infections cell to cell transfer and transmission by flow cytometry
Báo cáo y học: "Enrichment of intersubtype HIV-1 recombinants in a dual infection system using HIV-1 strain-specific siRNAs" pps
... inhibition was overcome by dual infection We next examined the prolonged inhibitory effect of siRNAs on both mono- and dual -infections (Figure 3A) Monoinfections with v120-A and v126-D in the absence ... mono- and dual -infections with HIV-1 v120-A and v126-D (A) Schematic illustration of the monoinfections or dual infections with or without siRNA treatment for a first round on U87.CD4.CXCR4 cells ... dual infections [12,13,17] Here, we used subtype-specific primers (ESD1 and ESA2) to amplify envelope fragment from 5’ v126-D/3’ v120-A env recombinants and conserved primers (EAD1 and EAD2) to...
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The effects of clay a m e n d m e n t and composting on metal speciation in digested sludge liang qiao
... in the sludge was shifted to more available forms (Fig 6) This may be caused by changes due to storage of the sludge and to moisture change The pH of sludge after storage dropped suggesting that ... 96°C (reducible or bound to oxides fraction); 30% H202 at pH and 85°C/3.2 M NH4OAc (bound to organic fraction) extractions and acid digestion by concentrated HNO3, HC104 and HC1 (residue fraction) ... fraction and finally Mn oxides in the sludge 100 *535 451 434 515 compost; the clay plasma and the sandstone oxyhydroxides sorb little Cu The composting process reduced the organic bound Cu and transferred...
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Tài liệu Finance and Economics Discussion Series Divisions of Research & Statistics and Monetary Affairs Federal Reserve Board, Washington, D.C.: Interest Rate Risk and Bank Equity Valuations doc
... (bottom panel).12 To examine the reaction of bank stock prices to the two interest rate surprises, we rely on the Trade and Quote (TAQ) intraday stock price data collected and published by the ... As emphasized, for example, by Gorton and Rosen [1995], Choi and Elyasiani [1997] and Purnanandam [2007], banks can, and in many cases do, actively use derivatives to alter their 21 interest rate ... Bernanke and Kuttner [2005] for a thorough discussion.3 Motivated by the conventional notion of See, for example, DeYoung and Roland [2001] and Stiroh [2004] In what follows, we refer to both BHCs and...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... nm, the temperature was shifted to 15 °C and the cells were grown to maximum cell density (24– 48 h) without induction Cells were harvested by centrifugation at °C and 6000 g for 10 (Avanti J-25 ... activation of PLDa2 by calcium ions is due to the binding of calcium ions to the enzyme, and not (or not only) to better structuring of the substrate aggregates From both the CD and the activity ... JA-10 rotor), and stored at ) 20 °C Purification of PLDa2 The pellet from a 1.2 L culture was suspended in 37 mL of buffer (30 mm Pipes, pH 6.2, 10 mm EDTA), and the cells were disrupted by high-pressure...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... among pollen and food allergic patients [18] Finally and recently, a 46 kDa allergen has been reported and its internal peptide sequences shown to have some sequence similarity to cytochrome c ... BGP by two chromatographic steps using a CM-TSK column and reverse phase HPLC When fraction AS1 in starting buffer was applied to the CM-TSK column, one major peak (C1) and two minor peaks (C2 and ... the amino acids at positions and 13 were L To define the oligosaccharides present, N-glycans released sequentially by trypsin digestion and glyco6222 Fig MALDI-TOF and PSD analyses of the glycopeptide...
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Báo cáo Y học: A polymer with a backbone of 3-deoxy-D-glycero -D-galacto -non-2ulopyranosonic acid, a teichuronic acid, and a b-glucosylated ribitol teichoic acid in the cell wall of plant pathogenic Streptomyces sp. VKM Ac-2124 pdf
... d100.2–103.6 belonged to the protonated anomeric carbon atoms, while the fifth signal of low intensity at d97.6 belonged to the nonprotonated carbon atom, presumably, to the anomeric atom C(2) of an ... ribitol mono- and bisphosphates were identified as alkaline hydrolysis products Acid hydrolysis afforded ribitol monophosphates and bisphosphates, anhydroribitol phosphate, anhydroribitol, ribitol, ... esters and to purify ribitol teichoic acid (20 VÆcm)1, h) Paper chromatography was performed in a pyridine-benzene-butanol-water (3 : : : 3, v/v/v/v) solvent system to separate ribitol and glucose...
