cloning expression purification and immunological characterization of proteins encoded by regions of difference genes of mycobacterium tuberculosis
Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
... GluRS and GluTR, whose spectrum and DLS signal are shown in Fig S11 The migration positions of GluTR and GluRS, were determined by comparison with those of a homogeneous sample of GluRS (S) and of ... ubiquity and essentiality of aaRS makes them of interest as targets of new anti-infectives [3] Their reaction formally consists of the activation of the amino acid by adenylation (Eqn 1) followed by ... amidotransferase and the enzymes of the C5 pathway of ALA biosynthesis are essential for M tuberculosis For these reasons, with the dual goal of contributing to understanding of the metabolism of this...
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Báo cáo y học: " Transactivation and signaling functions of Tat are not correlated: biological and immunological characterization of HIV-1 subtype-C Tat protein" docx
... representation of isolation and purification of the Schematic representation of isolation and purification of the Tat protein One serious limitation to recombinant protein expression in E coli is the copurification ... or above), whereas that of most of the E coli host proteins is acidic, below pH [40] We took advantage of the difference in the pI values of Tat and E coli host proteins and employed an anion-exchange ... quantities of the protein but also the isolated protein was often completely or partially inactive Results and discussion Purification and characterization of the recombinant Tat proteins Although...
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Báo cáo y học: "Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana" ppsx
... extracts of soluble fractions lanes 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8) Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, ... sequences of the four genes Separate sets of primers were then designed for amplification of the genomics DNA sequences of the genes and for cloning into the E coli pET43a-based protein expression ... bassiana proteins expressed in E coli fractions of analysis of soluble and pellet (inclusion bodies) SDS-PAGE SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B bassiana proteins...
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Báo cáo khoa học: Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase ppt
... supported Characterization of Mycobacterium tuberculosis PncA by the National Institutes of Health (NIH) grants AI44063 and AI49485) The other authors were supported by TB Research Projects of the ... and were subsequently sequenced to confirm the presence of the site-directed mutations Protein overexpression and purification The wild-type and mutants of PncA were overexpressed and purified by ... Selection of the conserved residues and site-directed mutagenesis Metal ion contents The PncA sequence of M tuberculosis H37Rv was compared with those of P horikoshii, Mycobacterium smegmatis and E...
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Báo cáo y học: "Glutathione and growth inhibition of Mycobacterium tuberculosis in healthy and HIV infected subjects" pot
... AIDS Research and Therapy 2006, 3:5 intracellular M tuberculosis by treatment with LPS (a stimulus for TNF-α expression, via triggering of toll-like receptors) and IFN-γ (a product of activated ... progression of both diseases In our studies, we observed that NAC treatment caused down-regulation of the synthesis of IL-1, IL-6, and TNF-α (Fig 4a, 4b, 4c), and up-regulation of the synthesis of IFNγ ... CD4(+) and CD8(+) T cells kill intracellular Mycobacterium tuberculosis by a perforin and Fas/Fas ligand-independent mechanism J Immunol 2001, 167(5):2734-42 Cohn DL, El-Sadr WM: Treatment of latent...
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Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases docx
... dose-dependence of the regulation of the activity of the zebrafish ST by these divalent metal cations and their modes of action Fig Effects of divalent metal cations on the sulfating activity of the zebrafish ... Detection of zebrafish SULT1 ST1 and ST2 mRNAs and (B) Western blot analysis of zebrafish SULT1 ST1 protein (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs in cultured zebrafish cells (lanes and 3) and ... cytosolic STs and their biochemical characterization We report in this communication the molecular cloning and expression of two distinct zebrafish cytosolic STs The enzymatic activities of purified...
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Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc
... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification ... 23th candidate proteins had scores from 82 to 178, those were all coat proteins of various isolates of LSV, and protein scores greater than 67 were significant (p < 0.05) 15 of 28 mass values of ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... the presence of two distinct bands between 1000 and 1100 bp of roughly the same extent (not shown) Cloning and nucleotide sequencing of these two products indicated that the lower band (1011 bp) ... amplification and cloning of ACMSD isoforms Human brain, liver and kidney total RNA (Clontech Laboratories, Inc., Mountain View, CA, USA) was reverse transcribed in the presence of random primers, by using ... activates ACMSD expression directly by site-specific binding to the promoter, and the latter represses ACMSD expression indirectly through suppression of HNF4a expression [30] The presence of ACMSD has...
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Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... of primary amino acid structures of EcR and USP from L decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement of the binding affinity of ... 6.29 and L migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dipteran and lepidopteran insects Thus, the functions of USP of L decemlineata, T molitor and L ... result of this study The degree of mobility shift of a band which is caused by monomeric binding of LdEcR-A alone should be much smaller than that by LdEcR-A ⁄ LdUSP However, the degree of band...
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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
... proteins J O Schulze et al study has revealed expression proles for most of the human transcripts and uncovered different tissuespecic expression of Iba1 and Iba2 [5] For Iba1, preferential expression ... composed mainly of a helices (Figs and 2) The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) anking a ... it may be concluded that EF-hand of the Iba proteins is not capable of functional Ca2+ binding Function of EF-hand In both Iba1 crystal structures, the loops of EF-hand adopt essentially the same...
