louis nirenberg topics in nonlinear functional analysis
Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
Ngày tải lên :
19/02/2014, 16:20
... N-terminal region of DEC1, including the
bHLH domain, interacted with the C-terminal r egion o f
BMAL1 in a mammalian two-hybrid assay. Accordingly, a
Fig. 5. Analysis of DEC1 mutants for binding
to ... by DEC1 coincided
with that for interaction with BMAL1 and binding to the
E-boxes, indicating the importance of t his region in DEC1
for its suppressive activity against CLOCK/BMAL1-
induced transcription.
Materials ... Arg65, is responsible for t he
E-box binding.
Determination of the region in BMAL1 for binding
to DEC1
To identify the region in BMAL1 required for the binding to
DEC1, w e constructed e xpression...
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Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
Ngày tải lên :
07/03/2014, 00:20
... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with
myc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... protein. Tubulin serves as a loading control. The relative amount
of mutant protein was calculated based on the ratio of the band intensities of myc-GALE to tubulin in the western blot and indicated ... measuring the
intensity of the individual GALE and tubulin bands in
the western blot using Scion Image (Scion Corporation,
Frederick, MD, USA).
Generation of stable cell lines and BrdU
incorporation...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
Ngày tải lên :
08/03/2014, 02:20
... also
affects the binding of galactose at some point in the
reaction. Modest changes in K
m,ATP
are seen in H44Y and
R68C. That H44 in uences the binding of both substrates
suggests that the binding sites ... change in the enzyme, which creates a
functional binding site for galactose. Identifying the nature
of this change and the residues involved in transmitting
information through the protein will ... homoserine kinase, proton transfer is unlikely to play a
major role in the rate-determining step of catalysis.
Several of the disease-causing mutations
are not soluble following induction in
E....
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
Ngày tải lên :
08/03/2014, 23:20
... terpenes
are produced in resin ducts [2,3]. Their biosynthesis is
induced upon wounding [4–6], indicating their role in the
defence against bark beetle infestation. For angiosperms
many investigations ... after
drying dissolved in 5 m
M
Tris, pH 6.8, 0.2% (w/v) SDS and
1% glycerol. Protein concentration was determined using
the BCA Protein assay kit using BSA as protein standard
reference, according ... at 3 mLặmin
)1
. The compounds of interest were
transferred from GC1 to GC2 from 6.6 min to 7.1 min
(a-pinene) and 10.2 min to 10.4 min (b-pinene). The fused
silica capillary column in GC2 (30%...
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Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt
Ngày tải lên :
16/03/2014, 18:20
... DNA-binding protein
of the ferritin family
563 nt
20 052 Da
Contains domains typical of the ferritin-like
superfamily, such as: DNA-binding ferritin-
like protein, ferritins and bacterioferritins
Putative ... shows an
increased level in the dmdR1 mutant, is a DNA-binding
protein with domains typical of the ferritin superfamily.
This protein might be involved in a cascade of iron regu-
lation in response ... available
regarding ferritin regulation in Streptomyces species.
In addition to the 11 proteins listed in Tables 1 and
2, minor changes in other proteins were observed.
These proteins may be involved in other...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
Ngày tải lên :
23/03/2014, 11:20
... °Cor
22 °C), by using an alternative expression host
{BL21(DE3)[pLysS]} or by including protease inhibi-
tors in the solutions used during purification.
Kinetic analysis of disease-causing mutations
The ... these
enzymes and inserted into the corresponding sites in the
expression vector pET-21d (Merck, Nottingham, UK). The
entire GALE coding sequence was verified by DNA sequen-
cing.
The recombinant plasmid ... stained with
Coomassie blue. C, control lanes containing 50 l
M GALE; P, GALE
digested with 90 n
M thermolysin for 30 min at 37 °C; U, GALE
digested with 90 n
M thermolysin for 30 min at 37 °C in...
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Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 01:20
... similarity to human uridine kinase, UMPK, and
UDPK. Uridine kinases are downstream of UP in the
pyrimidine salvage pathway. Homology searches
revealed that several uridine kinases exist in the C. ele-
gans ... is expressed in the hypo-
dermis and intestine, both UP ⁄ UMPK in the hypo-
dermis and OPRT ⁄ UMPK in the intestine are essential
for mediating 5-FU function in C. elegans. It is also
possible ... a
control.
In vitro enzymatic assays
To induce the MBP–UPP1 fusion protein, 0.3 lm IPTG
was added to the culture. Induced fusion proteins were
purified using amylose resin (New England Biolabs),
according...