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Future R&D Environments A Report for the National Institute of Standards and Technology potx
... the food and agriculture sectors Both computer and information science and technology and biological sci- EXECUTIVE SUMMARY ence and engineering will be strongly influenced by pull factors because ... next decade In particular, progress in understanding anergy—the process by which cytotoxic cells can be made tolerant to, rather than activated by, specific antigenic signals— offers, for the ... individual, to model that individual’s biological responses to a drug or a combination of drugs, and thereby to customize drug therapies to an individual, reducing the uncertainties and side effects...
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Asthma WellnessKeeping Children with Asthma in School and Learning Liability & Litigation: A Legal Primer Asthma & Indoor Air Quality (IAQ)Asthma Management, Policies and Procedures.St r a i g h tBy Paul D. Houstont a l kIn School and Healthy: docx
... administrators stepped forward to advocate that school districts provide free and reduced breakfast and lunch programs Now it’s time for us to advocate for improved indoor air quality and policies and ... sleep, ability to concentrate, memory, and participation and can cause disruption to learning through repeated trips from the classroom to the school nurse to access medication (up to four times ... ensure safe and immediate access to prescribed medications, and use standard emergency protocols for students in respiratory distress If you need to send a letter to a physician regarding an asthma...
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bailin d., love a. cosmology in gauge field theory and string theory
... the form of protons However, eventually the electrons and protons combine into neutral atoms (This is referred to as ‘recombination’.) Thereafter, photons decouple from matter and evolve at a ... small and λ º e4 , then λ4 is negligible compared to e4 and B simplifies to 3e4 (2.65) B 64π With the mass-squared matrices of (2.40) and (2.41) for the (shifted) scalar and vector fields, and not ... LTEX by Text Text Limited, Torquay, Devon Printed in the UK by MPG Books Ltd, Bodmin, Cornwall Copyright © 2004 IOP Publishing Ltd To Eva Bailin and the memory of William Bailin (1911–1994) and To...
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báo cáo hóa học: " Methyl salicylate 2-O-b-D-lactoside, a novel salicylic acid analogue, acts as an antiinflammatory agent on microglia and astrocytes" docx
... penicillin, and 0.1 mg/ml streptomycin; and were found to be of 95% purity as determined by immunocytochemical staining with ox42 and anti-glial fibrillary acidic protein (GFAP) antibody To investigate ... of pro-inflammatory cytokines (IL-6, IL-1b, and TNF-a) and the expression of inflammation-related proteins (iNOS, COX-1, and COX-2) in LPS-activated microglia and astrocytes, and we explored ... inhibitory effect of DL0309 on NO/iNOS induced by LPS in glial cells Cells were pre-treated for h with the indicated concentrations (μM) of DL0309, and then stimulated by LPS (0.5 μg/ml) for 24 h Cell...
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Báo cáo hóa học: " Research Article Efficient and Precise Processing for Squinted Spotlight SAR Through a Modified Stolt Mapping" doc
... interpolation is limited to four (imposed by the used programming language) and an oversampling factor of has been used to enhance the quality of the interpolation step On Figure the contours of magnitude ... separation with respect to high squint angles to alleviate the interpolation step associated to the Stolt mapping As an immediate consequence, for a given interpolation accuracy and thus efficiency of ... leads to a limited skewing and shifting of the spectrum after the Stolt mapping, thus resulting in better performances regarding the overall resolution and the memory efficiency On the other hand...
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Báo cáo khoa học: "Analysis of growth and light interception of balsam fir and white birch saplings following precommercial thinning D Pothier A" doc
... referred to as thinning shock (Harrington and Reukema, 1983) However, the thinned white birches were still able to respond to treatment by decreasing SLA during both summers (table II) and this ... resistance to water flow from roots to leaves and thus help trees acclimate to a higher rate of transpiration Thinned and control balsam fir no longshowed different sapwood area growth to leaf area ... conversion factors Furthermore, the basal area growth for 1988 and 1989 was calculated for each of the crown sections and its percentage in relation to the total basal area was calculated This factor was...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
... N-hydroxycinnamoyl/benzoyltransferase group [51] and are closely related to their tobacco and tomato orthologues The hydroxycinnamoyltransferase activity of the enzyme and its involvement in PP ... described in sweet potato [26] Although the globe artichoke HCT sequence is similar to that of tobacco HCT, its activity is more closely related to that of tobacco and tomato HQT, in showing a ... dithiothreitol, 100 μM of chlorogenic acid and 100 μM CoA Reactions were incubated at 30°C for 30 min, stopped by the addition of 20 μl of acetonitrile/HCl (99:1) and products were analysed by reverse-phase...
