determination of cell speci fi c receptor binding using a combination of immunohistochemistry and in vitro autoradiography relevance to therapeutic receptor targeting in cancer
Survey of cellular factors modulating the HIV 1 integration complex activity using a unique protein screening system in vitro
... additional PCR was performed using a common set of forward (5’GGGGACAAGTTTGTACAAAAAAGCAGGCTgcgaattcatcgatagatctgat-3) and reverse (5’GGGGACCACTTTGTACAAGAAAGCTGGGTCctacttgtcatcgtcatccttg-3’) primers to ... HIV-1 DNA specific primer (forward [M667]: 5’-GGCTAACTAGGGAACCCACTG-3’ and reverse [AA55]: 5’-CTGCTAGAGATTTTCCACACTGAC-3’ [Suzuki et al., Virus Genes, 2003]), 0.2 μM HIV-1 DNA specific fluorescent ... HIV-1 IN IN contains 288 amino acid residues and has three protein domains The NTD facilitates protein dimerization, CCD is involved in the catalysis of integration, and CTD has DNA -binding activity...
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Báo cáo lâm nghiệp: "Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations" pptx
... within the same colour of bars indicate non significant difference according to SNK test at P < 0.05 Figure Micropropagation, in vitro rooting and acclimatization of teak: (a) multiple shoots in ... Muganu M., Astolfi S., Caricato G., The effects of polyamines and hydrogen peroxide on root formation in olive and the role of polyamines as an early marker for rooting ability, in: Altman and Waisel ... peroxidase activity reducing IAA in the induction phase [23] Putrescine not only induces an increase in rooting percentage itself, but it requires a low concentration of auxin for improving root quality,...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... Proteins that speci cally recognize cisplatin-damaged DNA: a clue to anticancer activity of cisplatin FASEB J 12, 791–799 30 Kasparkova J & Brabec V (1995) Recognition of DNA interstrand cross-links ... presence of an excess of nonspeci c competitor calf thymus DNA mimicking randomsequence natural genetic material that can accommodate the cisplatin adducts regardless of the reactivity 4700 Fig Competition ... the facts that modification of particular sites in the genome of living cells is naturally stochastic on the one hand, and in uenced by the actual structural and functional state of the given chromatin...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... liposome -binding assay and surface plasmon resonance (SPR) analysis indicate that IC speci cally binds to membranes containing anionic phospholipids, rather than phosphatidylethanolamine (PE) A cellular ... membranes To gain insights into the association of IC with cellular membranes, we subsequently examined the intracellular localization of the inhibitor by fluorescence microscopy of living yeast cells ... fluorescence microscopic analyses using IC–GFP revealed the cellular localization of IC–GFP at the vacuolar membranes and lumens during the stationary growth phase, suggesting that IC is speci cally...
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Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
... Termination of transcription in human mitochondria: identification and purification of a DNA binding protein factor that promotes termination Cell 58, 391–397 17 Feranandez-Silva, P., Martinez–Azorin, ... Musicco, C. , Gadaleta, M.N., Quagliariello, E & Cantatore, P (1999) Cloning and characterisation of mtDBP, a DNA -binding protein which binds two distinct regions of sea urchin mitochondrial DNA ... sequences upstream and downstream of the canonical polyadenylation signal are important for protein binding to D-TERM DNA The binding speci city of D-TERM DNA and also the possible protein–DNA contact...
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Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf
... to BChl a; light blue: oxygen atoms coordinating to Ca2+ BChl a macrocycle induced by Ca2+ binding, all of which may account for the low absorptive transition energy of BChl a molecules in native ... as mCaCm (c, d) as mCaCm (a, b) mCbCb, s mCaCm (c) , mCN(III) s mCaCm (a) , mCN(II) CH3 bend, s mCaCm(d), mCN(IV) CH3 bend, C6 C16 mCN(I), dCmH (a, d), CH3 bend dCmH(d), CH3 bend mCN(III), dCmH(b), CH3 ... structural domain constituting the Ca2+ binding motif is found in a large number of Ca2+ -binding proteins, and is also known as the EF hand [16] Proteins containing the EF hand are divided into...
