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5 kỹ thuật nuôi một số loài cá nước ngọt

Báo cáo y học:

Báo cáo y học: "Modulation of interleukin-1b-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts" docx

Báo cáo khoa học

... 50 40 30 20 10 Ctrl IL1- + TNF- B IL-1RA (pg/ml) - IDR peptide * 450 400 + IDR-1002 + IDR-1 350 300 250 200 150 100 50 Ctrl IL-1 Fold Change Relative to un-stimulated cells normalized to C 25 ... (SP6001 25) represses c-Jun activation, DNA-binding and PMA-inducible Turner-Brannen et al Arthritis Research & Therapy 2011, 13:R129 http://arthritis-research.com/content/13/4/R129 53 54 55 56 57 ... http://arthritis-research.com/content/13/4/R129 Millions A NS 4 .5 * - peptides Page 10 of 14 B + IDR-1002 + IDR-1 - NF- B p50 NF- B activation 3 .5 - -actin 2 .5 Ctrl IL-1 1 .5 (i) (ii) IL1 + IDR-1002 IL-1 + IDR-1 0 .5 Ctrl IL-1 Figure Monitoring...
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Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx

Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx

Báo cáo khoa học

... of NF-kappaB p 65 on serine 53 6 is mediated through FEBS Journal 278 (2011) 862–876 ª 2011 The Authors Journal compilation ª 2011 FEBS H Wajant and P Scheurich 52 53 54 55 56 57 58 59 60 61 62 63 ... Bone marrow macrophages TRAF5) ⁄ ) MEFs TRAF5) ⁄ ) Bone marrow macrophages TRAF2) ⁄ ) TRAF5) ⁄ ) MEFs TRAF2) ⁄ ) TRAF5) ⁄ ) MEFs TRAF2) ⁄ ) TRAF5) ⁄ ) MEFs TRAF2) ⁄ ) TRAF5) ⁄ ) MEFs IKK activity ... Nature 423, 655 – 659 Hoberg JE, Yeung F & Mayo MW (2004) SMRT derepression by the IkappaB kinase alpha: a prerequisite to NF-kappaB transcription and survival Mol Cell 16, 2 45 255 Shembade N,...
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báo cáo hóa học:

báo cáo hóa học: " Aspirin-triggered lipoxin A4 attenuates LPSinduced pro-inflammatory responses by inhibiting activation of NF-B and MAPKs in BV-2 microglial cells" pptx

Hóa học - Dầu khí

... intestinal Wang et al Journal of Neuroinflammation 2011, 8: 95 http://www.jneuroinflammation.com/content/8/1/ 95 44 45 46 47 48 49 50 51 52 53 54 Page 12 of 12 epithelial cells through inhibition of ... (Invitrogen): 5 CAGCTGGGCTGTACAAACCTT-3’ and 5 - CATTGGAAGTGAAGCGTTTCG-3’, which amplify the 95 bp product for iNOS; 5 -CAACCAACAAGTGATATTCTCCATG-3’ and 5 - GATCCACACTCTCCAGCTGCA-3’, which amplify the 152 ... buffer containing 2 .5% glycerol, mM Wang et al Journal of Neuroinflammation 2011, 8: 95 http://www.jneuroinflammation.com/content/8/1/ 95 Page of 12 MgCl2, 50 ng/μl poly (dI-dC), 0. 05% Nonidet P-40,...
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Báo cáo y học:

Báo cáo y học: "Differential recovery of glycosaminoglycan after IL-1-induced degradation of bovine articular cartilage depends on degree of degradation" pot

Báo cáo khoa học

... times of 25, 75, 1 25, 1 75, 2 75, 3 75, 4 75, 600, 900, and 1800 ms For inversion recovery measurements, images were acquired with nine inversion delays of 16.7, 33.3, 50 , 66.7, 100, 150 , 250 , 400, ... 267:3691-36 95 Bashir A, Gray ML, Boutin RD, Burstein D: Glycosaminoglycan in articular cartilage: in vivo assessment with delayed Gd(DTPA)(2-)-enhanced MR imaging Radiology 1997, 2 05: 55 1 -55 8 Nerucci ... Matrix Biol 1998, 17 :51 3 -52 3 Correspondence ML Gray, Massachusetts Institute of Technology, Harvard–MIT Division of Health Sciences and Technology, 45 Carleton Street, E 25- 519, Cambridge, MA 02142,...
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Báo cáo y học:

