analysis of fluorescent protein expressing cells by flow cytometry

Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps

... distribution of healthy cells is approximated by Correlation of fluorescence and cell size Regardless of the presence of fluorochromes, every cell emits light when it is excited by a laser - ... complete work flow from raw flow cytometry data to a list of genes that are components of or interact with the cellular process of interest Procedures (methodologic recommendations as well as software) ... contained cells transfected with a different overexpression construct Along with the phenotype of interest, the amount of overexpression of the respective proteins was recorded via a fluorescent...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Danh mục: Báo cáo khoa học

... Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae ... as fluorescent dye allowing determination of the threshold cycle at which exponential amplification of PCR products begins Specificities of amplicons were confirmed by melting curve analyses by ... 6-carboxyfluorescein by the esterases of living cells to label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5...

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Báo cáo y học: " Targeted infection of HIV-1 Env expressing cells by HIV(CD4/CXCR4) vectors reveals a potential new rationale for HIV-1 mediated down-modulation of CD4" doc

Danh mục: Báo cáo khoa học

... determined by p24 ELISA Panel B: Time course of cell adhesion by HIV particles Binding of HIV(CD4) particles to Env+ cells (ᮀ), Env- cells (●), or adhesion of HIV(w/o CD4) particles to Env+ cells ... numbers (104 to × 104) of Env- BJAB cells, the parental cells of TF228 As shown in Figure 6, addition of increasing numbers of Env- cells dramatically decreased the number of neomycin resistant ... high At a ratio of Env+ to Env- cells, the transduction of Env+ cells was inhibited by over ninety percent (Figure 6) HIV-1 is a cytolytic retrovirus and the number of cells expressing HIV-1...

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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"

Danh mục: Y học thưởng thức

... variants (TV) of exon of the hCDC4 gene Arrows mark the position of the detected mutation in the 5’ UTR of Exon 1-TV3 and the new intronic SNP downstream of exon 10 (B) Chromatograph of the mutation ... exchange of nucleotide (nt) 108 of the exon sequence (Figure 1B) Being located in an untranslated region of the exon, the mutation has no consequence for the hCDC4 protein Sequence analysis of hCDC4 ... gels Results Mutational analysis of hCDC4 in AML In this study the common exons to 11 and the three known transcript variants of exon of hCDC4 (Figure 1A) were analyzed by direct DNA sequencing...

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Báo cáo y học: "ene expression analysis of human red blood cells"

Danh mục: Y học thưởng thức

... proposed A part of human erythrocytic proteins were identified 6, 7, 8, The number of genomic studies of RBCs is at present limited and represented either by micronucleus assay as a marker of classification ... of human RBCs The purity of erythrocyte fraction achieved 99,99997% and was confirmed by Pappenheim staining of blood slides, flow cytometry (MÖLAB, Germany) and FACS analysis (Cytomics FC 500 ... (Table 1) Table The purity of human RBCs fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.*...

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a discourse analysis of opening and closing speeches by masters of ceremonyon reality television showsin american english versus vietnames

Danh mục: Khoa học xã hội

... substitution of a noun as head of a noun phrase by one or ones, or the substitution of a whole phrase by the same 15 - Verbal substitution is by means of “Do” “Do” operates as head of a verbal ... Concept of Discourse Analysis The notion of discourse analysis is defined in many ways: Stubbs (1983:1) defined “discourse analysis as (a) concerned with language use beyond the boundaries of a ... the primary interest of discourse analysis in this study is to give an account of how forms of language are used in making speeches on the reality TV shows The analysis of discourse here would...

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Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx

Danh mục: Báo cáo khoa học

... were selected MALDI-TOF/TOF In addition to LC-MS ⁄ MS analysis, we also completed TOF ⁄ TOF analysis of selected peaks to conﬁrm structure using a MDS Sciex 4800 MALDI-TOF ⁄ TOFÔ instrument (Applied ... patterns Glycan analysis of cRBCs Analysis of cRBC glycans by 1H-NMR To determine the most relevant glycan sequences for each composition, we completed additional MS and NMR-based analysis of the cRBC ... species at m ⁄ z of 2792.2 and 3083.8 by the release of one and two sialic acids, respectively Quantitative 1H-NMR analysis of this sample also indicates the presence of a mixture of both a2 ﬁ and...

