... histidine-rich Ca2+-binding protein, calsequestrin(CSQ) and CSQ-like proteins [3].Domain binding experiments [4], differential coimmunoprecipitation studies [5] and chemical crosslinking analysis[6] ... reduction in fast calsequestrin and triadin and a concomitant reduction in calsequestrin binding to these sarcoplasmic reticulum ele-ments. The calsequestrin-binding protein junctin was notaffected by ... overlaymethod in determining protein linkage. Its greatest advant-age is the direct visualization of proteinprotein interactionsunder controlled conditions. In contrast, other established protein biochemical...
... the initiation and development of ossifica-tion of spinal ligaments (OSL) [17]. Activities of BMPs areinhibited extracellularly by BMP-binding proteins such asNoggin and Chordin as well as intracellularly ... calcific tendinopathy and central one-third traumatic injury modelFigure 1Immunohistochemical staining of BMP-2 in colla-genase-induced calcific tendinopathy and central one-third traumatic injury ... histology and immunohistochemistryThe patellar tendon was washed in PBS, fixed in bufferedformalin and 100% ethanol, embedded in paraffin, cutlongitudinally to 5-μm thick sections and mounted...
... 2004;25:115–20.Sheth, Safety of Intranasal Steroids in Treatment of AllergicRhinitis 129 ORIGINAL ARTICLEEvaluating the Safety of Intranasal Steroids in theTreatment of Allergic Rhinitis Ketan Sheth, ... Parameters in Allergy, Asthma, and Immunology defined rhinitis as‘‘inflammation of the membrane lining the nose, character-ized by nasal congestion, rhinorrhea, sneezing, itching of thenose and/ or ... those with allergic rhinitis. Key words: allergic rhinitis, intranasal corticosteroids, safety, treatmentAllergic rhinitis (AR) affects almost 94 millionEuropeans, 50 million Americans, and 10...
... baseline high- sensitivity C-reactive protein level to cardiovascular outcomes withrosuvastatin in the Justification for Use of statins in Prevention; anIntervention Trial Evaluating Rosuvastatin ... subsequent adverseclinical events. The injury markers currently utilized arethe cardiac troponins, the myocardial band (MB) ofcreatinine kinase, and myoglobin. The findings of nor-mal confirmatory ... Samuel Turnipseed1 and Ezra A Amsterdam2AbstractObjective: High- sensitivity C-reactive protein (hs-CRP) rises with cardiac injury/ischemia. We evaluated its efficacy in aiding in the identification...
... Giraudier S, Vainchenker W, Wendling F. Prominent role of TGF-beta 1 in thrombopoi-etin-induced myelofibrosis in mice. Blood 2002;100:3495-503 32. Erusalimsky JD, Franklin R and Hong Y. Is ... on serum levels of TPO and the sIL-6 receptor. The latter was chosen since some recent evi-dence has been presented indicating that highlevels of this receptor might be found in MPD and might ... in platelet counts, TPO and sIL-6R concentrations in all MPD patients (All), in those achieving complete response (CR) and those attaining partial response or failure of response (PR+F), in...
... soluble protein before forming an insoluble protein precipitateby colliding with other recently translated proteins.Even though the majority of all proteins had a largefraction of soluble protein ... of the protein on theamount of soluble produced protein Because one aim of the present study was to assessinformation about protein solubility during protein production, the PrEST proteins used ... this correlation is highly depen-dent on the strain used for protein production (Fig. 3). In addition, there might be soluble proteins with inac-tive eGFP resulting in misleading results. Moreover,...
... (55%) of the fires were detected in cropland, 24% in wooded grassland, 8% in woodland, 7% in grassland, and 5% in forests. Because of the low fuel loading in cropland,emissions there were only ... at between 40 and 50 ppb in winter and spring(see inset in Fig. 17) and 30 ppb in summer. In fact, in thepast O3 levels have exceeded 70 ppb only on three other oc-casions during the periods ... peaked in March in the year 2002 andin April in the years 2001 and 2003,whereas in all three years there was very little burning in May. In 2002, a smoke episode caused by agricultural firesin...
... gly-cogen-targeting proteins GL and PTG but markedly reduced expression ofR6. Expression of R6 protein was increased in hepatocytes from Wistarrats after incubation with leptin and insulin. Diminished ... of hepatocytes with leptin and insulin (Fig. 8A) in agreement with previous findings [38]. R6 protein was increased by 75% after combined culture withleptin and insulin (Fig. 8B).DiscussionThe ... GLisone of four glycogen-targeting proteins expressed in liver [21–25]. These proteins have binding sites for pro-tein phosphatase-1 (PP1) and for glycogen, and theydiffer in their relative activities...
... large protein (p39/gp42) and M protein (gp33/gp36) [41]. Monoclonal anti-S used as a positive control could detect the S protein (p24/gp27) in addition to the L and M proteins.L protein M protein S ... protein M protein S protein anti-HBsanti-preS45.0kDa35.0kDa25.0kDaL protein M protein S protein anti-HBsanti-preS45.0kDa35.0kDa25.0kDaComparison of the binding ability of preS and preS1 ... Luo1,3 and Xiaofeng Zheng*1,2Address: 1National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, China, 2Department of Biochemistry and...
... levels of neurokinin A and anti-ribosomal P protein antibodies was also studied. Methods: Serum neurokinin A and anti-ribosomal P protein antibodies were measured in 70 autistic children in ... tried to find a possible link between the elevated serum levels of neurokinin A and anti-ribosomal P protein antibodies in autism. In this work, patients with elevated serum neurokinin A levels ... allergens, infectious agents and heavy metals), with a subequent increase in the release of this tachykinin from these cells. Neuroendocrine hormones, including tackykinins, triggered during stress...
... the possibility of protein chips providingmassively parallel, label-free analysis of protein- proteininteractions, and may eventually permit the analysis ofmultiple protein interactions simultaneously ... restriction sites in theopen reading frame encoding the STM protein scaffold[25,27]. The binding of CDK2 and CDK4 to the peptideaptamers was confirmed in vivo using yeast two-hybridinteraction ... functionalized with thedesired protein by incubating the device in 35 àl protein solution overnight in a sealed humid environment(Figure 4c). The adsorption of the protein, and the effective-ness...