autocrine loop for survival as an important prerequisite for axon regrowth after injury
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... MIP-1a and then the CCR5 present on the plasma membrane was quantified by FACS analysis using the 2D7 antibody (Fig 2B) It was found that the 60–70% of the myc-his tagged protein was internalized, as ... were transfected with pcDNA3 containing the ccr5-myc-his gene Expression was measured by FACS, using the 2D7 anti-CCR5 Ig [36] Western blot analysis was carried out using an anti-myc Ig Transfected ... MIP-1a has been described before [16] We therefore attempted to use another ligand, RANTES, to compete with MIP-1a for specific binding sites on proteoliposomes; partial competition by RANTES and...
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Báo cáo khoa học: Involvement of NF-jB subunit p65 and retinoic acid receptors, RARa and RXRa, in transcriptional regulation of the human GnRH II gene pot
... cells is defined as 1.0 Total RNAs were harvested 24 h after drug treatment Firststrand cDNAs were prepared from total RNAs as described and used for quantitative PCRs The hGnRH II transcript level ... Immunoprecipitation without antibody (No IP, lane 3) and using a nonspecific antibody against rabbit IgG (IgG, lane 7) was carried out as negative controls Lane 8, a negative control for PCR (without any DNA template) ... mechanism is important as a means of attenuating and counterbalancing hormone-induced transactivations, acting transiently as part of a cycle of cofactors at the target promoters, and allowing...
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Báo cáo khoa học: ABCG transporters and disease pdf
... until very recently As was the case for ABCG5 and ABCG8, an important physiological function of ABCG2 was uncovered through genetic studies of human disease In a series of genetic and physiological ... breast milk [58] In contrast, there is some evidence that human breast milk plays an important role in delivering antioxidants, including urate, to infants [59] Interestingly, while human breast ... individuals of European ancestry, including replication in an additional 11 000 European ancestry and 3800 African American study participants, identified common alleles in ABCG2 as associated with...
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báo cáo hóa học:" Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways" ppt
... intraepithelial neoplasia, hemangiomas, lymphomas, angiosarcomas, myelodysplastic angiogenic syndrome and other angiopathies [2123] The HIV-encoded transcriptional transactivator (Tat) protein has been implicated ... pathways, Rahim Hashim for modifying figures; Vivek Bhatt, Sher A Khan and Rahim Hashim for reading the manuscript; and summer students, Asad Arastu, Varun Devraj, Sarah Hussain, Ashwan Mehra for their ... enhanced cell growth and invasion necessary for angiogenesis and metastasis [121,122] Experiments in mouse embryo cells have shown that viral CRK is also essential for transducing signals for...
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báo cáo khoa học: " A strong constitutive ethylene-response phenotype conferred on Arabidopsis plants containing null mutations in the ethylene receptors ETR1 and ERS1" ppsx
... regions of ETR1, anti-ETR1(165–401) and anti-ETR1(401–738), as well as with the anti-(H+-ATPase) antibody as a loading control Wild-type ETR1 migrates at a molecular mass of 77 kDa The asterisk indicates ... accompanying Quantity One software For RT-PCR analysis of the etr1-9 mutant, total RNA was extracted from plants, treated with RNase-free DNAse (Invitrogen), and used to make first strand cDNA ... 2dose-response analysis for etr1 and ers1 single mutants Ethylene Ethylene dose-response analysis for etr1 and ers1 single mutants The etr1-7 mutant is of ecotype Columbia (COL) The etr1-9, ers1-2, and...
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Báo cáo khoa học: " Monocarboxylate Transporters and Lactate Metabolism in Equine Athletes: A Review" pdf
... diffusion, Na+/H+ exchange and MCT - shows that MCT plays the major role; for instance, in hu- Figure Transport of lactate and protons across membranes Lactate Transport and Metabolism man muscles the ... Samples for the measurement of whole blood lactate and plasma lactate were taken about after a 2100-m race The sample for plasma lactate analysis was immediately cooled in ice-water and centrifuged ... lactate transported from the muscle was the same However, when part of the blood samples mentioned was immediately put on ice-water and plasma was rapidly separated by centrifugation and the plasma...