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Báo cáo y học: "Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP)" pps
... fragments were F1 = to 60, F2 = to 90, F3 = to 120, F4 = to 170, F5 = 169 to 244, F6 = 129 to 244 and F7 = 83 to 244 The deduced molecular masses for these recombinant peptides F1 to F7 were 6.1, ... minutes to remove bacterial RNA and DNA and to reduce the viscosity of the cell lysate The lysate was then centrifuged at 10,000 g for 20 at 4°C The supernatant was collected and added to mL TALON ... The cell pellet from mL of culture was resuspended in 100 uL of reducing SDS sample buffer and subjected to SDS-PAGE to evaluate expression levels Cells were harvested from the culture samples by...
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Báo cáo sinh học: " SNP mapping of QTL affecting growth and fatness on chicken GGA1" pptx
... FLIPS and FIXED were used to evaluate the order of markers along the chromosome and to estimate the map distance between markers rs1384934 4(M4) and rs15551556 (M28) could not be assigned to the ... Murani E., Schellander K., Schwerin M., Wimmers K., Identification of functional candidate genes for body composition by expression analyses and evidencing impact by association analysis and mapping, ... curves for 35 d BW and 42 d BW almost overlapped, and two QTL curves for 49 d BW and 70 d BW almost overlapped too (Fig 2) The additive effects of these QTL were both positive, and the dominant...
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Báo cáo y học: "Strict glycaemic control in patients hospitalised in a mixed medical and surgical intensive care unit: a randomised clinical trial"
... 0.0006 and 0.0151 In both analyses they recommended to continue the trial Randomisation Patients were randomly assigned into study groups with a 1:1 ratio according to a computer-generated random ... patient had insulin infusion, and every four and six hours if no insulin was required according to the algorithm A protocol (see additional data files and 2), managed by the ICU nurses, was used ... according to the treating physician's recommendations and protocols were stopped We registered every patient's age, sex, body mass index, diabetes history, type of diabetes treatment, previous infections, ...
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Báo cáo y học: "Spinal Intramedullary Cysticercosis: A Case Report and Literature Review"
... lymphocytic pleocytosis and eosinophilia7 Cysticercal antibodies found in CSF either by ELISA or in serum by enzyme-linked immunoelectric transfer bolt assay have good sensitivity and specificity ... hypointense on T1WI and hyperintense on T2WI Figure Photomicrographs of the histological specimen showing the cysticercosis cyst wall with neutrophile granulocyte.lymphocyte and necrosis cell (H&E×100) ... course from a week to 10 years20 The common clinical manifestations included pain, paraparesis, spasticity, bowel and bladder incontinence, and sexual dysfunction1,20 However, inflammatory reaction...
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Báo cáo y học: "Mirror-Image Arachnoid Cysts in a Pair of Monozygotic Twins: A Case Report and Review of the Literature"
... genetic contribution to AC Mirror-imaging of ACs may be more common than previously reported We also consider that it is mandatory to monitor the counterpart of the symptomatic patient with AC ... SPG4 and SOX2 gene might be linked to AC21-23 Consequently, further sequencing of those candidate genes is warranted Secondly, most AC patients remain asymptomatic through their whole life, and ... consider that it is essential to systematically monitor the counterpart of the symptomatic patient, irrespective of the absence of symptoms 404 At last, Twin A was admitted to our department with a...
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Viewing a WSDL File and Testing a Web Service
... name="RetrieveCustomers"> ... been converted to plus (+) characters You'll need to scroll down the page to see the other customers Figure 17.6: Running the RetrieveCustomers() method with a whereClause of CustomerID IS NOT ... name="CustomersSoap"> ...
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Configuring a Stub Area and a Totally Stubby Area
... Capetown can use the default route to its ABR for all nonlocal area traffic, you decide to filter Type and Type interarea routes from Area To this, you must configure Area as a totally stubby ... You must also configure Capetown: Capetown(config)#router ospf Capetown(config-router)#area stub Verify that Area is a stub by issuing the show ip ospf command: CapeTown#show ip ospf Routing Process ... creating a totally stubby area Only the ABR needs the additional configuration The role of Area internal routers has not changed Return to Capetown and check its routing table: Capetown#show ip...
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