... reproductioneg Candida, Cryptococcus RNA viruses: Influenza Nature andProperties of Micro-organismsLaura JV Piddock VirusesObligate intracellular parasitesUse host cell for nucleic acid and ... invasiveness of pathogenic bacteria by reducing opsonisation and protecting from phagocytosis. E.g. Streptococcus pneumoniae in RTI vaccination: pneumococcal; meningococcal A and C GLYCOCALYX ... conjugation (transfer of plasmid DNA) Prions Infectious protein No DNA or RNA Normal cellular form of protein vs. pathological form Pathological form found in neural tissue and tonsilsPrP-C...
... somewhat expand.The dosimetric propertiesof the powder were studied. In the TL glow curve of LiF:Mg,Cu,Na,Sipowder the main dosimetric peak appeared in the range of 227-247oC and the ratio of the ... powder werestudied. The main dosimetric peak of LiF:Mg,Cu,Na,Si appeared in the range of 227− 247oC and the ratio of the height of the main peak to that of the other small peaks in the LiF:Mg,Cu,Na,Siis ... with rate of 4 l/min. After annealing treatment, the samples were washed with HCl solution.VNU Journal of Science, Mathematics - Physics 23 (2007) 225-231 Preparation and characteristics of LiF:Mg,Cu,Na,Sithermoluminescent...
... concentration of the SnCl4 solution, and the films were annealed at different temperatures and for different period of time. Photoconductivity measurement In order to evaluate propertiesof obtained ... (RD) and the light resistance (RL) under the irradiation of 7W Hg lamp at the distance of 10cm were measured. The calculated ratio of RD/RL was considered as a photoconductivity of obtained ... results of measurements show that all values RL, RD, and RD/RL strongly depend on the temperature and time of annealing as presented in Fig. 4. P.V. Nho, T.K. Cuong / VNU Journal of Science,...
... Identi®cation of the sipV(ba) and sipW(ba) gene regions of B. amyloliquefaciens. (A1/B1) PCR reactions with genomic DNA of B. amy-loliquefaciens and the degenerative primers V1/V3 and V2/V3 or W1 and ... PstIandEcoRI in case of sipV or EcoRI-SacIincaseofsipW and ligated into corresponding sites of pUC18 DNA. The ligation mixes were used for PCR with oligo nucleotides Uni1 or Uni2 and pairs of ... 2002Identi®cation andpropertiesof type I-signal peptidases of Bacillus amyloliquefaciensHoang Ha Chu, Viet Hoang*, Peter Kreutzmann², Brigitte Hofemeister, Michael Melzer and JuÈ rgen HofemeisterInstitute...
... theKm and kcatvalues of E. coli Fre for riboflavin arereported as 2.5 lm and 52.4 s)1with NADPH as theelectron donor and 1.3 lm and 30.6 s)1with NADH[13]. For FMN, Km and kcatvalues of ... twofold axis associated with thedimer passes through the vicinity of the side chains of Pro13, Ile126, Gln133 and Ile163. The correspondingTable 1. Summary of x-ray data collection, phasing and ... the case of A. vinelandii ferric reductase, the enzymewas purified as a mixture of two proteins or a hetero-dimer with molecular masses of 44 600 and 69 000 Da[25]. Although none of the amino...
... decomposition of tetra-thionate was proposed by Steudel et al.[16],Hazeuet al.[17] and Meulenberg et al. [18] in experiments with intactcells of A. ferrooxidans and A. acidophilum.Propertiesofpurified ... mMNADP+(Sigma) and enzyme. The production of NADPH was determinedspectrophotometrically at 340 nm. One unit of Glc6Pdehydrogenase was defined as the amount of protein thatreduced 1 lmol of NADP+in ... enzyme may bedue to several reasons. Copper and several other divalentions stimulated the activity of the tetrathionate hydrolase and they and other cofactors may be present in the crudeenzyme...
... lombricine (30% of that of taurocyam-ine) and glycocyamine (7% of that of taurocyamine). Neither TK catalyzedthe phosphorylation of creatine. Comparison of the deduced amino acidsequences of mitochondrial ... 3381–3385.Cytoplasmic and mitochondrial taurocyamine kinases K. Uda et al.3530 FEBS Journal 272 (2005) 3521–3530 ª 2005 FEBSOrigin andpropertiesof cytoplasmic and mitochondrialisoforms of taurocyamine ... correlations of this relaxed sub-strate specificity and the possibility of cytoplasmic and mitochondrial isoforms of TK, we have amplified twocDNAs coding for Arenicola brasiliensis TKs and cloned...