Báo cáo y học: "Melanocortin peptides inhibit urate crystal-induced activation of phagocytic cells" ppsx

Báo cáo khoa học

... crystals To measure CL, 2 .5 × 1 05 neutrophils were mixed in 3-mL polystyrene vials with × 10 -5 M luminol in a final volume of 700 μL The vials were placed in a Luminometer 1 251 (LKB Wallac, Turku, ... 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Vol 11 No Capsoni et al gen-induced lymphocyte proliferation in humans independently of melanocortin receptor gene status J Immunol 20 05, 1 75: 4806-4813 ... 78 :55 9 -56 9 Serhan CN, Lundberg U, Weissmann G, Samuelsson B: Formation of leukotrienes and hydroxy acids by human neutrophils and platelets exposed to monosodium urate Prostaglandins 1984, 27 :56 3 -58 1...
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Involvement of p63 in the herpes simplex virus-1-induced demise of corneal cells Research ppt

Involvement of p63 in the herpes simplex virus-1-induced demise of corneal cells Research ppt

Báo cáo khoa học

... 20 05, 17:217-229 55 Meulmeester E, Maurice MM, Boutell C: Loss of HAUSP-mediated deubiquitination contributes to DNA damage-induced destabilization of Hdmx and Hdm2 Mol Cell 20 05, 18 :56 5 -57 6 ... 20 05, 102: 952 3- 952 8 Robertson DM, Ho SI, Cavanagh DH: Characterization of ΔNp63 isoforms in normal cornea and telomerase-immortalized human corneal epithelial cells Exp Eye Res 2008, 86 :57 6 -58 5 ... protein BAX J Biol Chem 1998, 273:1193-11936 50 Dietrich JB: Apoptosis and anti-apoptosis genes in the Bcl-2 family Arch Physiol Biochem 1997, 1 05: 1 25- 1 35 51 Schlottmann K, Schölmerich J: BCL-2 family...
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Báo cáo y học:

Báo cáo y học: "Involvement of lipid rafts in adhesion-induced activation of Met and EGF" pdf

Báo cáo khoa học

... expression of a mutant epidermal growth factor receptor in multiple human tumors Cancer Res 19 95, 55 :55 36 -55 39 Trusolino L, Comoglio PM: Scatter-factor and semaphorin receptors: cell signalling for ... Biol 2010, 20: 75- 79 14 Aratyn-Schaus Y, Gardel ML: Transient frictional slip between integrin and the ECM in focal adhesions under myosin II tension Curr Biol 2010, 20:11 45- 1 153 15 Yeung T, Georges ... Sinica, Taiwan To produce lentiviruses, HEK293T cells were co-transfected with 2. 25 µg pCMV-∆R8.91, 0. 25 µg pMD.G, and 2 .5 µg hairpin-pLKO.1 by TransIT-LT1 (Mirus Bio) After days, the medium containing...
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Báo cáo y học:

Báo cáo y học: "Selective killing of human immunodeficiency virus infected cells by non-nucleoside reverse transcriptase inhibitor-induced activation of HIV proteas" doc

