molecular biology of the glycine receptor
Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae pot
... determined the cDNA sequences of the EcRs and the USPs (RXRs) of Chilo suppressalis [14] and Leptinotarsa decemlineata [15] Dissociation constants of the binding of PonA to these receptors have ... (183–410) regions The C-regions of EcRs and USPs are highly conserved However, other regions, particularly the N-terminal parts of USP ⁄ RXR, vary The alignments of amino acid sequences of the A ⁄ B ... In the alignment figure (C) the amino acid residues that correspond to those important for the binding of PonA to the EcR of H virescens are boxed The arrow head indicates the 396th amino acid of...
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molecular biology of the parathyroid - tally naveh-many
... MOLECULAR BIOLOGY INTELLIGENCE UNIT NAVEH-MANY MBIU Tally Naveh-Many Molecular Biology of the Parathyroid Molecular Biology of the Parathyroid MOLECULAR BIOLOGY INTELLIGENCE UNIT Molecular Biology ... points in the PTH(1-34):PTH1 receptor complex, Ser1 of hPTH(1-34) to Met425 of the receptor4 3 and Lys13 of hPTH(1-34) to Arg186 of the receptor. 44 Our model of hPTH(1-34) bound to the PTH1 receptor ... from the human PTH cDNA sequence15 and later confirmed by the determination of the structure of the human gene.16 The amino acid sequence of the rat pre-peptide was derived from the sequence of the...
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Molecular biology of the lung and kidney of the african lungfish, protopterus annectens, during three phases of aestivation cystic fibrosis transmembrane conductance regulator, gulonolactone oxidase and p53
... organs in the lungfishes necessary for survival However, to date, there is a dearth of knowledge on the molecular biology of the lung of any lungfish species Therefore, the first objective of this ... studied the differential gene expression in the liver of P annectens at the end of the induction phase and the beginning of the maintenance phase Their results confirmed the importance of reconstruction ... level of airway surface liquid, a thin (10-30 µm) layer of fluid covering the luminal surface of the airway epithelium, is critical for the protection of the airway epithelium For transepithelial...
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EVOLUTION OF THE MOLECULAR BIOLOGY OF BRAIN TUMORS AND THE THERAPEUTIC IMPLICATIONS pdf
... Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications high grade gliomas The structure and compounds of the ECM of the brain tissue have many specific differences from other ... Hofmann M Characterisation of the murine gene encoding the intracellular hyaluronan receptor IHABP (RHAMM) Gene 1999;226:41 23 24 Evolution of the Molecular Biology of Brain Tumors and the Therapeutic ... Overexpression of dystroglycan decreased Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications the growth rate of glioma cell lines so it was found to be involved in the progression...
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Molecular analysis of the breeding biology of the asian arowana (scleropages formosus)
... each other in parental care The grey bar indicates the k value of the thief with the genetic father where the white 18 | P a g e bar refers to the k value of the thief with the genetic mother The ... attainable size, big and long fins of the RG1 and the intense colouration of the MG One of the shortfalls of the hybrid is the large variation of phenotypes produced in their offsprings which is common ... Figure 12: The breeding cycle of the Asian arowana in the farm The graph shows the total production in terms of number of batches（dotted line）and number of offspring (solid line) for the three...
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Molecular Biology of Secondary Metabolism - Case Study for Glycyrrhiza Plants
... export of the excess of reducing power via the malate valve, and productive consumption of the surplus of NADPH by the Calvin cycle Finally, as is the case with iron deficiency, the extent of protection ... transgenic N tabacum plants expressing the coding region of the chpA gene of Thermosynechococcus elongatus BP-1 in the cytosol under the control of the CaMV 35S promoter The transgenic tobacco plants ... somewhere in the evolution of vascular plants The case of Fld and Cyt c6 shows that reintroduction of these genes in the proper subcellular compartment of model and crop plants restored some of the selective...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL ... in the same direction with the heparin-binding domain at the N-terminal end [14] The other is a cluster of tryptophans (Trp390, Trp393, and Trp394) exposed to the surface (Fig 1A) The cluster of ... peak of wild-type LPL The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640 The fraction number of the high-affinity peak was the same for the wild type and the WW...
