... superimpo-sition of five-residue segments ofthe crystal structure on the equivalent segments of each member ofthe family ofthe solution structure ofthe M domain of human eRF1. The resulting rmsd ... S4. A comparison of part ofthe protein backbone structure ofthe representative solution structureof the human eRF1 M domain andthe Ca trace in the crystal structureof RF1 in the whole ribosome ... simi-larity to the crystal structureofthe M domain of the same protein [3], but it is far from identical (Fig. 2A). The rmsd ofthe superposition ofthe heavy backboneatoms (Ca, N, O and C) ofthe family...
... between the Leu residues from v-KIND and MAP2 contributes to the interaction between the two proteins.In conclusion, the present study has clarified the structural and functional importance ofthe ... and 482) and one Thr residue (amino acid 487) were well conservedin the KIND2 of v-KIND in all species analyzed,although not in the other KINDs. To investigate the possible involvement of these ... under-stood, the data obtained in the present study indicatethat the 43 residues (amino acids 702–744 in mice) thatreside in the middle region ofthe CD act as the v-KIND binding core. The v-KIND-binding...
... foreign lands, were not confined within the shores of a little island. Most ofthe islands of a continent, and many of these of considerable population and extent, were filled with them. Andthe ... liberty, and make them slaves and sell them, are the worst of robbers, and ought to be considered as the common enemies of mankind; and that they who buy them, and use them as mere beasts for their ... nature of it; andofthe extent of it; and ofthe difficulty of subduing it.—Usefulness also ofthe contemplation of this subject. I scarcely know of any subject, the contemplation of which...
... correct, and just way of viewing the law. Law and government should serve the common good ofthe people. The commongood ofthe people will be found best through the checks and balances of the ... Legal Theory The History, Constitution and Purposes of Law in a Free State M.N.S. SellersRegents Professor ofthe University System of Maryland and Director ofthe Center for International and ... of the public good.19 The rule of law The third traditional desideratum of republican government requires the rule of law ( the empire of laws and not of men”), which protects justice and the...
... SDS/PAGE and in 2D PAGE (Figs 1 and 2; Table 1). The N-terminal amino acid sequences (see above) allow the assignment ofthe HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... against the hemocyanin fraction showstaining ofthe two 75 kDa bands in Western blot, but donot recognize any other proteins ofthe hemolymph.Although we expected that each of these bands containstwo ... primers andthe T3 vector primer. The sequences were obtained after the cloning ofthe PCRproducts into the pCR4-TOPO TA vector (Invitrogen).Protein structure modelingHomology models ofthe S....
... us. Neither of theseparameters are within the range of our experimentalobservations, and therefore the predominant species insolution are the monomer and dimer. Additional evidencethat the crystallographic ... arrestin and arrestin in the presence of phosphopeptide is given at the top ofthe (B) and (C), expressed as apercentage ofthe total signal from the detector.Ó FEBS 2002 Activation of visual ... provide the radius of gyration (Rg), definedas the root mean square distance of all atoms from theircommon centre of mass. The Rgcanbeusedtoevaluatetheoverall shape of a scattering particle. The...
... region of the 1H,15N-HSQC spectrum of the C-terminal domain of human eRF1 illustrating the presence of two conformational states ofthe protein.Fig. S2. Plot ofthe number and distribution of the NOEs ... However, thesestructures do not show the coordinates ofthe atoms inFig. 6. Superposition ofthe representative NMR open conformer of the C-domain (red and blue) on the crystal structure [20] of the complex ... (C-domain). The high-reso-lution NMR structureofthe human C-domain (residues 277–437) has beendetermined in solution. The overall fold andthestructureofthe b-strandcore ofthe protein...
... with the gene ofthe benzaldehyde lyase and for helpful discus-sions, the teams ofthe Swiss Light Source (Villigen ⁄ CH) and ofthe EMBL outstation Hamburg for their helpwith data collection and ... comparison ofthe relativeB-factors along the ThDP molecules shows that the Fig. 4. Structure- based sequence alignment of BAL, BFD and PDC all of which are used in organic synthesis. The secondary structure ... consist of a cen-tral six-stranded parallel b-sheet flanked by a varyingnumber of a-helices. Residues involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of...
... parts of a leaf are the tip of the blade andthe top ofthe sheath. Where the blade and sheath join, there are structures called the ligule and the auricles (Figure 2.3). Table 2.1 – Shoot structures ... EFFICIENCYTim Setter and Peter Carlton The structureofthe wheat plant described in thischapter is the starting point to understanding the growth and development ofthe crop, its nutrition andthe reasonsfor ... determines the rate of uptake.Uptake of nutrient elements from the soil and intoplants depends on: the availability ofthe nutrient in the soil;movement ofthe nutrient, in soil solution or along the surfaces...
... in the presence and absence ofthe paramagnetic metal ions. The amplitudes of the spectra in the presence ofthe paramagnetic metal ionswere normalized to the least affected cross-peaks. The paramagnetic ... biology aspects of DMT1 since the discovery of this gene, there has so far been no structuralcharacterization of either this integral protein or a segment of it. Analysis ofthestructureof membrane ... in TFE and SDS.Ó FEBS 2004 Structureand topology of TM4 of DMT1 (Eur. J. Biochem. 271) 1951peaks of Ile10, Ile12 and Val17. This suggested that the peptide was inserted in the interior of micelles....
... The structure images were prepared using the MOLMOLprogram [49].ResultsExpression and purification ofthe Xis_wt and Xis_C28S proteins The isolation and purification of Xis was hampered by the fact ... to otherproteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... the N-terminal, structured part of Xis is involved in the recognition of DNA [18,57]. On the other hand, the resultsalso indicate that the C-terminal a-helix, predicted in the work of Wu and co-authors for...
... highlytwisted as a result ofthe conserved flexible Gly5followed by the bulky side chain of lysine and the disulfide-paired Cys7, which pulls the strand towards Structure and dynamics of an antifungal ... 10. The electrostatic surface potential of AFP (left) and PAF (right) structures representing the orientation ofthe side chain lysine resi-dues. Lys9, Lys35 and Lys38 of PAF and Lys9, Lys32 and ... anyredox activity of PAF.Differences and similarities between the structures of PAF and AFP The common geometrical arrangement ofthe twoproteins is the special Greek key fold, in which the two orthogonally...
... Additionally,because the demand ofthe chloroplast is ‘sensed’inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complexcould be one ofthe fastest ... transmembrane-helices at the extreme N-terminus, which anchor the protein in the membrane. The position and function ofthe longC-terminal tail on the other hand remains a matter of controversy [10,11,13–15]. ... description of recent advances in the understanding ofthe chan-nel-, motor- and regulatory components ofthe Ticcomplex. For reference, some ofthe available know-ledge, including the proposed...
... paradigm, on the other hand, is characterised by a kind of “postmodern” mentality (Rodowick 75). In it, the faltering belief in totality, either from the point of view ofthe grand “organic” ... to the subject as (the myth of) a coherent and autonomous agency, leads to the disintegration ofthe action-image (75-76). The old alignment of space and time is here dislodged, while the ... here dislodged, while the break ofthe traditional links of cause and affect leads to a new breed of signs wherein the “power ofthe false” andthe principle of “indiscernibility” become predominant...