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nonlinear and mixed integer optimization fundamentals and applications topics in chemical engineering
Ngày tải lên :
01/04/2014, 11:35
... and
there exist nonlinear terms
in the
objective
function
and
constraints, then (1.1)
is a
mixed-integer nonlinear programming
MINLP
problem.
The
fundamentals
of
MINLP optimization
... multipliers
in a
constrained nonlinear optimization problem have
a
similar inter-
pretation
to the
dual variables
or
shadow prices
in
linear programming.
To
provide such
an
interpretation,
... stationary points
of the
Lagrange
function
correspond
to the
minimum
of
(3.3).
Illustration 3.2.2 Consider
the
following
two-variable
constrained nonlinear programming prob-
lem in the
...
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Báo cáo hóa học: " Some new nonlinear integral inequalities and their applications in the qualitative analysis of differential equations" potx
Ngày tải lên :
21/06/2014, 01:20
... )and
t
0
1
(
1
(s))
ds =
, a(t), a(t) are defined as in Theorem 2.1, and a(0) >
t
0
>0. If x Î C(R
+
, R
+
) satisfies the following integral inequality containing multiple
integrals
φ(x(t)) ≤ a(t)+
α
1
(t)
0
f ... R
+
), and a, j, ψ , ω, a,
b, J, Y are defined as in Theorem 2.3. If u ẻ C(R
+
ì R
+
, R
+
) satisfies the following inte-
gral inequality containing multiple integrals
φ(u(x, y)) ≤a(x, y)+
β
(
y
)
0
α
(
x
)
0
[f ... R
+
), i =1,2,anda, j, ψ, ω, J, Y
are defined as in Theorem 2.3. If u ẻ C(R
+
ì R
+
, R
+
) satisfies the following integral
inequality containing multiple integrals
φ(u(x, y)) ≤ a(x, y)+
β
(
y
)
0
α
(
x
)
0
[f
1
(s,...
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Báo cáo toán học: "Some inequalities in functional analysis, combinatorics, and probability theory" pdf
Ngày tải lên :
08/08/2014, 12:22
... 11T99
Abstract
The main purpose of this paper is to show that many inequalities in functional
analysis, probability theory and combinatorics are immediate corollaries of the best
approximation theorem in inner ... of combinatorics 17 (2010), #R58 4
Proof. Since the total number s of (n − 1)-dimensional hyperplanes passing through the
origin equals the total number of lines passing through the origin,
s ... electronic journal of combinatorics 17 (2010), #R58 3
The main purpose of this paper is to show that many inequalities in functional analysis,
probability theory and combinatorics are immediate...
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báo cáo khoa học: "Gene family structure, expression and functional analysis of HD-Zip III genes in angiosperm and gymnosperm forest trees" pps
Ngày tải lên :
11/08/2014, 11:21
... REV paralog potentially having been
lost during ancestral genomic rearrangements [42], and
resulting in a gain of function for the remaining REV
sequence in developing xylem and leaves. Ko et ... at 42
C in a solution containing 5ì SSC, 0.1% SDS, 0.02%
BSA (w/v), 0.01% herring sperm DNA (w/v), and 50%
formamide. The slides were then washed twice in 0.1ì
SSC, once in water, rinsed in 2-propanol, ... protein 0.000 0.390 -0.803 0.446
CN520368 POPTR_0008s06940 E-41 **Cys-3-His zinc finger protein 4.086 -0.332 -0.044 * 0.619
CN520805 POPTR_0009s02400 E-93 *leucine rich protein **Brassinosteroid insensitve
1-associated...
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báo cáo khoa học: " Functional analysis of B and C class floral organ genes in spinach demonstrates their role in sexual dimorphism" pdf
Ngày tải lên :
12/08/2014, 03:21
... -30 in the 5'UTR,
(ACAAAAAAGG) position 1083 in the second intron, and
(TCAAAAAAGG) position 3052 in intron 5. We detected a
single potential LEAFY binding site (CCATTGT) in the
second intron ... among the six individuals sequenced, including
all coding and non-coding regions. To determine if there
was any variation that was obscured in a heterozygous state
in the direct sequencing, we cloned ... (TCAT-
ATTTGG) is located in the second intron at position 785.
Similarly. LEAFY binding sites have been determined in
intronic regions in Arabidopsis B class genes [34,35]. The
spinach SpAP3 sequences...
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