... mixture of 0.05MNaPibuffer, pH 3, and acetonitrile (6 : 1, v/v);separation was performed isocratically, at a flow rate of 1mLÆmin)1, and the volume of injected samples was 10 lL.The amounts of ... consistsactually of a unique enzymatic protein.Molecular mass and pI determination of F 4¢-OMT. Themolecular mass of the pure enzyme was calculated boththrough gel-filtration and PAGE (Fig. 7) ... the method of Baayen and Elgersma [13], with a 500-lLdropofFod conidialsuspension at a concentration of 9 · 106conidiaÆmL)1;20additional plants, inoculated with a 500-lL drop of doubledistilled...
... lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an[a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rabproteins, ... GST±Rab31 and Fig. 7. Puri®cation andproperties o f wild-type and mutant Rab31 and Rab32 expressed a s GST±fusion proteins. Samples of platelet particu-late fraction protein andof puri®ed GST and ... expression andpropertiesof Rab31 and Rab32New blood platelet Rab proteinsXiankun Bao1, Andrea E. Faris2, Elliott K. Jang1 and Richard J. Haslam1,2Departments of 1Pathology and Molecular...
... by MALDI-TOF mass spectrometry to be 51.0 kDa and it had pI values of 3.6, 3.9 and 4.4. The molecular mass of wolffish kininogenwas 45.8 kDa and it had pI values of 4.1, 4.3, 4.35 and 4.4.Partial ... Kalkkinen11Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland,2Norwegian College of Fishery Science,University of Tromsø, Norway,3Institute of Medical Biology, ... present in the MALDI-TOF mass maps from the 60 kDa band were also present inthe mass map from the 78-kDa band, suggesting that the60-kDa band represents a fragment of the 78-kDa band.Similar related...
... been synthesized using a novel Fe3O4nanoparticles catalyst.The lengths of SiOx nanowires are in the range of several tens to hundreds of micrometers, and the diameters of nanowires are 20–80 ... Preparationand photoluminescence of high density SiOx nanowires with Fe3O4nanoparticles catalystX.J. Wanga,⁎, B. Dongb, Z. ZhouaaSchool of Electronics Engineering and Computer ... assays, chemical sensors, and superparamagnets [8,9]. Recently, efforts have been devoted to preparation of Fe3O4nanoparticles, nanowires and nanotubes. In2002, Sun and Zeng [10] prepared the...
... kDa band in T cells. ACb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells. (C) Lin-ear depiction of Ca1, Cb1 and ... p50csk[16] and Raf-1 [17] inthe early phase of the stimulatory process and regula-tion of interleukin-2 production through phosphoryla-tion of the nuclear factor of activated T cells [18] and nuclear ... Jahnsen2 and Bjørn Steen Ska˚lhegg11 Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway2 Department of Biochemistry, Institute of Basic...
... The effect of geometrical parameters and material propertiesof facesheets and core are studied on the face wrinkling of sandwich plates. Comparison of the present results with those of plate ... anisotropic propertiesof strength and stiffness of masonry in horizontal and vertical direction, tensile and shear stiffness of cracked masonry,• Concrete yielding in compression, opening of cracks ... tension,mechanism of opening and closing of cracks in concrete under dynamic load,tensile and shear stiffness of cracked concrete,• Strain rate effect of the material propertiesof masonry, reinforced...
... expression andpropertiesof the recombinant enzymeY. T. Pan1, J. D. Carroll2 and A. D. Elbein11Department of Biochemistry and Molecular Biology and 2Department of Microbiology and Immunology, ... the cloning and expression of the M. tuberculosis tps gene in E.coli,andtheproduction of active TPS in these cells. The propertiesof therecombinant enzyme have been determined, and are com-pared ... starting at position 397 of the M. tuber-culosis TPS, and another sequence of about 16 amino acidsstarting at position 421 of the M. tuberculosis TPS.Isolation and purification of recombinant TPSTo...