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Báo cáo y học: " Expression of eicosanoid receptors subtypes and eosinophilic inflammation: implication on chronic rhinosinusitis" pdf
... instructions (Oxford BioMedicals, Oxford, USA) The detection ranges for all assays were between 0.02–10 ng/ml The sensitivity was of 0.2 ng/ml for all assays and the intra-and inter-assay coefficient ... rhinosinusitis and nasal polyposis and following our results, we question its role in these diseases As well as leukotrienes, prostaglandins and especially PGE2 play an important role in the ... congestion, lost of smell, changes in sense of taste and persistent postnasal drainage), clinical examination, nasal endoscopy and paranasal sinuses CT-Scan, defined as presence of visible bilateral...
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Báo cáo khoa học: "Steroid hormone receptors ERα and PR characterised by immunohistochemistry in the mare adrenal gland" ppt
... Persson for kind provision of the primary antibodies for ERα and PR, to Åsa Jansson for preparation of the tissues for immunohistochemistry and to Mari-Anne Carlsson and Åsa Karlsson for assistance ... samples for oestradiol and progesterone analyses were collected prior to euthanasia from all ten mares Samples were immediately centrifuged and the plasma stored at -18°C until assay For a summary ... OVX+Oe < 0.1 35.0 - 743 OVX+Oe None None Oestradiol* 20 h before euthanasia Oestradiol* 22 h before euthanasia Oestradiol* 18 h before euthanasia < 0.1 59.0 - 478 453 464 502 * Oestradiol benzoate...
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Báo cáo y học: "Membrane transporters and protein traffic networks differentially affecting metal tolerance: a genomic phenotyping study in yeas" potx
... cadmium/nickel resistance, we over-expressed both repressors and tested metal tolerance of the resulting transformants As shown in Figure 5b, an increase in cadmium/nickel tolerance was observed in ... 2.5 mmol/l NiCl2 for sensitive strains, and 50 μmol/l CdCl2 and 3.5 mmol/l NiCl2 for strains scored as resistant) and growth was examined after incubation at 30°C for to days Mutant strains exhibiting ... PVC to the late Golgi and to the plasma membrane [77,80,81] Mutational inactivation of these pathways can lead, for instance, to an abnormal accumulation of plasma membrane transporters that may...
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The role of adenosine, adenosine receptors and transporters in the modulation of cell death
... Depending on vascular tone and endothelial integrity, ATP acts as a vasodilator or vasoconstrictor (Kennedy & Burnstock 1985) Both hypoxanthine and xanthine are substrates for xanthine oxidase, the ... adenosine transportation was traditionally believed to play an important role in adenosine metabolism, further studies showed that it is an important determinant of the pharmacokinetics, plasma and tissue ... transmembrane domains are connected by three extracellular and three cytoplasmic hydrophilic loops of unequal size Typically the extracellular loop between TM4 and TM5 and the cytoplasmic loop...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... two-exponential association model kobs(rapid) and kobs(slow) are the observed association rate constants for the rapid and slow association phases, respectively The values for the constants are means ± standard ... PCSK9-D374Y for LDLR at neutral pH (see below) The extent of binding to blank wells was similar for both ligands, and the radioactivity associated with cells was at least 10 times of the counts associated ... remained bound after h was about 45% The dissociation rate constants [koff] for the rapid phase [koff(rapid)] and for the slow phase [koff(slow)] are shown in Table Taken together with the association...
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Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression profiling ppt
... sources: GenBank (http://www.ncbi.nlm.nih.gov/) for D melanogaster, Ap mellifera and Homo sapiens, VectorBase (http://www.vectorbase.org/) for Anopheles gambiae, and Beetlebase (http://www.bioinformatics.ksu.edu/Beetle ... amplification of AaTll and AaPNR-like from the mosquito fat body corresponds with a basal level of transcription, as previously discussed for AaHR51 and AaDsf AaSvp transcript abundance was high in the ... endoplasmic reticulum and Golgi complexes and ribosome proliferation [2,45] In D melanogaster, DmHR78 is required for growth and viability during larval stages, as demonstrated by DmHR78-null mutants...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... especially in higher animals (mammals and also in frogs and fishes), an aspartate (aspartic acid 248 in human 4F2hc; aspartic acid 380 in Fig as both the N-terminal and transmembrane segments are ... specificities, such as oligo-1,6glucosidase, dextran glucosidase, trehalose-6-phosphate hydrolase and isomaltulose synthase, whereas the representatives of trehalose synthase, amylosucrase and sucrose ... means that in basal Metazoa an ancestor of both the present-day 4F2hc and rBAT proteins was separated from the GH13 enzymes The ancestor acquired the N-terminal and transmembrane segments and,...
