence, and the increased expression of caspase-1...
... the information obtained about the 3D struc-ture of CDase I-5, the quaternary state of CDase I-5was likely to be maintained by the intrinsic capability of the N- and C-terminal regions ofthe ... data indicated that the effect of pH on dissociation ofthe oligomer was mainlydue to the protonation of a single residue rather than aglobal effect of pH on the protein. In agreement with the ... inthe C-terminaldomain at low pHs. Thus, H539 that is inthe center of the C-terminal region plays an important role in determining the quaternary structure ofthe dode-camer. Four histidine...
... about the role of these factors in vivo and their role in furunculosis. The A-layer is believed t o play a crucial role in the bacterial protection against c omplement-mediatedkilling in vitro ... pectrometer. The extent of amidation could be determined by the intensity ratio of fragments at m/z 662.2 D a (GalNAcAN-containing trisac-charide repeating unit) and m/z 663.2 Da (GalNAcA-containing ... sample of A. salmonicida (data not shown). The proposed structure of CPS and O-chain polysaccharide of A. salmonicida strain 80204-1 is depicted in Fig. 5. The biological significance of these findings...
... sauvagine could be observed in the presence of any of these inhibitors. These findingsindicate that neither the activation of PKA nor the activation of PKC is necessary for agonist-induced CRFR1internalization.Involvement ... internalization of radioligand wasobserved during the initial 10 min and the maximal extent of internalization was reduced to 39% following 40 min of incubation (Fig. 2). This result indicate s the involvement ... receptoractivation even inthe absence ofthe C-terminal tail of the receptor and the cluster of serine/threonine inthe thirdintracellular loop. This could be due to heterologousrecruitment to other endogenously...
... including limp(5 points), support (5 points), locking (15 points), insta-bility (25 points), pain (25 points), swelling (10 points),stair climbing (10 points) and squatting (5 points).Instrumented ... increase in osteoar-thritis of 1 grade in 52% ofthe patients, 35% of the patients had an increase of 2 grades and 3% ofthe patientshad an increase of 3 grades. (Table 4, 5 and 6) 10 % of the patients ... OA. The major factor with the potential todiminish this risk is improvement and maintenance of joint stability, resulting in a lower frequency of repeatinjuries, especially ofthe meniscus. In...
... Einstein College of Medicine of Yeshiva University, Bronx, New York, USA.Authors’ contributionsBAM assisted inthe organization/administration ofthe study, gathering of the data, analysis of ... (FACT-Bl). These instruments were administered at differenttime points after surgery, usually in a single mailing or asingle session inthe clinic after radical cystectomy. Onlyone ofthe studies included ... incl uded examination of patien t character-istics and quality of life, thematic content coding of responses to the Brief Quality of Life Appraisal Profile,examination ofthe relationship of...
... but may also influence the rate of cooking and optimum boiling time. One approach in determining optimum boiling time is to judge the time when the uncooked central core ofthe noodle strands ... thickness of noodles after boiling varies according to boiling time and the degree of starch swelling (Crosbie 1992). Accurate measurement ofthe color of boiled noodles necessitates the use of a ... descriptor of lightness. The official Japanese method for the assessment of udon noodles incorporates visual sensory testing ofthe boiled noodles for color (including brightness, glossiness,...
... operating surgeons inthe whole series and criticallyreviewed the paper. Dr RS, Dr KB and Dr AKM maintained all the records of the patients and followed them. All the authors read and approved the ... reconstruction was the index surgery in 10 of the 12 cases and rest 2 were cases of recurrent GCTinitially treated with extended curettage and bonecementing for these grade II tumors. Ofthe 10 primarycases, ... raduallyFigure 2 Clinical picture showing the biopsy scar and incisionoutline highlighting how the biopsy tract has to be carefullyexcised in this patient.Figure 3 Intraoperati ve picture showing the fixation...
... operating surgeons inthe whole series and criticallyreviewed the paper. Dr RS, Dr KB and Dr AKM maintained all the records of the patients and followed them. All the authors read and approved the ... raduallyFigure 2 Clinical picture showing the biopsy scar and incisionoutline highlighting how the biopsy tract has to be carefullyexcised in this patient.Figure 3 Intraoperati ve picture showing the fixation ... reconstruction was the index surgery in 10 of the 12 cases and rest 2 were cases of recurrent GCTinitially treated with extended curettage and bonecementing for these grade II tumors. Ofthe 10 primarycases,...