Báo cáo khoa học

... + + - + 2 .5 pCHIV pCHIV.MAα 2μM LPV - + - + 2μM LPV 2.0 Gag.MAα Gag 50 37 Gag.MAα 50 37 25 25 CA 1 .5 1.0 0 .5 20 20 OD592 GagPol/ GagPol.MAα 75 100 75 MA* MA 0.0 αMA αCA αbeta-Gal 10 15 20 time ... Page of 14 GagPol 75 50 37 Gag 25 20 GFP intensity [%] C A CA B -1.0 -0 .5 0.0 0 .5 1.0 log [µM GW-678248] 100 50 -1 .5 n.s -1.0 -0 .5 0.0 0 .5 1.0 0 .5 1.0 log [µM EFV] n.s p=0.0 052 2.0x106 p=0.036 ... Jochmans et al Retrovirology 2010, 7:89 http://www.retrovirology.com/content/7/1/89 50 51 52 53 54 55 56 57 58 59 60 Page 14 of 14 membrane-targeting signal: analysis of its role in enhancement...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx

Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx

Báo cáo khoa học

... 350 – 355 45 Bokoch, G.M., Wang, Y., Bohl, B.P., Sells, M.A., Quilliam, L.A & Knaus, U.G (1996) Interaction of the Nck adapter protein with p21-activated kinase (PAK1) J Biol Chem 271, 257 46– 257 49 ... Cells were lysed in lysis buffer (50 mM Hepes pH 7 .5, 150 mM NaCl, 1% NP-40, 0 .5% sodium deoxycholate, 10 lgÆmL)1 leupeptin, mM phenylmethylsulfonyl fluoride, 2 .5 lgÆmL)1 aprotinin, mM sodium fluoride, ... 22748–22 755 53 Zhao, Z.S., Manser, E., Loo, T.H & Lim, L (2000) Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly Mol Cell Biol 20, 6 354 –6363 54 Moolenaar,...
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báo cáo hóa học:

báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf

Hóa học - Dầu khí

... of Neuroinflammation 2011, 8:102 http://www.jneuroinflammation.com/content/8/1/102 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 Page 13 of 13 therapeutic effect of palmitoylethanolamide Eur J ... 0 ,59 2++ 25, 277 ± 1,403** 16,621 1,4 65 16 621 ± 4 65+ + Cnr1-/contralateral 12,013 ± 0,489 13,223 ± 0,788 15, 909 2,327 15 909 ± 327 Ipsilateral 20,028 ± 1, 257 ## 21,341 ± 0, 958 ## 19, 657 1,120 19 657 ... ## vehicle vehicle 100nM 50 0nM M M 10 M % VCAM-1 expression EV referred to TME AEA TMEV (2x105pfu) C % VCAM-1 express sion referred to TMEV 350 0 3000 250 0 2000 150 0 1000 50 0 D 120 100 80 60 40...
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Báo cáo y học:

Báo cáo y học: "Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1β-induced NF-κB-mediated inflammation and apoptosis" potx

Báo cáo khoa học

... with 10 ng/ml IL-1β, 50 μM resveratrol, 50 μM curcumin, 50 μM resveratrol and 50 μM curcumin, or pre-treated with 50 μM resveratrol, 50 μM curcumin, or 50 μM resveratrol and 50 μM curcumin for ... ng/ml IL-1β, with 50 μM resveratrol, 50 μM curcumin, and 50 μM resveratrol and 50 μM curcumin, or pre-treated with 50 μM resveratrol, 50 μM curcumin, and 50 μM resveratrol and 50 μM curcumin for ... ng/ml IL-1β, 50 μM resveratrol, 50 μM curcumin, 50 μM resveratrol and 50 μM curcumin; alternatively they were pre-treated with 50 μM resveratrol, 50 μM curcumin, 50 μM resveratrol and 50 μM curcumin...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

Báo cáo khoa học

... DNA Nature 252 , 754 – 755 Bain J, McLauchlan H, Elliott M & Cohen P (2003) The specificities of protein kinase inhibitors: an update Biochem J 371, 199–204, doi:10.1042/BJ2002 153 5BJ2002 153 5 [pii] Bain ... selected for sequencing b4CH3SOH 52 2.34 y4y2-NH3 244.14 y1 147.11 CH3SOH y3- 401.22 CH3SOH 783.48 b4 58 6.33 344.20 b1 268.23 847.47 y5 57 9.26 b2 382.28 y4 4 65. 21 b5 700.37 b5- 848.48 CH3SOH 636.29 784.47 ... 38 39 40 41 42 43 44 45 conformations Protein Sci 2, 155 9– 157 3, doi: 10.1002/pro .55 60021003 Colombo S, Longhi R, Alcaro S, Ortuso F, Sprocati T, Flora A & Borgese N (20 05) N-myristoylation determines...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

