out of the furnace site

Coming Out of the Dark

... is the direction from which a dominant light originates. The lighter values closer to the light source, are near the top of the sphere. The tiny glow on the lower edge of the circle is the ... the outward outline of a form. Basic shapes include circles, squares and triangles. The light source is coming from the upper left, which affects the placement and value of every section of ... away values rather than adding them! ILLUSTRATION 02-04 1) Shade in a section of your drawing space with your stick of charcoal. Use the side of your charcoal instead of the end. It’s...

How to get out of the friendzone: turn your friendship into a relationship

Danh mục: Tâm lý - Nghệ thuật sống

... for him? 16 How to Get Out of the Friend Zonewould hang out with them until the wee hours of the morning, watching them party until they inevitably hooked up with one of my many skinny blonde ... order all of Kaylie’s previous boyfriends. But they never talk about the two of them dating. It’s the ultimate taboo subject. He thinks about it all the time, sure. But they never talk about it.Ugh, ... calls out of the blue to see if you can go yard saling with them, the thought of saying no makes a little part of your heart die. You want them to love you so badly that it’s hard to breathe....

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Tài liệu COMING OUT OF THE FOODSHED: CHANGE AND INNOVATION IN RURAL ALASKAN FOOD SYSTEMS pptx

Danh mục: Cao đẳng - Đại học

... example; the third, from which the title of this thesis was taken, presents the process and outcomes of contemporary food system change for the Athabascan village of Minto, AK, as they “come out of ... information about the services they offer to their customers, how and where these services are provided, and the policies that govern their use. Chapter 2 presents a modified version of the SOA as ... lay out the prototype of the SOA as a way to further the usefulness of the ecosystem services framework and demonstrate it using an example from rural Alaska. This chapter offers a set of common...

Tài liệu Out of the Shadows African American Baseball from the Cuban Giants to Jackie Robinson ppt

Danh mục: Du lịch

... citing the achievements and exploits, on and off the ﬁeld, of the so-called weaker sex. BOB — University of Nebraska Press / Page 2 / / Out of the Shadows / Bill Kirwin2 The Birth of the Cuban ... was good, for the owners both of the Negro team and the local team. The attendance for the annual game wasoften the highest of the year.✶Parallel with serious Negro ball were the black clown ... IntroductionJerry Malloy 1 The Birth of the Cuban Giants: The Origins of Black Professional BaseballLee Lowenﬁsh 15 When All Heaven Rejoiced: BranchRickey and the Origins of the Breaking of the Color Line...

Uncommon Sense: Out of the Box Thinking for An In the Box World

Danh mục: Quản trị kinh doanh

... control. They get the customer in the car and demon-strate the acceleration, the road holding and the hi-ﬁ . In short, they impress the heck out of the customer and if they should ask a question, then ... then they show them the engine compartment and other features.When was the last time you looked under the hood of a car you were purchasing, or indeed took the back off a TV, or the lid off the ... xed the cost will be immeasurable further down the line.It is hard to imagine how our leaders could think they have the neces-sary wisdom to sort out the world’s problems without the aid of...

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Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efﬁcient electron transfer and stable accumulation of photosystem I pot

Danh mục: Báo cáo khoa học

... transfer of excitation energyfrom the peripheral antenna to the core.In vitro the absence of PSI-J affects the stability of the PSI–LHCI complex. The results of the fractionation of mildly ... distinguish whetherit is the stability or the assembly of the PSI complex thatis affected needs further investigation. The reduced content of PSI was readily revealed by the appearance of the transgenic ... perturbation of the luminal side of PSI involving PSI-F and PSI-N. The absence of PSI-J might affect the conformation of PSI-F, which, in turn, changes the binding of PSI-N.PSI-F provides part of the...

Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot

Danh mục: Báo cáo khoa học

... with the A8B8isoform of GAPDH lead-ing to the A2B2isoform with a higher NADPH-dependent activity [39].By analogy with the CTE of the isoform of GAPDH[15,37], we hypothesize that the ... of the interaction site of CP12had not studied but our results give some clues withregard to the binding of CP12 to GAPDH. The impact of CP12 binding on the activity of GAPDH is also fur-ther ... Sparla et al.on the A2B2isoform of GAPDH [24,37], formation of adisulﬁde bridge in the oxidized CTE prevents the inter-action between the 2Â-phosphate of NADPH and the side chain of Arg77 (spinach...

