15 tiền văn sến 2004 thử nghiệm phân lập và tạo dòng nguyên bào sợi từ mô phôi thai người đại học khoa học tự nhiên tp hcm
KHÓA LUẬN tốt NGHIỆP CHUYÊN NGÀNH CÔNG NGHỆ SINH học KHẢO sát sự ẢNH HƯỞNG của DỊCH CHIẾT từ lá cây SỐNG đời (KALANCHOE PINNATA) lên QUÁ TRÌNH TĂNG SINH tế BÀO CÓ NGUỒN gốc từ DA
... ngày dòng 13 được phân lập từ tế bào đơn Năm 1962, George Todaro Howard Green tạo được dòng nguyên bào sợi phôi chuột (3T3) từ mô phôi của chuột Mus musculus Dòng tế bào được ứng dụng ... Saksela tạo dòng tế bào ung thư xương người (U-2 OS), có nguồn gốc từ dòng 2T được phân lập từ mô xương của bé gái 15 tuổi bị bệnh về xương Năm 1966, J.P Jacobs tạo dòng nguyên bào sợi ... định xét nghiệm hóa sinh miễn dịch 1.3 Đại cương về nguyên bào 1.3.1 Nguồn gốc [2, 13, 14] sợi Nguyên bào sợi có nguồn gốc từ trung mô, tồn hai dạng nguyên bào sợi (fibroblast) tế bào sợi...
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cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... and Rad53 (154 ,155 ) Chk1-dependent phosphorylation and stabilization of Pds1 (securin) in response to DNA damage results in inhibition of the metaphase-to-anaphase transition (156 ,157 ) In contrast, ... phosphorylation of Cdc25C on serine-216 Science 277, 150 1 150 5 150 Zeng, Y., Forbes, K C., Wu, Z., Moreno, S., Piwnica-Worms, H., and Enoch, T (1998) 150 Replication checkpoint requires phosphorylation ... 157 response to DNA damage is essential for its DNA damage checkpoint function Genes Dev 15, 1361–1372 158 Li, R and Murray, A W (1991) Feedback control of mitosis in budding yeast Cell 66, 158 ...
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methods in molecular biology, v.474. nanostructure design. methods and protocols, 2008, p.268
... Science 300, 625627 15 van Raaij MJ, Mitraki A (2004) Beta-structured viral fibres: assembly, structure and implications for materials design Curr Opin Solid State Mater Sci 8, 151 156 16 Yemini M, ... adenovirus binding to its human cellular receptor, CAR Science 286, 157 9158 3 18 Burmeister WP, Guilligay D, Cusack S, Wadell G, Arnberg N (2004) Crystal structure of species D adenovirus fiber knobs ... CrysChem plates (Hampton Research, Aliso Viejo, CA, http://www.hamptonresearch.com/) and CompactClover plates (Jena Bioscience, Jena, Germany, http://www.jenabioscience.com/); these are to be covered...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... (9:1) (v/v) A: Light petroleum (b.p 60-80oC); B: Ethanol, 0-7.5min, 0-7.5% B; 7.5 -15 min, 7.5% B isocratic; 15- 20 min, 7.5-20% B Acetonitrile: 1-propanol: water: 0.1% acetic acid (80:6:13.9:0.1) ... Acetonitrile: 1-propanol: water: acetic acid (80:6:13:0.1) (v/v); B: 100% Acetonitrile 0 -15 100% A isocratic; 15- 17 0-100% B; 17-19 100% B; 19-22 back to 100% A Spraying with a saturated solution ... preconditioned 10 g C-18 Sep-Pak cartridge (Phenomenex, USA) The Sep-Pak was flushed with 150 mL water and then 150 mL of 30% methanol to remove any remaining isoflavone glucosides The Sep-Pak was...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... presence of 40 U RNAse Out, 500 lM each dNTP and mM dithiothreitol (all reagents from Invitrogen) at 42 °C for 60 followed by an inactivation step at 70 °C for 15 As a control each sample was additionally ... dithiothreitol (all reagents from Invitrogen) at 42 °C for 60 followed by an inactivation step at 70 °C for 15 As a control each sample was additionally treated without reverse transcriptase PCR was performed ... membranes (Schleicher and Schuell) Membranes were soaked for h in TBST (20 mM Tris buffer, pH 7.5, 150 mM NaCl, 0.1% Tween 20) with 5% nonfat dry milk and subsequently incubated for h with anti-VRL-1...
