assessing idps in the living cell
Hiệu quả của việc bổ sung chế phẩm axit amin tổng hợp L- Lysine và DL-Methionine trong khẩu phần lợn thịt F1 (MóngCái –Yorkshire) nuôi ở trại Tiền Phong
... protein thấp phải bổ sung axit amin kết tinh (Brudboid Southern, 14 1994) Như vậy, vấn đề quan trọng dinh dưỡng lợn axit amin thiết yếu Các axit amin thiết yếu lợn là: Arginine, histidine, leucine, ... izoleucine, lysine, methionine, phenylalanine, threonine, tryptophan valine Riêng lợn giai đoạn trưởng thành cần loại axit amin trừ arginine Trong thực tế, vài axit amin loại thay cho như: Cystin ... methionine lợn Ví dụ: Nhu cầu Methionine 0,6% 0,3 % Methionine Cystin bù đắp Cystin methionine axit amin chứa lưu huỳnh Methionine tổng hợp từ Cystin phải cung cấp từ thức ăn Tyrosin phenylalanin...
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Tài liệu Báo cáo khoa học: The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d1 biosynthesis doc
... precorrin-2 in vivo and in vitro Results and Discussion Production of recombinant P aeruginosa NirE The recombinant P aeruginosa NirE protein was produced either as a fusion protein carrying a C-terminal ... homology with the two other SUMTs from P aeruginosa (CysG, CobA; Fig 1C) that are involved in siroheme and cobalamin biosynthesis Therefore, it was proposed that the NirE protein could be the SUMT ... protein carrying both N-terminal thioredoxin (Trx)- and S-tags and a C-terminal His-tag (Trx-S-NirE-His) In both cases the recombinant protein was purified to apparent homogeneity in a single...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... domain or full-length MSR results in a quenching of the intrinsic flavin fluorescence, suggesting that the flavin chromophore is shielded from the solvent in the protein–protein complex [19] From the ... of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark ... footnote) in the reaction mixture may reduce a proportion of the FMN domain, converting some of the enzyme to the active form The 3-fold increase in activity may arise from the binding of the FMN...
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Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx
... the subject of continuing investigation in this laboratory To elucidate further the mechanism of action of the substituted benzo-fused heterocycles, to explore the interactions occurring at the ... interactions between the pterin ring and MeTr based on the binding of hydroxymethylpterin pyrophosphate to DHPS Following the same approach, and in order to identify the orientation of the substrate ... remain the same Docking of the substrate 5-methyltetrahydrofolate in the model of the active site was based on the approach followed by Doukov et al [30] to identify the interactions between the...
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Tài liệu Báo cáo khoa học: Abstract Integration of Metabolism and Survival PP-1 The metabolic switch in liver methionine metabolism pptx
... mediates the suppression of the brain in ammation in the LPSinduced experimental brain in ammation model in vivo PP-27 Transglutaminase inhibition promotes sensitivity to the chemotherapy in cancer cells ... ‘below’ the Q119 guanidiniums of the four domains, holding the domains together and blocking the channel Adding charges (one proton per domain) breaks the plug, the side chains separate, and the ... VRK2B in vivo Furthermore, the two VRK2 isoforms interact by its N-terminal domain with JIP1 in vitro, binding the C-terminal region of JIP1 The kinase activity of VRK2 is not necessary for the interaction,...
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Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc
... the methionine and threonine biosynthesis pathways In the following the model is used to investigate the sensitivity of the two-enzyme system to the different input variables and to explain the ... one (indicated beneath the graphs abscissa) were set at their values in an illuminated leaf cell chloroplast (Table 2) The dotted lines in the graphs indicate the value of the changing input ... Pi inhibition and CGS ping-pong mechanism on the branch-point properties The analysis provides insights into the mechanisms of control of methionine and threonine syntheses in plants The mathematical...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... residuals using the crystalline coordinates of the free E coli PEP carboxykinase [2] The deviation of the resulting model structure (P-pyridoxyl labeled protein) from the coordinates of the starting ... for the magnesium cation in the protein The incubation of the labeled enzyme with increasing concentrations of adenine nucleotides in the presence of Mn2+ led to a progressive enhancement of the ... double binding function increased 1.52-fold Interestingly, CO2 binding was not affected by the presence of cations The saturation curves in the absence or presence of Mn2+ were similar, Kd in the...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... GAMT in sarcoma and in two other malignant cell lines compared to the levels of these two creatine synthesizing enzymes in normal muscle Thus, to determine whether this up-regulation is taking ... creatine biosynthesis, AGAT and GAMT, in all three types of malignant cell lines suggests the possible presence of creatine in these cells However, as noted above, the intrinsic level of creatine ... low in these cells Thus, we studied the uptake and release of creatine in EAC and S180 cells as a model system Figure shows that, if the cells were incubated for h in the uptake medium containing...
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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt
... causes protein modification We investigated whether the recombinant EhMGL was modified after the incubation with TFM by examining the mobility of the proteins by SDS ⁄ PAGE When recombinant EhMGL1 ... activity following preincubation with Met was indistinguishable from that without preincubation, confirming that the decrease was not due to inactivation of MGL during the preincubation These results ... for the amoebic MGL itself, we speculate that carbonothionic difluoride generates crosslinks surrounding proteins in the cytosol of the parasite, leading to the observed toxicity to the cell The...
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Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
... side chain slithers along the side of the protein surface, whereas in the M100K structure it points out into the solvent (Fig 4C) The position of the K99 side chain is also different In the wt ... the coordinating K100, with the H-bonded water molecule found in the X-ray structure increasing the electron donating power of the coordinating amino group which in turn can decrease the reduction ... changes being minimal the trapping of two main-chain conformations and increased B-factors at 295 K suggests that the presence of the coordinating Lys in some way can in uence the dynamics of the ligand...
