... labelling of proteins in the RTS 500 System. In Cell- Free Translation Systems (Spirin, A.S., ed), pp. 219–225.Springer, Berlin.3040. Kigawa, T., Muto, Y. & Yokoyama, S. (1995) Cell- free synthesisand ... analysed the effect of lipids on the cell- free GFP expression. L-a-phosphatidylcholine (LPC), 1,2-dimyris-toyl-sn-glycero-3-phosphocholine (DMPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine ... toxicity of the overproduced protein uponinsertion into the cytoplasmic membrane, poor growth of overexpressing strains and the proteolytic degradation of the proteins, could easily be eliminated...