rnai technology to block the expression of molecules relevant to metastasis the cell adhesion molecule ceacam1 as an instructive example
Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... blot analysis and (B) western blot analysis (A) Northern blot analysis Each lane contains 15 lg total RNA The bottom panel shows the expression of 18S rRNA as an internal control Note that the ... mRNA and protein and induced the expression of HO-1 mRNA and protein In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins in HeLa and HepG2 cells ... Results Expression profiles of HO-1 and HO-2 in various human cell types We initially analyzed the expression profiles of HO-1 and HO-2 in eight human cell lines by northern and western blot analyses...
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Effects of high glucose concentrations on the expression of genes involved in proliferation and cell fate specification of mouse embryonic neural stem cells
... case, the brain development ceases and the vault of the skull fails to form Failure of the caudal neuropore to close leads to spina bifida The severity of this case depends on how much of the spinal ... plate with the lateral ectoderm The cells of these two regions are called the dorsolateral hinge point (DLHP) cells Each of the three hinges acts as a pivot to direct the rotation of the cells around ... promote the differentiation of autonomic neurons and induce the expression of Ascl1 indicating that BMP2 and BMP4 control the specification of autonomic neurons through the induction of Ascl1 expression...
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Báo cáo khoa học: Therapeutic targeting of molecules involved in leukocyte–endothelial cell interactions potx
... stages of the multistep recruitment cascade of leukocytes The function of inflammatory adhesion molecules can be modulated by competitive blockade, altered surface expression of ligands and adhesion ... perpetuation of inflammatory diseases Activation of chemokine receptors by their ligands leads to the activation of integrins, resulting in firm adhesion to the endothelium Therefore, for many years, ... ligands, such as P-selectin glycoprotein ligand-1 (PSGL-1) [12] P-selectin is stored in granules of endothelial cells and platelets and it translocates to the cell surface following exposure to...
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Báo cáo y học: "Insulin augments tumor necrosis factor-alpha stimulated expression of vascular cell adhesion molecule-1 in vascular endothelial cells." pot
... characteristic of atherogenesis is the increased expression of cellular adhesion molecules (CAMs) at the surface of vascular endothelial cells [2-4] Although insulin is considered to be an anti-atherogenic ... increased expression of cellular adhesion molecules on the surface of vascular endothelial cells VCAM-1 is one such adhesion molecule that is upregulated in the state of insulin resistance and ... surface adhesion markers, which in turn are recognized by cellular adhesion molecules that are located on the surface of the endothelial cells of the intima Activated immune cells translocate into the...
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Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
... residues The palmitoylation serves as a second anchor to the plasma membrane and is possibly organizing the remaining cytoplasmatic tail for the interactions with other molecules that are important ... microfilaments) and intracellular signaling molecules, integrins serve as a linker between the extracellular and intracellular environments Ligand binding leads to signal transmission into the cell ... between NCAM molecules expressed on neighboring cells) as well as cis-binding, i.e the binding between NCAM molecules or between NCAM and other molecules on the same cell surface leading to the inhibition...
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Báo cáo khoa học: Anti- and pro-oxidant effects of quercetin in copper-induced low density lipoprotein oxidation Quercetin as an effective antioxidant against pro-oxidant effects of urate potx
... [42] To this end, 0.25 mL of water, mL of ethanol and mL of hexane were added to 0.25 mL of LDL The hexane upper phase (2 mL) was collected and the visible absorption spectrum (350–600 nm) was ... lipophilic and aqueous phases, their binding to other biomolecules and their interaction with other antioxidants Quercetin was chosen as a representative flavonoid in this study as it is rather universally ... antioxidant Antioxidant properties of flavonoids have been widely studied within the last 20 past years A peculiar attention has been paid to the antioxidant effect of flavonoids towards the oxidation...
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báo cáo khoa học: " Early activation of wheat polyamine biosynthesis during Fusarium head blight implicates putrescine as an inducer of trichothecene mycotoxin production" potx
... tenuis led to decreased levels of polyamines [28] Increasing polyamine levels via overexpression of polyamine biosynthetic enzymes has been shown to increase the tolerance of tobacco to F oxysporum ... Importantly, trichothecenes such as DON are toxic to animals and humans when present in feed and food products, respectively [6] Because of the undesirable effects of DON on human and animal ... genes for their biosynthesis in relation to the biosynthesis of the trichothecene DON during FHB disease development A key finding was that the induction of expression of key polyamine biosynthetic...
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AN0566 using the PORTB interrupt on change as an external interrupt
... in theft of intellectual property Microchip is willing to work with the customer who is concerned about the integrity of their code Neither Microchip nor any other semiconductor manufacturer can ... any other semiconductor manufacturer can guarantee the security of their code Code protection does not mean that we are guaranteeing the product as “unbreakable” Code protection is constantly ... AN5 66 Figure shows the two cases for the interrupt signal verses the time to complete the interrupt service routine The first waveform is when the signal makes the low -to- high -to- low transitions...
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20 quotes to ring in a successful new year as an entrepreneur
... aptitude for adventurous business risks, creating inventive ideas, filling a gap in consumer needs, Whether you are an aspiring or seasoned ...
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Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"
... In cases where an ESC has successfully contributed to the embryo, the resultant offspring will be chimeric (up to ca 50% of animals) The ESC contribution to a mouse can high (up to ca 80% of the ... majority of these reports provide evidence of post-fertilisation transfer and maintenance of transgenes Several of the studies report the subsequent generation of viable progeny animals, the cells of ... costly and technically demanding, due in large part to the need to remove embryos from the female, to manipulate the embryos and finally to return the embryos to the reproductive tract By contrast,...
