Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... impact on the gene expression patterns, when analyzing the efficacy and the genetic consequences of targeted gene therapy in vitro (and in vivo). Microarray Study Differential Gene Expression ... 2 min respectively. BeadArray gene expression analysis We used an integrated genomics profiling and computational biology based strategy to identify the key genes and gene clusters whose expression ... employed the Fmoc (9-fluorenyl-methyloxycarbonyl) methodology [37, 38] in a fully automated multiple synthesizer (Syro II from Multi Syntech Germany). As coupling agent 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethylu-roniumhexafluoro-phosphate...
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
... after its initial effect on protein kinases phosphorylation. The most strikingly and novel finding obtained hereby was the strong inhibition of the GPCR signaling pathway induced mainly by increased ... [36]. The kinase IKKα plays a key step in the activation of NFκB [37]. Our data demonstrated that ethanol inhibits the anti-apoptotic effect of the NFκB signaling pathway by reducing the phosphorylation ... concentration of ethanol in the intracellular signaling pathways activated by the Fas receptor pathway has not been documented. Therefore, the aim of the present study was to determine the effect of ethanol...
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Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt
... product, the c-mycbinding protein (MBP-1) lacking enzymatic activity.These divergent a-enolase gene products are interlinked: expression of the glycolytic enzyme a-enolase is upregu-lated by c-myc, ... polypeptides of the respira-tory chain). Mutations of the nDNA hitting genesinvolved in mitochondria biogenesis such as, for exam-ple, enzymes of the cardiolipin synthesizing pathway, the TCA cycle or ... control on glycogensynthesis [189–191]. The enzyme becomes inactiveupon phosphorylation either by the cAMP-dependentprotein kinase A or by the insulin-dependent glycogenA. Herling et al. Tumor...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... transformed into RAS46 andrecombinant strains carrying the chromosomally insertedplasmid (by means of single homologous recombination)were identified by their resistance to tetracycline and theirinability ... Germany). In general, mutant strains of the streptomycin resistantK. pneumoniae UN4495 strain (RAS46) were constructedusing the allelic exchange system developed by Skorupskyand Taylor [27]. The ... protein–protein interaction [3,4]. For the diazotroph A. vinelandii, the formation of NifL–NifA com-plexes has been demonstrated recently by in vitro co-chromatography and by using the yeast...
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Báo cáo Y học: Neurotrophic signalling pathway triggered by prosaposin in PC12 cells occurs through lipid rafts doc
... protein (LRP-1) [8]. The presence of lipid-binding domains in prosaposinand saposins has been well demonstrated [9], and aKeywordslipid domains; PC12 cells; prosaposin; rafts;sphingosine kinaseCorrespondenceR. ... revealed by scanning confocal microscopy and linear sucrosegradient analysis. In these cells, prosaposin is able to induce extracellularsignal-regulated kinase phosphorylation, sphingosine kinase ... of prosaposin againstapoptosis was partially inhibited, indicating that the signalling pathways involved in cell death preventiontriggered by prosaposin occur through lipid rafts. In addition,...
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Báo cáo y học: "Users' guides to the medical literature: how to use an article about mortality in a humanitarian emergency"
... in mind when reading amortality survey to determine the validity of the study and the applicability of the findings to theirsettings.Public health scenarioYou are a physician working for an international ... mortality isoccurring, by comparing the observed mortality rate withthe best available estimate of the baseline mortality rate inthe pre-war period, either by subtraction or by modellinga relative ... corroborate these findings from local independentsources?2. Using the guideReturning to our opening public health scenario, howwell did the study assessing nation-wide mortality in theDRC achieve the...
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
... Biol Ther 2003; 2: 491-9. 16. Huang S, Shao G, Liu L. The PR domain of the Rb-binding zinc finger protein RIZ1 is a protein binding interface and is related to the SET domain functioning in ... domain of human retinoblastoma pro-tein-binding zinc finger protein 1 (RIZ1). Int J Mol Sci 2008; 9: 943-50. 21. Chen L, Ling B, Alcorn J, Yang J. Analysis of the expression of human N-myristoyltransferase ... domain. The PR domain expression was examined by Western blot after 24 hr of transfection using β-actin as a loading control. As shown in Fig. 3C, the PR domain was expressed in the...
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Báo cáo y học: " Experimental ablation of the pancreas with high intensity focused ultrasound (HIFU) in a porcine model"
... parameters In the present study, the amylase levels in the serum and urine were increased in the first 3 days and the first 5 days after HIFU ablation, respectively. But no significant ... efficacy and safety. To evaluate the rela-tionship between HIFU energy and pancreas histol-ogy, a preclinical in vivo study was conducted in swine demons t rat ing the feasibility ... HIFU tu-mor therapy system (Model J C t y p e , Chongqing HaifuTech Co., Ltd, Chongqing City, China). The in- strument was composed of three parts: a firing system located in a degassed...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... Devine et al intravenously injected autologous and allogeneic MSC into baboons, and no toxic reactions were observed during 1-year follow-up [41]. Another intravenously injected allogeneic ... imaging system is a real-time imaging system which can be used to ob-serve the distribution of cells in living animals in a real time pattern. In the imaging system, ... gradually decreased, and nearly no GFP fluorescence was observed in nude mice at 96 h after transplantation. These findings in- dicate that intravenously injected MSC could migrate into the...
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Báo cáo y học: "Clinical Strategy for the Management of Solid Pseudopapillary Tumor of the Pancreas: Aggressive or Less"
... predominant presenting complaint was abdominal pain (8/18). The rest of the patients were asymptomatic and tumors were de-tected incidentally during routine physical examina-tion. All these patients ... (SPN), the experience with the management of disseminated disease is very limited in the literature6, therefore, attention to the therapy strategy of this neoplasm in the future may ... might in turn result in the uncertainty of the strategy for its treatment. While further delineati n g the clinicopathological and radiological features, and summarizing the surgical...
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