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Molecular Farming of Antibodies in Plants

Molecular Farming of Antibodies in Plants

Molecular Farming of Antibodies in Plants

... 3 Molecular Farming of Antibodies in Plants 37 rearrangements of the germ-line immunoglobulin genes. This part of the molecule is responsible for antigen recognition and binding. The remainder ... chains are shown in gray and light chains in pink.Solidblack lines indicate continuation of the polypeptide backbone (simple lines indicate the constant parts of the antibody, curly lines indicate ... glycan chain structure between rodent and human cells. For example, human antibodies contain only the 3 Molecular Farming of Antibodies in Plants 39 Minibody Fab fragment Single chain Fv fragment...
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Báo cáo y học:

Báo cáo y học: "Long Term Persistence of IgE Anti-Influenza Virus Antibodies in Pediatric and Adult Serum Post Vaccination with Influenza Virus Vaccine"

... One software BioRad, Hercules, CA). Cytokine determination Serum cytokines [Interleukin-2 (IL-2), Interferon gamma (IFN-γ), Interleukin-4 (IL-4), Interluekin-10 (IL-10)] were determined ... IgE specific Influenza virus antibodies were increased after influenza vaccination in mice [17]. Interestingly, low levels of total Influenza virus-specific antibody secreting cells ... history of infection or vaccination. 4. Cytokines in serum. Similar levels of IL-2, IFN-γ, IL-4, and IL-10 cy-tokines were detected in serum of vaccinated com-pared with non vaccinated...
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Báo cáo y học:

Báo cáo y học: "Advances in Molecular Diagnosis of HBV Infection and Drug Resistance"

... two include the substitution of methionine (M) by the amino acids isoleucine (I) or valine (V) in the YMDD motif (C domain) at position rtM204V/I. In the majority of cases, these mutations in ... yes intermediate low low yes Florescence nd intermediate intermediate no intermediate intermediate intermediate yes MALDI-TOF <5% intermediate intermediate No intermediate intermediate ... its role as a ‘baseline’ indicator of HBV infectivity, the measurement of viral load is an indispensable means for monitoring and confirming resistance to lamivudine therapy. Indeed, resistance...
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Molecular Biology of Secondary Metabolism - Case Study for Glycyrrhiza Plants

Molecular Biology of Secondary Metabolism - Case Study for Glycyrrhiza Plants

... neededto feed an increasing world population. In addition, it shows the contribution of thisstrategy to the development of plants with biofarming potential in the frame of a highdemand of natural ... thepossibility of intein-mediated protein trans-splicing for limiting the environmentalimpact of herbicide resistance, by separating parts of the gene in plastid and nuclearChapter 5 Molecular Biology of ... (2005). Intein-mediated protein trans-splicing andtransgene containment in plastids. Trends Biotechnol. 23: 217–220.Kim, S.A. and Guerinot, M.L. 2007. Mining iron: iron uptake and transport in plants. ...
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Regulating Phytonutrient Levels in Plants – Toward Modification of Plant Metabolism for Human Health

Regulating Phytonutrient Levels in Plants – Toward Modification of Plant Metabolism for Human Health

... othersbeing delphinidin, peonidin, pelargonidin, petunidin, and malvidin. Of the flavonols,quercetin, kaempferol, myricetin, and isorhamnetin are common in fruits, quercetinbeing the predominant ... modifying a biosynthetic pathway to increase amounts of desirable phytonutrients are available: (1) manipulation of pathway flux, includ-ing increasing, preventing, or redirecting flux into or within ... 31112.5.4 Metabolic Engineering of the Flavonoid BiosyntheticPathway in TomatoThere is a growing interest in producing food plants with increased amounts of flavonoids because of their potential...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... domain, atransmembrane domain and an intracellular tyrosinekinase domain. The binding of a ligand to the extracel-lular domain of the EGFR induces receptor dimeriza-tion, activation of the intracellular ... carcinomas; IAP, inhibitor of apoptosis protein; JNK, c-Jun NH2-terminal kinase; KIP, kinase inhibitor proteins; MEK,MAPK ⁄ ERK kinase; MKK, MAPK kinase; MKP-1, mitogen-activated protein kinase ... CIP1is involved in gefitinib-induced growthinhibition in HNSCC [58]. Another group of cell-cycleregulatory molecules – those of the INK4 family – hasalso been implicated in gefitinib-induced inhibition...
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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc

Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc

... same antigen–antibody interaction in the presence of increasing concentrations of NaCl. In both experiments, binding intensities are represented in RU,and each data point shows the mean value ... appearance of a number of novel antigen-bindingspecificities (Fig. 1B).Enhanced binding of low-pH buffer-exposed IVIgto recombinant human interferon-c (IFN-c) andother self-antigensInteractions of ... obtained after single injections of native and of low-pH buffer-exposed IVIg were com-pared. As shown in Fig. 3A, transient (5 min) exposure of IVIg to pH 2.8 buffer resulted in an increase in...
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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... becom-ing pupae in about a week after spinning (Fig. 1B).Isovaleric acid seems to be the cause of prepupallethality because injection of isovaleric acid intonormal spinning larva induces ... thesku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid catabolism in insects.AbbreviationsACAD, acyl-CoA ... subtracting thereduction rate of the enzyme-excluded solution from that of the enzyme-containing solution and was expressed as nmols of 2,6-dichloroindophenol reduced per mg of protein permin....
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Tài liệu Báo cáo khoa học: Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

Tài liệu Báo cáo khoa học: Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

... addition of CtCBM11. In general, a progressive line broadening of all thecellohexaose protons was observed during titration withincreasing amounts of protein, which can be understoodas a result of ... (Fig. 1III–V), which could indicate that the corre-sponding hydroxyl groups are involved in proteinbinding.The results for the linebroadening measurements of protons H1a in the alpha and beta configurations,aHa1 ... madamm software[22] that allows the introduction of a certain degree of protein flexibility in standard docking processes.The process tries to mimic a conformational bindingmodel, in which...
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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... chemokines stimu-lated mainly by in ammatory cytokines, including IL-1b, TNF-a andIFN-c. (B) Chemokines expressed in the joint recruit leukocytes intothe joints. In addition to functioning in ... examination of affected rheu-matoid joints reveals extensive RANTES ⁄ CCL5expression in the synovial lining and sublining layers[22]. The expression of RANTES ⁄ CCL5 in culturedFLS increases in both ... IP-10, interferon-c-inducible protein-10; MAPK,mitogen-activated protein kinase; MCP, monocyte chemoattractant protein; Mig, monokine induced by interferon-c; MIP, macrophage in ammatory protein;...
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