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Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx
... in the case of Pf LDH and red in the case of Pf MDH Comparative expression of Pf MDH, Pf LDH and Pf MQO and effect of gossypol The effect of gossypol on the expression of Pf LDH and Pf MDH in ... after h of exposure at the late trophozoites stage Expression the enzymes was checked by (A) analysis of equal amounts of RNA by Northern blotting and also by (B) analysis of equal amounts of proteins ... semiquantitative analysis of transcripts by Northern blotting and also by quantification of the enzyme protein by Western blotting Expression of Pf MDH, Pf LDH and Pf MQO in control and drug treated P falciparum...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... M tuberculosis was identified In this report, we describe the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of ... Occurrence of trehalose and molecular cloning and characterization of trehalose-6-P synthase homologues J Exp Bot 52, 1817–1826 Zaragoza, O., Blazquez, M.A & Gancedo, C (1998) Disruption of the Candida ... glucose-6-P, was detected and quantified by coupling the conversion of phosphoenolpyruvate to pyruvate by pyruvate kinase, and the conversion of pyruvate to lactate by lactate dehydrogenase In...
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Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf
... coding regions of these ATs were subcloned into another expression plasmid, pET-41a(+), instead of pRSET-B DNA sequences of the coding regions of these expression plasmids were confirmed by sequencing, ... d-amino acids, was cloned and characterized This represents the first cDNA cloning and functional characterization of a d-AAT of eukaryotic 1196 origin Further characterization of this d-AAT is necessary ... This is the first report, for eukaryotes, of cDNA cloning and functional characterization of d-AAT acid aminotransferase in A thaliana tion rate of main roots and hypocotyls appeared to be greater...
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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt
... proliferative and differentiation state, basal level of ERK1 ⁄ and Akt kinase phosphorylation, and P2Y1 and P2Y12 receptor protein expression and functional activity Examination of the effects of specific ... characterized by increased expression of the P2Y12 receptor and low expression of the P2Y1 receptor In cells grown in medium containing 10% (v ⁄ v) fetal bovine serum, the expression ratio of the two ... SD and are representative of three separate experiments 1974 morphology were not caused by differentiation of the cells into the oligodendrocytic lineage In this study, the low expression of...
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Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc
... insensitivity of front-end desaturases and elongases to the presence of an x3-double bond and of x3-desaturases to the structure of the carboxyl end of the fatty acids, the existence of separate x3- and ... this domain and the desaturase part [32] Functional characterization in Saccharomyces cerevisiae The activities of the proteins encoded by these two cDNAs were confirmed by heterologous expression ... we report the cloning and functional characterization of a D5- and a D6-fatty acid desaturase from P tricornutum Both contain the typical features of membrane-bound desaturases and a cytochrome...
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Báo cáo sinh học: " Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes" ppt
... increased the levels of the L1 proteins encoded by the two PV Nat L1 genes (Fig 2B) The down- and up-regulated responses of the L1 protein expression from the PV Mod and Nat L1 genes to post-transfection ... treatment of methylcellulose also differentially regulated expression of the L1 proteins from PV Nat or Mod L1 genes, confirming further that expression of the L1 proteins from PV Nat and Mod L1 genes ... resulted the KCs to change expression patterns of the other KC differentiation markers by reducing expression of basal keratins K14 and increasing expression of keratins K1 and K10 (data not shown)...
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Báo cáo hóa học: " Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes" pptx
... increased the levels of the L1 proteins encoded by the two PV Nat L1 genes (Fig 2B) The down- and up-regulated responses of the L1 protein expression from the PV Mod and Nat L1 genes to post-transfection ... treatment of methylcellulose also differentially regulated expression of the L1 proteins from PV Nat or Mod L1 genes, confirming further that expression of the L1 proteins from PV Nat and Mod L1 genes ... resulted the KCs to change expression patterns of the other KC differentiation markers by reducing expression of basal keratins K14 and increasing expression of keratins K1 and K10 (data not shown)...
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báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx
... genes of AtERF13, we determined the changes in the expression levels of a number of ABA-responsive genes by Real-Time RT-PCR Page of 13 Among the genes we investigated, the expression level of ... #6 #3 Figure Expression of ABA-responsive genes in AtERF13, RAP2.4L and RAP2.4 overexpression lines Expression of three ABA-regulated genes (COR15A, ADH1 and RAB18) was determined by Real-Time ... lines of RAP2.4 and RAP2.4L Top left, expression levels of RAP2.4 and RAP2.4L in the single knockout lines of RAP2.4 (RK) and RAP2.4L (RLK) determined by RT-PCR Top right, expression levels of RAP2.4...
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Structural and binding characterization of the antiviral host proteins, VIPERIN and VAPC
... Construction 35-36 2.2.2 Protein Expression and Purification 2.2.2.1 Expression and purification of insoluble proteins 37 2.2.2.2 Expression, Purification and Reconstitution of the [Fe4–S4] cluster in ... Purification 3.2.5.1 Expression and purification of full length VAPC and V truncated constructs 3.2.5.2 Expression and purification of NS5B 73-74 74-75 3.2.6 Preparation of Isotope Labeled Proteins 76 ... structure prediction diagram of Viperin (Arrow represents the truncation site) 36 2.2.2 Protein Expression and Purification 2.2.2.1 Expression and purification of insoluble proteins The recombinant...
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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model
... Figure 4.1 Morphology and colony formation of cord lining cells……………………77 Figure 4.2 Protein expression of HLA-G and HLA-E in CLECs……………………79 Figure 4.3 mRNA expression of HLA-G and HLA-E in CLECs……………………81 ... treatment of a type diabetes mouse model xiv LIST OF FIGURES AND TABLES Figure 3.1 Schematic of human umbilical cord and the isolation of cord lining cells 51 Figure 3.2 Schematic of mixed leukocyte ... exogenous insulin Even with the use of insulin premixes and analogs and close glucose monitoring, the risks of postprandial hyperglycemia and hypoglycemia are present and long-term complications may...
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