ercises in Functional Analysis
ercises in Functional Analysis
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... N-terminal region of DEC1, including thebHLH domain, interacted with the C-terminal r egion o fBMAL1 in a mammalian two-hybrid assay. Accordingly, aFig. 5. Analysis of DEC1 mutants for bindingto ... estern blot analysis (Fig. 5 ). These r esults indicate that the bHLHregion, including His57 and Arg65, is responsible for t heE-box binding.Determination of the region in BMAL1 for bindingto ... by DEC1 coincidedwith that for interaction with BMAL1 and binding to theE-boxes, indicating the importance of t his region in DEC1for its suppressive activity against CLOCK/BMAL1-induced transcription.Materials...
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Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out withmyc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... mutant proteins were alsoobserved in the cytoplasm, as seen for the wild-typeprotein. Similar staining patterns were observed in other human cell lines, including hepatoma Hep3Bcells and fibroblast ... colocalizedwith the GALE proteins, suggesting that thesemutant proteins indeed become incorporated intoaggresomes.GALE activity towards UDP-galactoseTo determine the functional consequences of...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
... alsoaffects the binding of galactose at some point in thereaction. Modest changes in Km,ATPare seen in H44Y andR68C. That H44 in uences the binding of both substratessuggests that the binding sites ... change in the enzyme, which creates a functional binding site for galactose. Identifying the natureof this change and the residues involved in transmittinginformation through the protein will ... causing mutations can be a valuable tool in helping to assign functional roles to motifs and regions ofproteins. Furthermore, biochemical analysis of mutant pro-teins can help in understanding...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
... terpenesare produced in resin ducts [2,3]. Their biosynthesis isinduced upon wounding [4–6], indicating their role in thedefence against bark beetle infestation. For angiospermsmany investigations ... afterdrying dissolved in 5 mMTris, pH 6.8, 0.2% (w/v) SDS and1% glycerol. Protein concentration was determined usingthe BCA Protein assay kit using BSA as protein standardreference, according ... to 200 °Cat5°CÆmin)1with He gasflow at 3 mLÆmin)1. The compounds of interest weretransferred from GC1 to GC2 from 6.6 min to 7.1 min(a-pinene) and 10.2 min to 10.4 min (b-pinene). The fusedsilica...
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Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt
... such as: DNA-binding ferritin-like protein, ferritins and bacterioferritinsPutative dpsA geneThe Dps protein family includes DNA-binding hemoproteins in several bacteriaP3Medium increasesco6501 ... shows anincreased level in the dmdR1 mutant, is a DNA-bindingprotein with domains typical of the ferritin superfamily.This protein might be involved in a cascade of iron regu-lation in response ... Name Size Homology RemarksP1Large increasesco4018 Phosphatidylethanolamine-binding protein533 nt17 926 DaPhosphatidylethanolamine-bindingproteins in other bacteriaGene located upstream...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... same singlebinding site, rather that I-AniI appears to contain twobinding sites.Keywords: Aspergillus nidulans; homing endonuclease;RNA binding protein; DNA sliding; RNA splicing.Group I ... itsbinding affinity is not altered by the presence of DNA in theDNA binding site.Interestingly, when 3 nMRNA and 10 nMDNA wereadded simultaneously to a limiting amount of protein in TK8 ... pre-existing nucleic acid (DNA)binding site to function as an RNA binding protein [23].However, previous RNA deletion analysis argues that theprotein is recognizing considerably more than just a singleshort...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... °Cor22 °C), by using an alternative expression host{BL21(DE3)[pLysS]} or by including protease inhibi-tors in the solutions used during purification.Kinetic analysis of disease-causing mutationsThe ... protein and from each other. The main effectseen was in the turnover, kcat, and in the specificityconstant, kcat⁄ Km. Again, this contrasts with disease-causing mutations in galactokinase ... hasbeen solved, showing that a valine to methionine substi-tution at this position disrupts the packing close to thesugar-binding site, making it easier for the sugars tobind nonproductively...
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Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
... similarity to human uridine kinase, UMPK, andUDPK. Uridine kinases are downstream of UP in thepyrimidine salvage pathway. Homology searchesrevealed that several uridine kinases exist in the C. ele-gans ... protein (UPP-1) exhibited both uridine and thymidine phos-phorylase activity in vitro. Knockdown of other pyrimidine biosynthesisenzyme homologs, such as uridine monophosphate kinase and uridinemonophosphate ... human pyrimidine biosynthesis enzymes,including uridine kinase, UMPK, and UMPS, in C. elegans to test their roles in 5-FU function andwere able to identify homologs for most of them in C. elegans.To...
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Từ khóa: louis nirenberg topics in nonlinear functional analysissome topics in nonlinear functional analysistopics in nonlinear functional analysis nirenbergfunctional analysis of the cervicalfunctional analysis of intergenicproblems in toxin analysisuse of dummy variables in seasonal analysisBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDETrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roTranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