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Tài liệu Báo cáo khoa học: Efﬁcient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf

Danh mục: Báo cáo khoa học

... possibility of a saturable mechanism of entry (Fig 1A); identical results were obtained with a control ODN (not shown) Examination of the cells by light microscopy showed that untreated cells, cells ... and 48 h (Fig 1B); after 72 h, the amount of dead cells and debris made it difﬁcult to count dead cells Analysis by ﬂow cytometry clearly showed the cells that had incorporated hpST3dODN (FITC ... activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells with hpST3dODN was analysed in SW480 cells transfected...

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Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate speciﬁcity doc

Danh mục: Báo cáo khoa học

... loose packing of the protein tetramers in the crystal cell, which leaves a large amount of empty space, 80% of the volume, ﬁlled with solvent The alignment of the amino acid sequence of HP1287 ... reminiscent of that of human heme oxygenase-1 [13] In the present study, we present the crystal structure of the HP1287 gene product as well as one of its mutants (F47Y) and discuss the in vivo role of ... identity and 51% similarity to the TenA protein from B subtilis The 3D structure of the monomer is quite similar to that of the other members of the TenA family of known structure: twelve a-helices,...

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An Analysis of Small Business and Jobs by Brian Headd Office of Advocacy pot

Danh mục: Quản trị kinh doanh

... Administration, Office of Advocacy from data provided by the U.S Department of Commerce, Census Bureau, Business Dynamics Statistics Using a different type of analysis defining firms by just their ... Administration, Office of Advocacy, from data provided by the U.S Department of Labor, Bureau of Labor Statistics, Business Employment Dynamics Firm Size Much of the preceding discussion of job creation ... Administration, Office of Advocacy, from data provided by U.S Department of Commerce, Census Bureau, Statistics of U.S Businesses which covers the private-sector Small businesses’ share of private-sector...

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Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide. ppt

Danh mục: Báo cáo khoa học

... degree of Nat5 inhibition observed in the cells The cell cycle disruption induced by the downregulation of Nat5 expression is induced by the activation of p53 that induces an increase of p21 ... absence of a speciﬁc marker One of the general features of tumour cells consists of changes in their cell surface, which can be also reﬂected in the glycosylation pattern of their secreted glycoproteins ... Bickle2 Laboratory of Dr Ettrich, Institute of Physical Biology of USB and Institute of Landscape Ecology of AS CR, Nove Hrady, Czech Republic, 2Laboratory of Dr Bickle, Department of Microbiology,...

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Economic Analysis of the House Budget Resolution by the Center for Data Analysis at The Heritage Foundation pot

Danh mục: Cao đẳng - Đại học

... employment grew by an annual average of 1.6 million jobs above the CBO alternative budget baseline Total employment grew by an average of 1.3 million jobs, which indicates shrinkage of public-sector ... Treasury notes fell by an average of 37.6 basis points The Consumer Price Index was virtually unchanged • Household Net Worth: The net worth of households increases by an annual average of $564 billion ... dollars spent by the federal government over this ten-year period o Non-Defense Discretionary Purchases fall by an average of $118 billion per year o Defense Purchases fall by an average of $128 billion...

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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot

Danh mục: Hóa học - Dầu khí

... cytometry (Beckman Coulter, USA) Cells were stained with Giemsa to visualize the morphology of erythroid cells Phosphoprotein analysis Phosphoproteins of HSCs/CD34+ cells isolated from HbE/ b-thalassemia ... types of HSCs and erythroid cells Cell surface markers were detected with mouse anti-CD 34, 71, 45, and glycophorin A antibodies conjugated with fluorescent dye followed by analysis using flow cytometry ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...