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Báo cáo khoa học: "Boosting Statistical Word Alignment Using Labeled and Unlabeled Data" ppt
... interpolated model using the unchanged supervised model and the rebuilt unsupervised model Then the interpolated model is used to align the training data Since the training data includes both labeled ... the occurring frequency of the alignment link (e, f ) in the word alignment results of the training data produced by the model M l 4.2 Method This method only uses the labeled data as training ... rebuild the supervised model by estimating the parameters as described in section with the weighted training data Then we interpolate the supervised model and the unsupervised model to obtain an interpolated...
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Báo cáo khoa học: "Automatic construction of a hypernym-labeled noun hierarchy from text" docx
... use the same parsed text described in the previous section As suggested in Hearst (1992), we can find some hypernym data in the text by looking for conjunctions involving the word "other", as in ... evaluated the noun/hypernym pair by examining the appearances of that noun in the source text and verifying that the hypernym was correct for the predominant sense of the noun Table presents the results ... children into children of the parent instead R e s u l t s and evaluation There are 20,014 leaves (nouns) and 654 internal nodes in the final tree (reduced from 20,013 internal nodes in the uncompressed...
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Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx
... are located in the central domain of each subunit, in the interface between the two dimers comprising the tetrameric structure [29] This domain contains five cysteine residues, two of them (Cys35 ... forming a disulfide bond which may be necessary for the tetrameric state of the enzyme In addition, Cys69 is involved in the correct folding of the monomer, supporting the establishment of the ... Cys35 in rat liver MAT), 44 (Cys57 in rat liver), and 92 (Cys105 in rat liver) are found to remain invariable in most of the sequences aligned The cysteines at positions 33 and 47 are found in the...
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Báo cáo Y học: The plant S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase is located in both envelope and thylakoid chloroplast membranes pot
... possibly involved in the anchoring of the protein to the membranes This part of the protein is absent in the prokaryotic MgPIXMT From databank surveys, we detected highly similar proteins in other ... transformed with either the plasmid containing the inverted insert or containing soMGD1 cDNA, a control cDNA encoding a MGDG synthase from spinach (Fig 2A,B) These results demonstrate that the characterized ... determined by the TMPRED program They are indicated in white letters on black background The amino terminus end of the recombinant truncated proteins (D80-protein and D161-protein) is indicated by...
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Báo cáo khoa học: Localization of fluorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum pot
... content in the 100–600 kDa position increased marginally, indicating that the free capacity of the protein(s) in the cytoplasm was limited in binding further polymalate The results are in agreement ... or the overlay technique Only nuclei were stained, while other organelles remained dark The staining intensity depended on the amount of the probes injected, the phase of the cell cycle, and the ... blue indicates chromatin and the green shows polymalatase epitopes The red fluorescence appears together with the blue DAPI staining (Fig 1F) indicating the transfer of the probe to the nuclei These...
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Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx
... growth of the conditional strain in glucose medium (Figs 10A and S4), indicating that, although these proteins show sequence similarity in the C-terminal portion, divergences in the remaining sequence ... ProtA–Utp25p, further indicating that this protein is associated only with the early prerRNAs This is in accordance with the hypothesis of the involvement of Utp25p in the early cleavages of the 35S pre-rRNA, ... proteins, Nop1p, Nop58p, Nop56p and Snu13p [8] In addition to the core proteins, the U3 snoRNP is associated with other proteins specific for this snoRNP The first proteins to be identified in the...
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Báo cáo khoa học: Crystal structure and solution characterization of the activation domain of human methionine synthase doc
... A of Ile1126 in the AdoMet-binding region In the human structure, the longer a3 helix ends at the beginning of the extra five amino acids The 3102helix is oriented below the beginning of helix ... lm for the FMN domain, which is 10-fold lower than the apparent Kd (11.9 lm) for binding of the D963E ⁄ K1071N to the flavin-binding protein The presence of AdoMet in the fluorescence binding assays ... the exact nature of these interactions remains to be determined The region containing b3–b5, known as the b meander [14], contains a six amino acid insertion in the human structure (Fig 1A) The...
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Báo cáo khoa học: "Distant supervision for relation extraction without labeled data" docx
... Wikipedia articles in the training phase and 400,000 different articles in the testing phase For human evaluation experiments, all 1.8 million relation instances are used in training Again, we use 800,000 ... articles in the training phase and 400,000 different articles in the testing phase For all our experiments, we only extract relation instances that not appear in our training data, i.e., instances ... sentences are combined, creating a richer feature vector In the testing step, entities are again identified using the named entity tagger This time, every pair of entities appearing together in a sentence...
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Báo cáo Y học: Engineering and use of 32P-labeled human recombinant interleukin-11 for receptor binding studies docx
... Verschueren) were maintained in DMEM containing 10% fetal bovine serum and mM glutamine All cell lines were maintained at 37 °C and 5% CO2 IL-11 bioassay IL-11 activity was measured using the IL-11-dependent ... between total binding (r) and nonspeci®c binding (m) Nonspeci®c binding represents the binding in the presence of excess unlabeled FPDIL-11 Values were the means of triplicates from two independent ... cultivated in ¯at-bottom microwell plates containing ´ 103 cells per well in the presence of serial dilutions of the cytokine After days of culture, the number of surviving cells was determined by...
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BÁO CÁO " NGHIÊN CỨU XÁC ĐỊNH NHU CẦU METHIONINE TRONG THỨC ĂN CỦA CÁ TRA (Pangasianodon hypophthalmus) " doc
... ăn hàm lượng methionine 32,9 g/kg protein, cao có ý nghĩa với mức methionine thấp (từ 11,9 đến 22,4 g/kg protein) (p
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