Báo cáo khoa học

... GRASP 55 (GRASP 55- GFP) were obtained from F Barr and colleagues [ 35] GRASP55F in pCDNA3.1 Zeo+ contained a C-terminal FLAG tag Full-length GRASP 55, GRASP 55 PDZ1 (amino acids 1–107), GRASP 55 PDZ2 ... + VP16 MT1 FRR GAL4 GRASP 55 + VP16 MT1 HGT GAL4 GRASP 55 + VP16 MT1 PRR GAL4 GRASP 55 + VP16 MT1 LLY GAL4 GRASP 55 + VP16 MT1 CQR GAL4 GRASP 55 + VP16 MT1 SLL GAL4 GRASP 55 + VP16 MT1 DKV – + + * ... GAL4-GRASP 55 and furin, PC5/6B or PC7 ICDs were tested using the M2H system (B) Interaction between VP16-furin and GAL4-GRASP 55, GRASP 55 PDZ1 (GAL4P1), GRASP 55 PDZ2 (GAL4-P2) or GRASP 55 Region...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt

Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt

Báo cáo khoa học

... Fig 5A, phosphorylation of p38 and SAPK ⁄ JNK appeared at 30 min, and peaked at h (P = 0.001) and h (P = 0.0 05) , respectively (Fig 5B) However phosphorylation of ERK was not significant (Fig 5) ... transferred to a 4 350 A Shen et al polyvinylidine difluoride membrane (Millipore, Bedford, MA, USA) using a transfer apparatus at 0. 35 mA for 2 .5 h The membrane was then blocked with 5% nonfat milk ... (anti-mouse, : 50 0; BD Pharmingen, San Diego, CA, USA), ERK (anti-rabbit, : 50 0; Cell Signalling, Danvers, MA, USA), phosphorylated ERK (anti-rabbit, : 50 0; Cell Signal), p38 (anti-rabbit, : 50 0; Cell...
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Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx

Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx

Báo cáo khoa học

... tissue FEBS Journal 273 (2006) 4 754 –4769 ª 2006 The Authors Journal compilation ª 2006 FEBS 4767 Rac signaling towards TIMP-1 48 49 50 51 52 53 54 55 56 57 58 59 R Engers et al inhibitor of metalloproteinases-1 ... J 348, 52 5 53 0 Aspenstrom P (1999) Effectors for the Rho GTPases Curr Opin Cell Biol 11, 95 102 Bishop AL & Hall A (2000) Rho GTPases and their effector proteins Biochem J 348, 241– 255 Cotteret ... incubating protein A agarose–JNK complexes with 50 lL of 10 mm Tris ⁄ HCl buffer (pH 7 .5) , containing 150 mm NaCl, 10 mm MgCl2, 0 .5 mm dithiothreitol, 15 lm ATP, lCi [c-32P]ATP, and mgÆmL)1 glutathione...
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Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx

Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx

Báo cáo khoa học

... factor-stimulated tyrosine protein kinase activity J Biol Chem 259 , 255 3– 255 8 31 Downward, J., Waterfield, M.D & Parker, P.J (19 85) Autophosphorylation and protein kinase C phosphorylation of the ... 251 4– 251 7 55 Saxena, M., Williams, S., Tasken, K & Mustelin, T (1999) Crosstalk between cAMP-dependent kinase and MAP kinase through a protein tyrosine phosphatase Nat Cell Biol 1, 3 05 311 56 ... Weech, P.K & Gelb, M.H (1993) Slow- and tight-binding inhibitors of the 85- kDa human phospholipase A2 Biochemistry 32, 59 35 59 40 36 Chang, T.M., Chang, C.H., Wagner, D.R & Chey, W.Y (1999) Porcine...
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Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc

Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc

Báo cáo khoa học

... si-NOX2 + G P38 2 .5 p-P53 bcl-2 Protein level (fold of control) 2.0 P53 Control si-NOX2 Glucose si-NOX2 + Glucose ** p-P38 ** * 1 .5 †† †† † p-P53/β-actin P53/β-actin 1.0 0 .5 Caspase-3 0.0 β-actin ... 15. 0 Glucose * 1. 25 †† †† 0. 75 0 .50 0.00 p-PTEN/β-actin PTEN/β-actin F * 0.3 Insulin secretion (ng·mg–1 of cellular protein) ** ** 0. 25 p-Akt/Akt p-JNK/JNK protein level (fold of control) 1 .50 ... Antisense Sense Antisense 5 -UUCUCCGAACGUGUCACGUTT -3¢ 5 -ACGUGACACGUUCGGAGAATT-3¢ 5 -UGCCAGAGUCGGGAUUUCUTT-3¢ 5 -AGAAAUCCCGACUCUGGCATT-3¢ 5 -GTATAGAGCGTGCAGATAATT-3¢ 5 -UUAUCUGCACGCUCUAUACTT-3¢...
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Báo cáo khoa học: A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbb and removes Rev-erbb-induced inhibition of apoCIII transcription potx

Báo cáo khoa học: A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbb and removes Rev-erbb-induced inhibition of apoCIII transcription potx

Báo cáo khoa học

... melanogaster and Schizosaccharomyces pombe (GenBank accession nos XP _53 6 855 .2, Q8R331, NP_001017 056 .2, NP_001017401.1, NP_6088 95. 1 and NP _59 5833.1) Residues identical in all compared sequences are presented ... (nucleotides )1408 to +24) and pRL As illus- FEBS Journal 274 (2007) 53 70 53 81 ª 2007 The Authors Journal compilation ª 2007 FEBS 53 75 ZNHIT-1 as a cofactor of Rev-erbb J Wang et al Fig Recruitment ... vertebrate orthologue of E 75, Rev-erba and Rev-erbb Interestingly, they also identified E 75 as a potential gas sensor, and showed that CO or NO binds to E 75 to interfere with E 75- mediated repression...
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Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf

Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf

Báo cáo khoa học

... 0.064 0. 055 0. 053 0.040 > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > ± ± ± ± ± ± ± 0.0 15 0.011 0.006 0.004 0.004 0.002 0.003 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Table Selectivity ... Ab( 25 35) (1 lM) (5) Ab( 25 35) (5 lM) (6) Ab( 25 35) (20 lM), a majority of cells show obvious cytotoxicity (7) Ab( 25 35) (20 lM) and Ac-DEVD-CHO (2 lM) (8) Ab( 25 35) (20 lM) and compound ( 25 lM) ... 0.024 0.014 0.016 0.009 0.007 0.007 ± ± ± ± ± ± ± 0. 152 0.016 0.043 0. 155 0.023 0.0 25 0. 055 ± ± ± ± ± ± ± 0.284 0.127 0.089 0.3 15 0. 152 0.708 0. 155 cannot be removed Figure 2B shows that the caspase-3...
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc

Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc

Báo cáo khoa học

... using a 3– 15% (w ⁄ v) polyacrylamide gel (ATTO, Tokyo, Japan) Following electrophoresis, the gel was FEBS Journal 273 (2006) 55 08 55 16 ª 2006 The Authors Journal compilation ª 2006 FEBS 55 13 Human ... Caenorhabditis elegans J Neurochem 75, 1800–1809 FEBS Journal 273 (2006) 55 08 55 16 ª 2006 The Authors Journal compilation ª 2006 FEBS 55 15 Human GPCR activates nematode G protein M Minaba et al 39 Hwang ... [3H]QNB binding [34] These results suggest FEBS Journal 273 (2006) 55 08 55 16 ª 2006 The Authors Journal compilation ª 2006 FEBS 55 11 Human GPCR activates nematode G protein M Minaba et al Fig Alignment...
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