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data analysis in molecular biology and evolution - xuhua xia
... NUCLEOTIDE SUBSTITUTIONS 115 I NTRODUCTION 115 USE DAMBE TO DOCUMENT EMPIRICAL SUBSTITUTION PATTERNS 118 2.1 Simple output 118 2.2 Detailed Output 119 13 PREAMBLE ... Substitutions 142 15 CASE STUDY 4: TRANSITION BIAS 147 I NTRODUCTION 147 GET SEQUENCE DATA 151 DATA ANALYSIS 152 3.1 Phylogeny reconstruction 152 3.2 Pair-wise ... software listings: http://evolution.genetics.washington.edu/phylip/software.html http://biosci.biosc.lsu.edu/general/software.html http://darwin.eeb.uconn.edu/molecular-evolution.html http://www.york.biosis.org/zrdocs/zoolinfo/software.htm...
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![progress in nucleic acid research and molecular biology [vol 67] - k. moldave (ap, 2001) ww](https://media.store123doc.com/images/document/14/y/bw/small_h4P4i1s5po.jpg)
progress in nucleic acid research and molecular biology [vol 67] - k. moldave (ap, 2001) ww
... gland, activation of iPLA2 by P2X7 agonists ATP or the ATP analog Bz-ATP [2',3'-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate] was observed (145) ATP or Bz-ATP induces the release of [3H]AA to the ... fimetion ATP could affect iPLA2fl function by at least three mechanisms First, ATP could bind to the ATP-binding domain of iPLA2fi to stimulate its intrinsie enzymatic aetivity Second, ATP could ... phospho-transfer reaction (46) Since iPLA2CJadsorbs to ATP-agarose and is desorbed by ATR it is possible that ATP directly binds to the ATP-binding domain of iPLA.2JL Sueh binding may modulate...
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Báo cáo hóa học: "GEOMETRIC AND HOMOTOPY THEORETIC METHODS IN NIELSEN COINCIDENCE THEORY" pdf
... (1972), no 1, 45–52 Ulrich Koschorke 15 [3] R F Brown, Wecken properties for manifolds, Nielsen Theory and Dynamical Systems (Mass, 1992), Contemp Math., vol 152 , American Mathematical Society, ... invariants, Fundamenta Mathematicae 184 (2004) , 187–203 [10] , Selfcoincidences in higher codimensions, Journal f¨ r die Reine und Angewandte Matheu [11] matik 576 (2004) , 1–10 , Nielsen coincidence ... Mathematik, 2003, http:/www.math.uni-siegen.de/topology/ ’ur publications.html , Nonstabilized Nielsen coincidence invariants and Hopf-Ganea homomorphisms, preprint, [13] 2005, http://www.math.uni-siegen.de/topology/publications.html...