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Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx
... °C and at 72 °C In the first 30 cycles the annealing temperature was automatically decreased by 0.5 °C for each cycle After this, another 35 cycles of 95 °C for 45 s, 50 °C for 30 s and 72 °C for ... product The cells were grown for 48 h after transfection before harvesting For semistable transfection, HEK 293-EBNA cells were transfected as described above and grown for 24 h The cells carrying ... Bergqvist for skilful technical assistance; Ulf Hellman (The Ludwig Institute for Cancer Research, Uppsala, Sweden) and Marie Sundqvist (Uppsala University, Sweden) for peptide analyses; Dana Hutchinson,...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... two bands (at 39 and 80 kDa) for D2S, but only one band (85 kDa) for D2L receptors (Fig 3, lane 3) The smaller band (39 kDa) observed for D2S may correspond to a disruption of an oligomeric form ... plate and the fluorescence signal was monitored using an AnalystTM (Molecular Devices) or an Ultra-384 (Tecan) fluorimeter configured for time-resolved fluorescence The Eu3+-labelled anti-FLAG Ig was ... gp120 (clone 11/4C) and the FLAG sequence were used, and Fig shows the band pattern visualized by means of secondary conjugated antibodies Anti-gp120 Ig and anti-FLAG Ig identified bands corresponding...
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Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx
... manufacturer’s protocol and reagents Sequence analysis Searches for homologous proteins on GenBank databases (National Center for Biotechnology Information, USA) were performed using the BLAST ... possesses an aspartic acid at position 21 whilst other sequences have an asparagine, Wntx-5 has a lysine at position 29 whereas other sequences have a methionine, and Wntx-9 has an asparagine ... chain assembly was performed on a standard Applied Biosystems 431 peptide synthesiser cDNA of the chimeric a7-V201–5HT3 was kindly provided by J P Changeux (Pasteur Institute, Paris, France.) and...
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Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc
... 133 transport activity DmNST was located in the Golgi region, as was hUGT1 (panels e and f) In Western blot analysis, the DmNST was detected as a broad band with an apparent molecular mass ranging ... transformant, and analyzed them for the presence of the DmNST protein by Western blot analysis using anti-HA Ig (Fig 4) DmUGT-cHA migrated as a broad band with an apparent molecular mass ranging ... (lanes and 2) and Lec8 (lanes and 4) cells Cell extracts were prepared from cells transfected with appropriate plasmids as speci®ed below, and were subjected to Western blot analysis Lanes and...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... transformed with ligation products for amplification Expression was carried out in E coli BL21(DE3) as periplasmic protein All transformed clones were selected in 50 lgÆmL)1 kanamycin plates An ... Materials and methods Reagents All chemicals were of analytical grade and purchased from Sigma (St Louis, MO) Restriction enzymes, Taq DNA polymerase, T4 ligase and buffers for cloning were purchased ... a resonance mirror using an IAsys instrument biosensor SSA affinity for the immobilized TCR Vb5.2 was first measured and data were evaluated by Scatchard plot analysis Dissociation constants were...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... purchased from PAA Laboratories (Colbe, Germany) Fugene was from Roche ¨ (Mannheim, Germany) and Invitrogen supplied hygromycin B and blasticidin Poly(lysine), captopril, 1.10-phenanthroline and ... 37 °C for 30 It was stopped by exchanging the buffer with 0.75 mL of ice-cold 20 mm formic acid and by transferring the tray onto ice for additional 30 As a baseline control one tray was left ... and 2.5 lL of antiserum AS3 46 for h at °C The mixture was then washed twice with RIPA buffer and once with distilled water, resuspended in 30 lL of Laemmli buffer and incubated for at 95 °C After...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... through the formation of aggregates and degradation before they can be transported to the plasma membrane [13,14] The failure of ORs to translocate efficiently to the plasma membrane was also associated ... confocal microscopy of the I7 OR in yeast Optical (left panels) and confocal (right panels) visualization of spheroplasts from nontransformed MC18 yeast and yeast transformed with pJH2-I7 expression ... level ever achieved for any GPCR membrane expression in yeast Discussion In this study we developed a novel, robust and sensitive yeast-based bioassay for odorant screening Yeast was engineered to...
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