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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx

Danh mục: Hóa học - Dầu khí

... cytometry (Beckman Coulter, USA) Cells were stained with Giemsa to visualize the morphology of erythroid cells Phosphoprotein analysis Phosphoproteins of HSCs/CD34+ cells isolated from HbE/ b-thalassemia ... types of HSCs and erythroid cells Cell surface markers were detected with mouse anti-CD 34, 71, 45, and glycophorin A antibodies conjugated with fluorescent dye followed by analysis using flow cytometry ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...

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Báo cáo toán học: " Enhancement of the photoelectric performance of dye-sensitized solar cells by using Ag-doped TiO2 nanofiber in TiO2 nanoparticle film as an electrode" pot

Danh mục: Toán học

... reduction of the electron transit time can explain the increment of Jsc by the addition of ATN Conclusions In conclusion, TN and ATN were added into the TiO2 film of DSSCs An enhanced η of 129% ... Characterization and morphology of prepared titanium dioxide nanofibers by electrospinning J Nanosci Nanotechnol 2010, 10:3402-3405 17 Li D, Xia Y: Fabrication of titania nanofibers by electrospinning Nano ... study has discussed the electrochemical properties of the TN-added cells or the ATN-added cells by photocurrent-voltage curves Experiment Preparation of TN and ATN TN was fabricated using the electrospinning...

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Báo cáo hóa học: " Design and Analysis of Nanotube-Based Memory Cells" ppt

Danh mục: Hóa học - Dầu khí

... and DRAM cells In addition, the continuum model developed in this paper will help to study feasibility of large nanotube-based memory cells in practical applications Fabrication of arrays of nanotube ... employed as memory cells We also develop a continuum model in this paper to analyze the proposed NEMS memory cell design Design of Memory Cells Figure illustrates a simple example of the proposed ... the fabrication of regular arrays of CNTs suitable for use in integrated circuits has been demonstrated Extension of these or other techniques for fabricating regular arrays of DWNTs will be...

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Báo cáo vật lý: "Analysis of Trace Elements in Teeth by ICP-MS: Implications for Caries" pptx

Danh mục: Báo cáo khoa học

... pulps of carious teeth than in those of healthy teeth, and the mean concentrations of Mg, Cd, Ba and Pb were higher in the pulps of carious teeth than in those of healthy teeth (Fig 3) Journal of ... Optimisation 2.2 950 W Maximum ion intensity of 10 ppt of 89Y Collection and Preparation of Primary and Permanent Teeth A total of 64 primary teeth of all types (incisors, canines and molars) ... components of the amalgam (Cu, Zn and Hg) diffuse into the structures of the teeth Curson and Crocker20 determined the concentrations of Zn and Pb in the pulp of the teeth Journal of Physical...

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Caldesmon regulates the motility of vascular smooth muscle cells by modulating the actin cytoskeleton stability doc

Danh mục: Báo cáo khoa học

... untransfected cells, as seen in the control cells (Fig 1-d,e) Effect of phosphomimetic mutation of CaD on the motility of A7r5 cells To determine the effect of phosphomimetic mutation of CaD on the ... migration activity of mutated A7r5 cells clearly was not owing to inhibited contractility State of phosphorylation of CaD mutants by Western blot analysis The fact that cells expressing the A1A2 ... A1234 cells and the Page of 12 control cells Apparently both MAPK and PAK contribute to the motility of A7r5 cells and these effects are comparable and additive Effect of phosphomimetic mutation of...

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báo cáo khoa học: "Multi-level analysis of electronic health record adoption by health care professionals: A study protocol" potx

Danh mục: Báo cáo khoa học

... constructively critical review of the state -of- the-science Journal of Organizational Behavior 2004, 25:147-173 51 Damanpour F: Organizational innovation: A meta -analysis of effects of determinants and moderators ... adoption of information systems to various degrees Nevertheless, to the best of our knowledge, possible interactions between factors influencing EHR adoption by specific groups of professionals ... norms are composed by normative and role beliefs Normative beliefs consist of the internalisation by an individual of referent people or groups' opinion about the realisation of the behaviour,...

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