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báo cáo khoa học: " Hypercalcemia and huge splenomegaly presenting in an elderly patient with B-cell non-Hodgkin’s lymphoma: a case report" potx
... 8.1*10/ml 4-10.8 × 103/ml Ca 13.3 8.5-10.3 mg/dl P Creatinin 3.5 1.9 2.5-4.5 mg/dl 0.5-1.2 mg/dl PTH 15 15-65 pg/ml LDH 936
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Báo cáo y học: "Text-mining and information-retrieval services for molecular biology" pdf
... abbreviation server http://bionlp.stanford.edu/abbreviation/ [35] AbGene Protein name tagger ftp://ftp.ncbi.nlm.nih.gov/pub/tanabe [29] ABNER Protein/Gene/DNA/RNA/cell tagger http://www.cs.wisc.edu/~bsettles/abner/ ... literature database and search http://www.scopus.com/scopus/home.url [93] Textpresso C elegans literature information retrieval and extraction tool http://www.textpresso.org/ [48] XplorMed Explores ... 18:1124-1132 AbGene [ftp://ftp.ncbi.nlm.nih.gov/pub/tanabe] Settles B: Biomedical named entity recognition using conditional random fields and rich feature sets Proc NLPBA/COLING 2004 2004 ABNER: a biomedical...
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COLLEGE STUDENTS PERCEPTIONS OF QUARANTINE AND SOCIAL DISTANCING METHODS IN THE EVENT OF AN INFLUENZA PANDEMIC
... reported by a number of studies (Blendon et al, 2004; Cava et al., 2005a; DiGiovanni et al., 2004; Gammon, 1998; Grazier, 1988; Hwaryluck et 12 al., 2004) Blendon et al (2006) asked the U.S general ... and emotional support both during and after the quarantine (Blendon et al., 2004; Cava et al., 2005a, DiGiovanni et al., 2004) If quarantined in a healthcare facility, health care workers could ... conducted by DiGiovanni et al (2004) in which they found that students and single people had greater difficulty in relying on or obtaining help from others DiGiovanni et al (2004) noted that during...
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Excluded volume effects in molecular biology and extracellular matrix biochemistry biophysical considerations and molecular modeling
... 6.9.2 An Evolving Facet of Crowding in Multi-Cellular Organisms: the ECM 15 6.9.3 Macromolecular Crowding in Extremophiles 15 6.9.4 Challenges for Quantitative Estimation of the degree of ‘Crowdedness’ ... The biological model should yield a readily measurable output The model should allow application of a range of crowder concentrations The output measurement of the model reaction should not be affected ... CT, Threshold Cycle ° C, degrees Celsius cDNA, Complementary DNA DLS, Dynamic Light Scattering dNTPs, Deoxy-ribose Nucleotide Triphosphates DxS, Dextran Sulfate ECM, Extracellular Matrix EVE, Excluded-Volume...
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siRNA, miRNA and their possible roles in molecular therapy of chronic human hepatitis b virus infection and hepatocellular carcinoma
... 147 150 Chapter VI Conclusion 6.1 Summary of Important Findings………………………………………… …… 6.2 Suggestions for Future Work………………………………………….… ….…… 153 155 5 REFERENCES………………………………………………………………………… 158 SUPPLEMENTARY ... Transcripts in PLC/PRF/5 and 2.2 .15 Cells……… 4.3.3 Effect of siRNA on HBV Transcripts in 2.2 .15 Cells…………………… …… 4.3.4 Effect of siRNA on Virion Production in 2.2 .15 Cells……………….….……… 4.3.5 The ... PLC/PRF/5 and 2.2 .15 cells………………………………………… 85 Table 4.3 Effects of siRNAs on the viability of PLC/PRF/5 and 2.2 .15 cells…………………………………………………… 98 Table 4.4 Gene expression in 2.2 .15 and PLC/PRF5...
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Application of knowledge discovery and data mining methods in livestock genomics for hypothesis generation and identification of biomarker candidates influencing meat quality traits in pigs
... http://www.animalgenome.org/ last accessed March 13, 2014 http://www.animalgenome.org/gbrowse/ last accessed March 14, 2014 http://www.animalgenome.org:8181/ last accessed March 14, 2014 http://www.animalgenome.org/cgi-bin/QTLdb/index ... 34 http://www.plantcyc.org/ last accessed April 7, 2014 http://mapman.gabipd.org/web/guest last accessed April 7, 2014 36 http://www.cls.zju.edu.cn/pair/ last accessed April 7, 2014 37 http://www.genemania.org/ ... there http://www.geneontology.org/GO.current.annotations.shtml last accessed March 6, 2014 http://www.kegg.jp/kegg-bin/search_pathway_text?map=bta&mode=1 last accessed March 6, 2014 http://www.kegg.jp/kegg-bin/search_pathway_text?map=ssc&mode=1...