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Báo cáo khoa học: Unique proteasome subunit Xrpn10c is a specific receptor for the antiapoptotic ubiquitin-like protein Scythe docx
... dispensable for Scythe interaction DUIM1 indicates speci c elimination of amino acids 196–241, and DUIM2 indicates speci c elimination of amino acids 263–307 UIM1-N5 and UIM2-N5 indicate site-directed ... the Xrpn1 0c -speci c region was necessary and sufficient for Scythe binding (Fig 4B ,C, D) The most critical region for Scythe binding in Xrpn1 0c was the C- terminal region containing amino acids 331–339 ... Interaction of Xrpn1 0c with Scythe Y Kikukawa et al affinity for binding of polyubiquitin chains, indicating that UIM1 and UIM2 act in concert for polyubiquitin recognition in vitro [19] In addition...
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Báo cáo hóa học: " Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies" docx
... biology of infectious, replicating HCV virions Although one could debate as to which cell types are critical for inducing maladies in patients, that point will remain debatable and vary with the point ... that region are ACCCCCCCUCCCG, where the A was in position 119 The additions and deletions we are reporting here occur in the C' s proximal to A As shown in Figure 8A, the variation in our isolates ... changes, therefore lacked sequence commonality T-cells contained a mixture of Tcell subtypes, including CD4+ and CD8+ cells Guglietta et al [31] have suggested that CD8+ T-cells help to reduce...
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Báo cáo hóa học: "Research Article Motif Discovery in Tissue-Specific Regulatory Sequences Using Directed Information" doc
... nontissuespeci c In this work, genes which are either heart -speci c or brain -speci c are considered From the tissue -speci c genes obtained from the above approach, 45 brain -speci c gene promoters and ... sequences of a fixed length CONTRIBUTIONS Using microarray gene expression data, [9, 10] proposes an approach to assign genes into tissue -speci c and nonspeci c categories using an entropy criterion Variation ... Expression Atlas Training data Tissue -speci c sequences Neutral sequences Parse sequences to obtain relative counts Preprocess Build co-occurrence matrices for training data Feature (motif) selection...
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Báo cáo y học: "Immediate determination of ACPA and rheumatoid factor - a novel point of care test for detection of anti-MCV antibodies and rheumatoid factor using a lateral-flow immunoassay" pdf
... immunochromatographic device Lateral-flow immunochromatographic assay (LFIA) was manufactured as double antigen direct sandwich assay Devices (DCN, Carlsbad, CA, USA) for testing of up to 10 μl of biological ... Lajas C, Bellajdell B, Morado IC, Macarron P, Pato E, Fernandez-Gutierrez B, Banares A, Jover JA: Lag time between onset of symptoms and access to rheumatology care and DMARD therapy in a cohort ... rapid anti-CCP2 test compared to the anti-CCP2 ELISA Scand J Rheumatol 2008, 37:151-154 doi: 10.1186/ar3057 Cite this article as: Renger et al., Immediate determination of ACPA and rheumatoid factor...
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Thermal error modelling of machine tools based on ANFIS with fuzzy c means clustering using a thermal imaging camera
... structure This camera provides a sensitivity of 0.08 C, and an absolute accuracy of ± 2% Full camera specifications are provided in Table The thermal imaging camera offers a continuous picture of ... target variable The same test (120 heating and 70 cooling) was used for training and validation the models: experimental data are divided into training and checking datasets The training dataset ... of the machine surfaces Additionally, radiation reflects from shiny surfaces (ball screw, test mandrel, etc.), and is directly captured by the thermal camera and appearing as very hot areas In...
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A Guide to the Analysis of Fish Marketing Systems Using a Combination of Sub-sector Analysis and the Sustainable Livelihoods Approach potx
... boats and nets), but also the public infrastructure such as landing sites, market facilities and transport infrastructure Financial assets include cash, savings and access to formal and informal ... people can access and make use of their assets Natural capital Natural capital is the quality and quantity of natural resources that are available to people and above all, the access and control ... Page 42 Appendix Box A5 : Example of how changes in assets can be analysed and presented Natural capital Social capital Physical capital Financial capital Human capital Vulnerability prices may...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... Alternatively, a larger number of activator -binding domains could contribute to recruitment by increasing the probability of contact between activator and coactivator, leading to a faster on-rate ... transcriptional activators in vitro [33] Recombinant activator interaction domains interact with activation domains in vitro using a two-step mechanism, where rapid ionic interaction with an ... proteins are able to form speci c interactions with the large range of structurally distinct factors that they recruit to target genes The Swi1 and Snf5 activator -binding domains appear to work additively...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... Calculate the measured concentration of each analyte in each replicate, the mean concentration of each analyte in all replicates, and mean accuracy (as mean percentage of true value) for each analyte, ... calibration curve), calculation of response factor statistics (mean and standard deviation), and calculation of concentrations of analytes using either the calibration curve or the equation in ... sample analysis as dictated by results of continuing calibration checks After initial calibration is successful, a continuing calibration check is required each day or at the beginning of each period...
