phân tích gói tin bằng wireshark

Functional studies of a type III and a novel secretion system of edwardsiella tarda

... extra-intestinal infections Gastro-intestinal infections are common compared to extra-intestinal infections Although infections caused by E tarda in humans are uncommon, gastro-intestinal infections ... Sperandio et al., 2000; Sanchez-SanMartin et al., 2001) by competing for binding with HNS, which represses their expression in the absence of Ler (Sanchez-SanMartin et al., 2001; Haack et al., 2003) ... were tested for adherence showed both mannoseresistant and mannose-sensitive hemagglutination The hemagglutination activity may be mediated by non-fimbrial proteinaceous adhesions, as E tarda...

plasmids and vectors - Instructor Supplement to pGlo Bacterial Transformation

Danh mục: Sinh học

... (selects for recombinant molecule) EcoRI TetR pBR322 4,361 bp BamHI Screening bacteria by replica plating Next Major Advance in Plasmid(ology)    The inclusion of polylinkers into plasmid vectors ... DNA size is 12 kb  Requires the preparation of “competent” host cells  Inefficient for generating genomic libraries as overlapping regions needed to place in proper sequence Preference for...

Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

Danh mục: Báo cáo khoa học

... representing the R3 core type Eur J Biochem 203, 127–134 Haishima, Y., Holst, O & Brade, H (1992) Structural investigation on the lipopolysaccharide of Escherichia coli rough mutant F653 representing ... Brade, H., Brade, L & Peters, T (1999) NMR experiments reveal distinct antibody-bound conformations of a synthetic disaccharide representing a general structural element of bacterial lipopolysaccharide ... and 4) in 0.5 mL solutions in D2O using a Bruker DRX 600 Avance spectrometer equipped with a multinuclear probehead with z-gradient Acetone served as a reference 2.225 p.p.m (1H) and 31.5 p.p.m...

Maltose and maltotriose transport into ale and lager brewer´s yeast strains docx

Danh mục: Cao đẳng - Đại học

... (Piddocke et al., 2009) 1.1 Outline of malting and brewing processes A schematic diagram of malting and brewing processes is presented in Figure Malt is the main starting material in the brewing process ... material, starch, composed mainly of amylose and amylopectin, to fermentable sugars Degradation of starch starts during malting and continues at wort production phase In wort production, malt ... VTT, Finland phone internat +358 20 722 111, fax + 358 20 722 4374 Technical editing Mirjami Pullinen Text formatting Raija Sahlstedt Edita Prima Oy, Helsinki 2010 Virve Vidgren Maltose and maltotriose...

Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx

Danh mục: Hóa học - Dầu khí

... protein Serp2-GFP Resting PBMC were infected with T1-Serp2-GFP or SG33GFP at a m.o.i of (Figure 3A) Cells were collected 16 h p.i., and infection levels were determined by counting living GFP-positive ... vaccination http://www.virologyj.com/content/4/1/94 10 11 12 13 Competing interests The author(s) declare that they have no competing interests 14 15 Authors' contributions BP conducted all the experiments, ... (AFSSA) and ANR Génanimal 2006 "VacGenDC project" The authors are especially grateful to Martine Deplanche, Martine Moulignié, Brigitte Peralta and Josyane Loupias for excellent technical assistance,...

Báo cáo hóa học: " Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains" potx

Danh mục: Hóa học - Dầu khí

... GenBank number: U26362) that showed three out of the four nucleotide mismatches (Fig 1) Interestingly, when we compared the 173 sequences of G1 strains with the aBT1 primer binding site, we found ... found that 170 strains showed one or two nucleotide mismatches and showed three mismatches, suggesting that Gouvea's RT-PCR strategy could type these strains correctly (data not shown) Page of (page ... replication In Fields virology 4th edition Edited by: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B and Straus SE Philadelphia, Pa, Lippincott/Williams & Wilkins; 2001:1747-1785...

Screening of Bacillus strains as potential probiotics and subsequent conﬁrmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs doc

Danh mục: Ngư nghiệp

... dilution in physiological saline Simulated small intestinal fluid tolerance test Simulated small intestinal fluid was prepared by suspending pancreatin (porcine pancreas, Sigma) in Mixed Nutrient broth ... consisting of the basal diet with no additives (Group C), a positive control consisting of the basal diet supplemented with 16 g/ ton flavomycin (Group A) and three experimental diets consisting ... intestinal fluid on spore viability Another barrier that probiotic bacteria must survive is passage through the small intestine (Huang and Adams 2004) The experimental results of Casula and Cutting...

Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot

Danh mục: Ngư nghiệp

... in journal articles Gene 23, 245–252 (2000) KURMAN J.A.: Starters with selected intestinal bacteria Bulletin IDF 227, 41–55 (1988) LJUNGH A., WADSTROM T.: Lactic acid bacteria as probiotics Curr.Issues ... also established in colon and cecum contents Results were expressed as the number of CFU/g of intestine contents or the number of CFU per organ or pooled MLN from each group Lymphocyte cultivation ... sequence D79212 (L paracasei) 536 B CUKROWSKA et al Vol 54 Bacteria translocation through the intestinal barrier The number of Lactobacillus CFU in colon (3.3 ± 0.5 × 109) and cecum (2.3 ± 0.2 ×...

Báo cáo khoa học: "Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen" docx

Danh mục: Báo cáo khoa học

... and the autoagglutination in the presence of low concentrations of sodium hydroxide Although the addition of milk whey in some strains was not effective in promoting autoagglutination, a significant ... mixture was incubated for h at 37oC After determination of agglutination, the mixture was allowed to stand overnight at 4oC When agglutination titers were 640 or more, the rabbit was exsanguinated ... Puteaux, France) SDS-PAGE and western blotting CP was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using the discontinuing system of Laemmli [22] CP was separated...

Báo cáo khoa học: "Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains" pps

Danh mục: Báo cáo khoa học

... ecneuqes htiw nosirapmoc rof dettimbus saw etalosi naisenodnI eht fo 1GAS erutam fo ecneuqes eht ,noitinifed niarts ni esu elbisop sti dna ,noitavresnoc fo eerged sti etagitsevni ot redro nI eert citenegolyhp ... siserohportcele yb dezylana neht erew stcudorp noitsegiD nim 03 h rof Co73 ta ,emyzne hcae fo stinu ,noitulos dimsalp lµ 0.5-5.2 gniniatnoc reffub lµ 02 ni ,IRocE -IHmaB htiw noitsegid elbuod ... Co06 ta gnilaenna ,ces 04 rof Co59 ta noitarutaned fo selcyc 03 ;nim rof Co49 ta noitarutaned laitini :snoitidnoc gniwollof eht ta ,)ANDc ro AND cimoneg( xirtam fo stnuoma elbairav dna ,remirp...

Báo cáo sinh học: "Molecular epidemiology of clinical and carrier strains of methicillin resistant Staphylococcus aureus (MRSA) in the hospital settings of north India" pptx

Danh mục: Báo cáo khoa học

... Medical College, Aligarh Muslim University, Aligarh India, contributing nasal swabs besides pus culture and 525 subjects contributing different clinical sources were included Informed consent was ... patterns are indistinguishable between MRSA from the nasal cavity (C1) and Pus (C1) and closely related to that of MRSA from the skin (C2) for case (Fig 5) PFGE patterns are indistinguishable between ... indistinguishable between MRSA from the nasal cavity (na) and pus (ps) but the patterns were different for MRSA from the skin (s) and nearby wounds for cases and PFGE patterns were indistinguishable...

Báo cáo sinh học: "n vitro activity effects of combinations of cephalothin, dicloxacillin, imipenem, vancomycin and amikacin against methicillin-resistant Staphylococcus spp. strains" pdf

Danh mục: Báo cáo khoa học

... most of all, in developing countries with a limited formulary Competing interests The author(s) declare that they have no competing interests Authors' contributions MVP drafted the manuscript, ... performed by Mueller-Hinton broth supplemented with 2% of NaCl Resistance was corroborated by detecting the mec A gene by PCR, by the method previously described [13,14] Reference type strains included ... 2006, 5:25 http://www.ann-clinmicrob.com/content/5/1/25 Results of the checkerboard synergy testing are summarized in table For most of the isolates the combination cephalothin plus amikacin...

Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium" ppsx

Danh mục: Báo cáo khoa học

... autoagglutination was normalized to the starting OD ((Starting OD - Final OD)/Final OD × 100) The bars indicate the percentage of cells autoagglutinating Significant differences in autoagglutination ... post-translational modifications of it are required for autoagglutination, which is linked to virulence [48] Significant differences in autoagglutination between 630 and the more recent 027 isolates R20291 ... isolates BI-1 and CD196 show no significant difference in autoagglutination compared to 630 (Figure 5) The differences in autoagglutination observed between 630 and the recent 027 isolates are likely...

Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx

Danh mục: Báo cáo khoa học

... stimulating factor (rhM-CSF), granulocyte-colony stimulating factor (rhM-CSF), interleukin-4 (rhIL-4) and interleukin-2 (rhIL-2) were obtained from Peprotech (Rocky Hill, NJ) Phytohemagglutinin-P ... to receive a vaginal irritation rating of "minimal," scores of to receive a vaginal irritation rating of "mild," scores of to 11 receive a vaginal irritation rating of "borderline," and scores ... with the metal; 3) Separation, purification and lyophilization of the resulting metallic compound; 4) Reaction of the resulting product with ascorbic acid in aqueous solution, and formation of zinc...

Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps

Danh mục: Báo cáo khoa học

... genetic diversity is high and continuing to evolve http://www.aidsrestherapy.com/content/6/1/27 10 11 12 Competing interests The authors declare that they have no competing interests Authors' contributions ... based on V3 sequencing are comparable with the phenotypic method suggesting their potential applicability in clinical settings for the future Another observation in our limited study is that among ... phenotype was R5X4 with VL being 141,198 copies/ml suggesting potential advancement of disease accompanied by X4 tropism and drug resistance Interestingly the virus was a URF composed of the predominant...

Báo cáo khoa hoc:" Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity" potx

Danh mục: Báo cáo khoa học

... inherent charge given to these AuNPs, without adding a special coating that could have a potential cytotoxic effect make these NPs interesting for DNAbinding and cell transfection Previous studies demonstrated ... After transfection, the expression of simple proteins as GFP and the nuclear acting HMGB1 and of complex proteins consisting of two separate subunits as IL-12 is possible Furthermore, the addition ... 8, 30419 Hannover, Germany 4Institute of Veterinary Medicine, University of Goettingen, Burckhardtweg 2, 37077 Goettingen, Germany 5Institute of Technical Chemistry I, University of Duisburg-Essen...

báo cáo khoa học: "Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection" docx

Danh mục: Báo cáo khoa học

... microalgae in Göttingen; SDA: stearidonic acid Acknowledgements and Funding The authors are grateful to Dr Fredi Brühlmann (Geneva) and Dr Cornelia Göbel (Göttingen) for their continuous support ... interpretation of data, Dr Maike Lorenz (Göttingen) for continuous support with microalgal handling, database work and interpretation of data, Prof Dr Rüdiger Hardeland (Göttingen) for providing with dinophyte ... distinction of all three groups SIMPER showed the Rhodophyta/Glaucophyta composite group Figure Discrimination of 162 algal strains of the Plantae supergroup into three subgroups representing...

Báo cáo y học: " Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China" ppt

Danh mục: Báo cáo khoa học

... last boosting and spleen cells were harvested Splenocytes were fused with SP2/0 myeloma cells using 50% (v/v) polyethylene glycol (PEG, Sigma-Aldrich) The resulting hybridomas secreting antibodies ... homologous proteins (Figure 3A), indicating that these proteins are antigenically different Further, the E2 protein of vaccine C-strain is antigenically distinct from those of a wide spectrum of ... manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests Received: 26 October 2010 Accepted: 31 December 2010...

Báo cáo y học: "Genetic diversity and C2-like subgenogroup strains of enterovirus 71, Taiwan, 2008" pptx

Danh mục: Báo cáo khoa học

... consultation and editing of the manuscript HSW revised the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests ... subgenogroup C0 were observed in Japan in 1978 [18,19] One Indian isolate in 2001 was genetically distinct from all other EV-71 strains and designated as genotype D [20] Since EV-71 was first isolated ... 8-years, the incidence of mild cases of HFMD/herpangina was reported as 0.8 to 19.9 cases per sentinel physician per week The seasonal incidence varied, but usually peaked in the summer [30] Over...

Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx

Danh mục: Báo cáo khoa học

... detection and differentiation of wild-type and vaccine strains of CDV Competing interests The authors declare that they have no competing interests Si et al Virology Journal 2010, 7:86 http://www.virologyj.com/content/7/1/86 ... Only one specific band was observed at an anneal temperature from 49.5-54.5°C, with the most distinct band appearing at 51.5°C Thus, 51.5°C was chosen for the RT-nPCR Specificity of the multiplex ... independent inter- and intra-assay replicates of the multiplex RT-nPCR gave consistent results, indicating the repeatability of the assay Figure Amplification of genomes of different easily infected...

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