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Purification and cloning of PCR products
... in the presence of, for example dGTP, then the ends of the vector will 126 PCR 3' 5' PCR-amplified product CCCCCC 5' 3' Terminal transferase CCCCCC +dCTP + dGTP 3' Vector 5' GGGGGG 5' GGGGGG Vector ... polymerase, in the presence of the four dNTPs (see Protocol 6.1) This procedure results in the enzyme removing the unpaired terminal nucleotide, but the presence of the dNTPs means that if the enzyme removes ... AGT TTG TAC AAA AAA GCA GGC Recombine with Donor vector containing T-target sequence 3’ attP1 and attP2 sites Ensure the reading frame is maintained as shown for the triplet 5’GGG GAC CAC TTT...
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Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt
... containing 0.75 lL of a template, 150 pmol of a forward primer (A), 150 pmol of a reverse primer (B or C), · G-Taq buffer, 10 nmol each of dATP, dGTP, dCTP and dTTP, and 0.5 U G-Taq DNA Polymerase ... containing 0.5 lL of a template, 15 pmol of a forward primer, 15 pmol of a reverse primer, 1· PCR Gold buffer containing 2.5 mm MgCl2, 10 nmol each of dATP, dGTP, dCTP and dTTP, and U AmpliTaq Gold (Applied ... world wide web-based blastp search engine of GenBank (http:// www.ncbi.nlm.nih.gov/BLAST/) A further blastp search was conducted by an engine of the MEROPS database (http://merops.sanger.ac.uk)...
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Chapter 21 Network Layer: Address Mapping, Error Reporting, and Multicasting pdf
... Figure 21.5 Example 21.1, an ARP request and reply 21.9 Figure 21.6 Proxy ARP 21.10 Figure 21.7 BOOTP client and server on the same and different networks 21.11 Note DHCP provides static and dynamic ... format of ICMP messages 21.14 Note ICMP always reports error messages to the original source 21 .15 Figure 21.9 Error-reporting messages 21.16 Note Important points about ICMP error messages: ❏ ... total number of bytes in the ICMP packet (56 + 8) 21.24 Example 21.3 (continued) 21.25 Figure 21 .15 The traceroute program operation 21.26 Example 21.4 We use the traceroute program to find the...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... ExpressHyb solution (Clontech) Blots were washed thrice in · NaCl/Cit (1 · NaCl/Cit ¼ 0 .15 M NaCl, 0. 015 M sodium citrate), 0.05% (w/v) SDS at room temperature for 10 min, and twice in 0.1 · ... Phylogenetic analysis was performed by maximum parsimony using the PROTPARS program of the PHYLIP package version 3.57c [15] Support for the inferred clades was obtained by bootstrap analysis ... GLUT4 proteins was constructed by maximum parsimony (PROTPARS) and bootstrapped with 1000 replications using the PHYLIP package version 3.57c [15] As shown in Fig 3, gcGLUT was found to cluster in...
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Water Pollution: Impact of Pollutants and New Promising Techniques in Purification Process doc
... Radioactive waste: The problem and its management Current Science, 81(12): 153 4 -154 6 UN Works- The Global Water Crisis 2010 From< http www.un.org> (Retrieved October 29, 2011) 109 Gleick PH, Adams DB ... Water Treatment200 From (Retrieved March 20, 2009) Home Page of Mining and Water Pollution 2011 From (Retrieved ... From (Retrieved March 9, 2009) Official Home Page of UNICEF: Promotion of Household Water Treatment and Safe Storage in UNICEF Wa sh Programmes 011 From< http...
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