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Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx
... 2,5% and ambiguity rate ca 15% (J~rvinen, 1994) Of course the Estonian and English are too different languages and the comparison of performance of parsers not help to draw any fundamental conclusions ... Helsinki Tiina Puolakainen 1998 Developing Constraint Grammar for Morphological Disambiguation of Estonian Proceedings of DIALOGUE'98 Vol 2, 626-630, Kazan Atro Voutilainen, Juha Heikkil~i and Arto ... Proceedings of EACL '99 the attributes It can be explained by the fact that there are 12 types of attributes in ESTCG and the attribute tags are also added to almost every word in sentence (except...
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determination of methanol and ethanol
... Clemente St It has two traf c lanes in a single direction and near the sampling site has many residential and commercial buildings, as well as schools and clinical of ces The Botafogo beach—inside ... GC analysis was completed in about 12 Typical chromatograms, for standard and sample, are shown in Fig 2.3 Procedure Analytical curves for methanol and ethanol were done using external standards, ... of methanol and ethanol, are frequently in the range of several liters Classical sample collection into glass impingers containing water is difficult and troublesome for handling during field campaigns,...
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báo cáo hóa học:" Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson''''s disease" doc
... PCR assay The primers used for PCR assays were as follows: (1) DDC, forward: 5'-TTACTCATCCGATCAGGCACAC-3', reverse: 5'-GGCAGAACAGTCAAAATTCACC-3'; (2) DAT, forward: 5'-CGAGGCGTCTGTTTGGAT-3', reverse: ... analysis of the synthesis and release of DA by RPE cells HPLC analysis of the synthesis and release of DA by RPE cells. (A) HPLC analysis of standard of DA, 3,4-dihydroxyphenylacetic acid (DOPAC) and ... 5'CAGGGAGTTGATGGAGGTG-3'; (3) GAPDH, forward: 5'-CCATGTTCGTCATGGGTGTGAACCA-3', reverse: 5'GCCAGTAGAGGCAGGGATGATGTTC-3' PCR conditions were 95 C for 10 minutes, followed by 35 cycles of 94 C for...
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Báo cáo hóa học: " Fabrication of functional micro- and nanoneedle electrodes using a carbon nanotube template and electrodeposition" ppt
... pH sensor using a PPy-coated nanoneedle pH is one of the most important factors in chemical, biological, and medical applications In particular, intracellular pH is an interest factors to most ... requiring specific functionalization to bind particles to the needle body Page of Conducting polymers have some attractive electrical, chemical, and mechanical properties, which lead to unique advantages ... advantages for various applications, such as electronic devices, supercapacitors, actuators, and sensors In particular, conducting polymers have great potential as efficient chemical sensors and biosensors...
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reliability in automotive and mechanical engineering determination of component and system reliab pdf
... number of failures at a specific point in time or in a specific interval is not of interest but rather, how many components in total have failed up to a time or until a certain interval is reached ... mean can be significantly affected Variance The empirical variance s2 describes the average quadratic deviation from the mathematical mean and is thus a measurement for the statistical spread of ... practical trials, pilot-run series, production and quality controlling correct operation and maintenance, correct use and application Figure 2.20 The “bathtub curve” calculations, practical trials...
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Báo cáo khoa học: " Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos" pps
... dezylana erew ataD sisylana lacitsitatS lortnoc evitisop a sa desu saw FFp-MCT dessessa saw )4 tnemirepxE( TNCS dna )3 tnemirepxE( AP retfa yticapac latnempoleved tneuqesbus dna noitarutam etycoo ... yrotarobal eht ot detropsnart dna riottaba lacol a ta detcelloc erew stlig latrebup-erp morf seiravo enicroP MVI dna noitcelloc etycoO ]92[ etatcal Mm 0.5 dna etavuryp Mm 5.0 htiw esoculg gnicalper ... gniniats 24333 tshceoH gnisu dessessa saw stsycotsalb ni rebmun llec latoT )0 yaD detangised saw TNCS fo yad eht( ylevitcepser ,6 dna syaD no detaulave erew noitamrof tsycotsalb dna egavaelC syad...
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