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Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx
... dimer are mainly limited to the conformation of the Glu-Pro dipeptide in the hinge region between the last b-strand and the core of CKS The crystal structure of the complex of CKS with CDK2 ⁄ cyclin ... whereas in the study on cyclin E degradation, Pin1 was brought in by the phospho-cyclin E and not by the CDC4a component of the final complex (Fig 2) [35] Therefore, the outcome of the molecular ... distinct molecular partners [6], and the conformation of a proline in the linker region between two SH3 domains of the Crp adaptor protein determines the autoinhibition of the domains [7] These...
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Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf
... on the sample (data not shown) We also analyzed the immunohistochemical localization of the enzyme in the kidney of Wistar Kyoto rats with immunoreactivity detected in the distal tubules of the ... was then analyzed To examine whether the enzyme is localized in the membrane, we Fig Detection of the immunoreactivity of P-LAP in the human renal cortex After incubation with 20 lgÆmL)1 of affinity-purified ... elucidate the role of vasopressin receptors, we examined the effects of vasopressin agonists and antagonists on the hormone-induced translocation of P-LAP in NRK52E cells (Table 1) The level of P-LAP...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... glycosaminoglycans [37] As the myc-his tag of the protein was present at the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was ... present on the surface of transfected cells, and had the expected molecular mass To test whether the presence of the myc-his tag at the C-terminus of CCR5 would interfere with the protein’s ability ... and receptors, respectively Purification of CCR5-myc-his For the purification of CCR5-myc-his we tested two different methods based on the presence of the six histidines at the C-terminus of the...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase II [29] The same phases are found in the corresponding ... this study, we present the molecular organization of the rst nonmammalian MGP gene and the functional analysis of its promoter We identied a region within the rst 70 bp of the xMGP promoter that ... representation of the xMGP promoter constructs used for transient transfections of A6 cells is shown to the left The nomenclature of the promoter deletions is based on the transcription start of the xMGP...
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Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc
... group at carbon of the guanine ring may interact with the side chain of Glu309, whereas the carbonyl group at position of the guanine ring may interact with the hydroxyl group of the Tyr239 side ... structures of the EhPGK obtained using the modeller program (Fig S1), it was found that the only difference in the amino acids that bind the purine ring was the presence of Val instead of Leu at ... to that of Gly312 in the pig enzyme On the other hand, the Leu residue present in the horse and yeast enzymes is conserved in the majority of PGK sequences reported to date; however, in the EhPGK,...
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Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf
... represent the geometric centers of the cavities that are located over the protein surface, and the blue spheres indicate the geometric centers of the cavities that are located inside the b-barrel The ... indicating the importance of the positive charges for the occurrence of nonspecific contacts with DNA [22] The BPV-1 protein shows low flexibility of the loop connecting the b2 and b3 strands (Fig 6B) The ... by filling the region between the two molecules with gap-spheres and then computing a three-dimensional density map that defines the surface of the gap region [26] In the interior of the HPV-16...
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Báo cáo khoa học: Alpha 1,3-fucosyltransferase-VII regulates the signaling molecules of the insulin receptor pathway potx
... However, all the above-mentioned structural changes in receptor glycans are located in the core portion of N-glycan, and whether alteration of the terminal residue on the outer chain of either N- ... expression of InR (including the a- and b-subunits) and EGFR both on the cell surface and in cells (Fig 2A,C), as well as the SLex content of the glycans of EGFR were unchanged, but the SLex content of ... be of interest to study whether the SLex level of InR on insulin-responsive cells in diabetic patients was changed This may reveal the role of the sugar chains on InR in the pathogenesis of diabetes...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... mouse brain This supports the hypothesis that the rate of transcription of CB1 in the striatum of symptomatic R6/2 mice is similar to the rate of transcription in regions of the brain where CB1 is ... affected the onset and rate of decline of CB1 mRNA levels Moreover, because the final steady-state level of CB1 mRNA was the same in both models of transgenic HD mice and the rate of decline of the ... the length of the CAG repeat or relative expression levels of the huntingtin transgene affected the rate at which the function of this factor was lost in the two lines of transgenic HD mice The...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... the AR coactivators ARA54 and ARA70, suggesting a specific role of these motifs in AR function [44–47] The increasing number of proteins found to interact with the AR LBD raises the question of ... fragment from pAR34 [23] into the corresponding sites of pGEX-2TK-CHB yielded pGSTAR.LBD Insertion of the AR LBD BamHI/SalI fragment of pGST-AR.LBD into the corresponding sites of pCMVGST [25] yielded ... triplicate, the mean of the highest AR.NTDwt value was set at 100% Each bar represents the mean (± SEM) luciferase activity of three independent experiments Fold induction is shown to the right of each...