Báo cáo khoa học: Classiﬁcation of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains pdf

Danh mục: Báo cáo khoa học

... due to the proximity of the negative c harge of the side chain of Glu706 from the neighboring subunit. The mutation of these residues might interfere with the oligo-merization required for the ... in the proteolytic site. The c rystal structure of the proteolytic domain of E. coliLon provided the final verification of the existence of the Ser–Lys dyad. Ala679, which replaced Ser679 in the inactive ... identi-fiable analogs of E. coli Lon and prompted a reanalysis of the active sites of this family of proteases. The alignment of the proteolytic domains derived from the sequences of > 100 Lon proteases...

Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot

Danh mục: Báo cáo khoa học

... ﬁrst in the presence of the inhibitor IpOHA [8]and later with product bound in the active site [9].There are several interesting features revealed by thesestructures. First, the active site contains ... organization of this enzyme is substantiallydifferent from that of the spinach enzyme with the active site constructed from two monomers of a dode-camer. In contrast, the active site of spinach ... or cofactor and in these cases the concentration of the nonvaried components wereincreased so that they would be at least 90% saturating.For the varied component, a preliminary estimate of the half-saturating...

Báo cáo Y học: Characterization of the active site of histidine ammonia-lyase from Pseudomonas putida pptx

Danh mục: Báo cáo khoa học

... of the imidazolyl moiety of L-histidine at the active site (Fig. 2A) and stabilization of the cationic intermediate arising from the imidazolyl moiety of the substrate (Fig. 2B). Because of the ... facilitates the electrophilic attack at the methyl-ene moiety of MIO by decreasing of the electron density in the p system of the CẳC double bond (Fig. 5).Calculation of the UV spectra of MIO and the ... incorporatingstates of the active site byMM1 calculations of the HYPERCHEM package [28]. All the mutated residues werefound within 12 A˚radii around the methylene carbon of MIO formed from Ser143. Therefore...

Báo cáo khoa học: Mapping of the active site of glutamate carboxypeptidase II by site-directed mutagenesis potx

Danh mục: Báo cáo khoa học

... available.Mutational analysis of the S1 site The model of the rhGCPII ⁄ NAAG complex suggeststhat GCPII most likely interacts with the N-terminalpart of the substrate via the side chains of Asp453,Asn519, ... discussedabove, the mutation Y552I leads to the decrease of both the Kmand kcatvalues. The mutation of Gly518to Pro led to a slight increase of the Kmvalue and oneorder of magnitude decrease of the ... substitutions farther away (Lys699, Asn257),most likely due to distortion of the coordinationsphere of the active -site zincs.Our results indicate that the binding of the glutaratepart of the inhibitor...

Báo cáo khóa học: Fidelity of targeting to chloroplasts is not affected by removal of the phosphorylation site from the transit peptide doc

Danh mục: Báo cáo khoa học

... removal of the phosphorylation motiffrom the transit peptides of tobacco and pea SSU did notFig. 4. The eﬀect of mutagenesis of the phosphorylation site in the transit peptide of tobacco SSU on the ... subcellular location of translation, and the receptorsat the outer organellar translocon. Lipids that are present at the chloroplast outer envelope and not on the outermembrane of the mitochondria, ... transitpeptides. Alteration of the phosphorylation site did notimpede targeting of the passenger GFP to the chloroplasts,nor was there any mistargeting to mitochondria. The pattern of GFP ﬂuorescence...

Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf

Danh mục: Báo cáo khoa học

... between the projection of the tilt of the z axis on the xÂ,yÂ plane (dened direction of tilt of z and the xÂ axis), and j % a + c denes the location of the rhombic (x, y) axes. The orientation of the ... at position E7 as the source of the H-bond to ligand on the basis of apartial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree globin ... in the 1H NMR data of Pe HbO2. The static structure of neither WT nor rWT metMb would allow the TyrE7 ringreorientation without signiﬁcant distortion of the hemepocket.Comparison to other...

Báo cáo khoa học: The catalytic role of the distal site asparagine-histidine couple in catalase-peroxidases potx

Danh mục: Báo cáo khoa học

... to assume that the disruption of the H-bondmakes the distal His less basic. As a consequence the pKavalue of the NeH of the distal His is lowered and the rateconstant for the reaction with ... toabout 10% of the value of native HRP. All these ﬁndingssuggest a similar role of the distal Asn-His couple in plant-type peroxidases. The present work also clearly showed that the basicity of the ... nm. The small shoulder at 368 nm indicates the presence of some free heme corresponding with the slightlyFig. 2. UV-Vis spectra of the ferric forms of Synechocystis wild-typeKatG and of the...