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The research of using epad technology to support activities in administrative system
... successful: • The technology used: it decides the effect of the software including powerful, fast, etc or the compatibility with other softwares or browsers • The cost of the software: the cheaper software ... the fact that hundreds of task given every day suggests each person have a schedule to remind next tasks and to arrange time logically The purpose of the system is to change all four issues of ... level of staff member, which decided by the manager • The destination of the documents after reviewing depends on the manager (the manager will note on the document to which department the documents...
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Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc
... Glattbrugg, Switzerland) Membrane protein fractions (in the case of COX I, COX Vb and F1-ATPase) and total muscle extracts (in the case of TnIfast and cytochrome c) were used for quantification Proteins ... enzymes, on the one hand, and mitochondrial biogenesis, on the other Renewed interest in mitochondria was brought about by the recognition of their role in apoptosis [30], Ca2+ homeostasis [31] and signal ... delay of 20 ms as compared to rises in [Ca2+]i, and peak [Ca2+]m was reached during the relaxation phase Thus, the effect of mitochondria on [Ca2+]i is very similar to the role of PV (i.e to promote...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... whether iron loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class ... class I molecule localizes in the cytoplasm of human epithelial cells of the gastrointestinal tract and liver, two central organs in the regulation of iron metabolism [20,21] After their synthesis ... inhibition of cell growth was due to a decrease in the percentage of cells in the S and G2 ⁄ M phases of the cell cycle (Fig 3B) In a total of seven separate determinations, a statistically significant...
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Báo cáo khoa học: Sp1 binds to the external promoter of the p73 gene and induces the expression of TAp73c in lung cancer doc
... fibroblast cell lines (CCL171 and IMR90), and substantially increased in the lung epithelial anaplastic carcinoma cell line (CALU6), the small cell carcinoma cell line (DMS53), the squamous lung cancer ... promoter analysis, as follows The direction of transcription of tp73 was identified, and the most upstream TSS of all tp73 Ensembl [45] transcripts was selected One thousand base pairs of the UCSC ... carcinoma cell line (Fig 2C, lane 3), and the levels of the complexes appeared to remain equivalently high in the small cell lung cancer cell line (Fig 2C, lane 4), the squamous cell carcinoma cell...
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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx
... Effects of expression of muscle glycogen phosphorylase To test whether a higher activity of phosphorylase can account for the lower rate of glycogen synthesis in fa ⁄ fa hepatocytes we expressed the ... activities of phosphorylase-a [11,12] and glycogen synthase phosphatase [13–15] However, the total activity of glycogen synthase and the activation state are the same as in control hepatocytes [6,11] ... function from the other targeting proteins Based on assays of phosphorylase phosphatase and glycogen synthase phosphatase in both the glycogen fraction and the soluble fraction, R6 appears to have...
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Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf
... human cells and the mechanisms of regulation of the PRDX5 gene are not established In mammalian cells, the transcriptional response to antioxidants and electrophiles is governed by promoter-associated ... antioxidant protein, peroxynitrite reductase [13,14] and an inhibitor of p53dependent apoptosis [15] Antiapoptotic activity of the human recombinant PRDX5 gene has also been detected in transfected ... stable clones of A549 cells (Control and KD-1, lanes and 2; their isolation is described in the legend to Fig 5), and A549 cells transiently transfected with plasmid (lane 3, 48 h after transfection)...
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USE OF BIOMETRIC IDENTIFICATION TECHNOLOGY TO REDUCE FRAUD IN THE FOOD STAMP PROGRAM: FINAL REPORT docx
... visits to offices of the Texas Department of Human Services in Austin, as well as to the demonstration offices in San Antonio, in December 1996 and June 1997 The purpose of the visits was to observe ... staff to describe the planned system, the activities surrounding the implementation of the system, the needs and expectations of the system, and to provide the counties with the opportunity to ask ... prior to release of the held benefits Mandatory Participants and Exemptions All adults (over the age of 18) applying for food stamp, TANF, and State general assistance benefits must submit to the...
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báo cáo hóa học:" Bioactivity-guided identification and cell signaling technology to delineate the immunomodulatory effects of Panax ginseng on human promonocytic U937 cells" potx
... release from rat peritoneal mast cells and the IgE-mediated passive cutaneous anaphylaxis reaction in mice [12] Rh1 and 20(S)-Protopanaxatriol inhibit the LPS-induced expression of iNOS and cyclooxygenase-2 ... slices of to mm, and then placed in a flask that was heated with 70% ethanol-water under reflux for hours The experiment was repeated twice The ratio of the plant material to the menstruum was 1:10 ... the cytotoxic effects of PGSE at mg/ml by trypan blue exclusion assay The viability of cells was over 90% after incubating U937 cells with the PGSE for 48 hours Cytotoxicity test of PGSE Cytotoxic...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
... On the other hand, the VP16 activation domain mechanism of transactivation is the result of interactions with a variety of factors including histone acetylases, basal transcription factors, and ... Ovarian Kaleidoscope Database (OKdb) to determine if other investigators had published evidence of ovarian expression of these genes A total of 71 of the genes were found to be expressed in the ... a total of 42 genes common to both (Additional file 10) In order to begin to understand the significance of the genes common to the three microarray studies in the ovary, we then searched the...
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