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Reproductive Biology of the Laboratory Mouse docx
... Over 80 Years of Research Expertise Over 80 Years of Research Expertise Leveraging the power of genetics in the search for cures to human disease search for cures to human disease Nonprofit research institute founded in ... Resorption of fetuses of fetuses – Cannibalism of litters • Health status Health status • Seasonal effects Seasonal variation in inbred mice Seasonal variation in inbred mice Month of the year ... Cook MJ. 1965. The Anatomy of the Laboratory Mouse http://www.informatics.jax.org/cookbook/ Sperm Production Sperm Production • Diploid spermatogonial stem cells – lifetime supply • 12 stages of spermatogenesis...
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Reproductive biology of the marbled goby, Pomatoschistus marmoratus (Pisces, Gobiidae), in a northern Aegean estuarine system (Greece) pptx
... The rectangular part of the plot extends from the lower to the upper quartile; the centre lines within each box show the location of the sample medians; the crosses within each box indicate the ... 1990) The present work aims to study the reproductive biology of the marbled goby in the Aegean; more specifically to study the sex ratio, time of spawning, fecundity, and size at maturity of the ... goodness -of- fit test was undertaken to compare the sex ratios at each monthly sampling or within the size groups with the hypothesized sex ratio of 1:1 The gonadosomatic index (GSI), which describes the...
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Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
... is the detection of the modification, which involves the detection of a molecular mass change of Da in a relatively large protein substrate (20–45 kDa) Another challenge is the development of ... to that of PMT-C3 is that, at the sequence level, there is no discernible similarity of PMT-C3 to the proteins, with the exception of the Cryseobacterium protein glutaminase However, there is ... intoxication of PMT Little is known about the cellular uptake mechanisms of PMT Bacterial protein toxins are known to utilize a number of different entry routes, and the nature of the receptor often...
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Báo cáo khoa học: The molecular identity of the mitochondrial Ca2+ sequestration system potx
... earlier, the molecular identity of the protein(s) that actually form the PTP channel remains the most intriguing question On the basis of structural and other information, we hypothesize that the ... increased the conductance of artificial lipid bilayers in the presence of Ca2+, and the conductance was sensitive to ruthenium red [22], implying that it may be the Ca2+ uniporter or part thereof Further ... is driven by the pH gradient, in the case of the Ca2+ ⁄ 2H+ exchanger Elevated intramitochondrial Ca2+ can stimulate the activities of enzymes of the tricarboxylic acid cycle (TCA), thereby boosting...
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- physiology and molecular biology of salinity stress tolerance in plants
- calculate the molar mass and molecular formula of the solid
- tools of molecular biology harnessing the power of dna
- evolution of the molecular biology of brain tumors and the therapeutic implications
- histochemical methods neurochemistry and functional neurohistology including the molecular biology of neuron
- the roles of receptor associated protein rap as a molecular chaperone for members of the ldl receptor family
- biology of the postsynaptic glycine receptor
- identification of the glycine receptor
- cell biology of the glycine receptor
- differences in the molecular biology of adenocarcinoma of the esophagus gastric cardia and upper gastric third
- physiology and molecular biology of the developing circulation
- the molecular biology of learning and memory memory phases and signaling cascades
- cellular biology of the androgen receptor in the skeleton
- genetics and molecular biology of the major crop pest genus helicoverpa
- the molecular biology of sensory map plasticity in adult mammals
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