... sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182–Lys186, pass through the a2 vicinity of the other monomer. For the FeR dimer, the N-terminal ... Japan). Then,we digested the genomic DNA with PstI and KpnI, and fractionated the products on an agarose gel. The separatedfragments were ligated into the corresponding sites inpUC18, and the ... present the crystal structure ofDvMF flavoredoxin and discuss the key residues forligand binding and metal ion binding, based on the crystal structures.ResultsCloning and sequencing of the flavoredoxin...