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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

Báo cáo y học:

Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

... protein. Most of the protein was expressed in inclusion body. The fusion protein in inclusion body was purified by using High-Affinity Nickel Iminodiacetic Acid Resin and refolded in urea gradient ... of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo Tongfeng Zhao1, Jing Lv1, Jiangpei Zhao2, Xiao Huang3, and Haijuan ... produce gAd-GLP-1-A fusion protein. The digested products were analyzed by SDS-PAGE and Western blot anal-ysis. Assay of glucose-lowering effect of gAd-GLP-1-A fusion protein Male KM mice...
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Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx

Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx

... Serotonin (5-hydroxytryptamine) interactswith N-acetylneuraminic acid, which is often contained in glycolipids and glycoproteins on the cell surface[24]. We examined the synergistic effects of ... (1986) Involvement of finger domain and kringle 2 domain of tissue-type plas-minogen activator in fibrin binding and stimulation of activity by fibrin. EMBO J 5, 3525–3530.37 Bakker AH, Weening-Verhoeff ... of MAP kinase signal-ing in the tPA-treated cells (data not shown). Manytypes of MMP, including matrilysin, MMP-3 and MMP-2/9, are efficiently activated by trypsin-likeserine proteinases [44–46]....
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 Báo cáo y học:

Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"

... layers of reticular subtype of oral lichen planus patient (streptavidin-biotin amplified system, x 400). Figure 2. Increased labelling of hMSH2 protein in basal and intermediate epithelial layers ... counterstained with Meyer’s haematoxylin and mounted in PermountTM. Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized ... expression of hMSH2 protein by immunohistochemistry in twenty-six cases of oral lichen planus. Clinically, 12 of them were categorized into reticular subtype and 14 were atrophic/erosive. Ten cases of...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

... classes.Role of Tyr183 in catalysis In previous crystallographic studies it was shown that thehydroxyl side chain of Tyr183 is within hydrogen bondingdistance of the N-acetyl carbonyl oxygen of the ... activity of the pure recombinant protein was80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attempts to further purify therecombinant hevamine on a Mono S c olumn, ... Activities wereexpressedinUÆmg protein )1, one unit being the decrease of 0.001 absorbance units per min at 600 nm.Chitinase assaysTo determine chitinase activity, two different assays wereused. The...
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf

Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf

... the combination of the isoforms is yet to be determined.We are now trying to express fusion proteins of A. acetabulum proteolipid subunits and green fluorescent protein in A. acetabulum and tobacco ... complementability of the group 2proteins for the yeast vma3-mutant. A comparison of thestructures, especially in the four hydrophobic domains(underlined sequences in Fig. 1A), showed that domains I and ... divergent in group 1 and 2 proteins. Theresults we obtained indicate that these two domains mayplay a critical role in complementability. The genesAACEVAPD1 and 3 were constructed in another yeastexpression...
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx

Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx

... PRISM(http://www.graphpad.com).Single turnover assay Single turnover assays of the individual components(10nmolofTomoH,20nmolofTomoCandTomoDsubunits) and of each of their possible combinations, wereperformed by adding ... mass of about 10, 38 and 57 kDa, theexpected molecular size of recombinant subunits B, E and A, respectively. The identity of the proteins was furtherchecked by N-terminal sequencing of the ... rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein- dye binding. Anal. Biochem. 72, 248.26. Xia, B., Pikus, J.D., Xia, W., McClay,...
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... acid synthesis. Indeed, synthesis of blood coagulationproteins in vertebrates and toxins in the cone snail may besecondary functions of this enzyme.ACKNOWLEDGEMENTSWe especially appreciate ... (1997) Theactive site region of the vitaminK-dependent carboxylase includesboth the amino-terminal hydrophobic and carboxy-terminalhydrophilic domains of the protein. FEBS Lett. 413,1.21. Bouchard, ... carboxylases. In contrast, the C-terminal 25% of the protein shows nohomology to any of the other vitamin K-dependentcarboxylases.Fig. 1. Alignment of the amino-acid sequence of the vitamin K-dependent...
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Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx

Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx

... after 40 cyclesconsisting of 1 min at 94 °C, 1 min at 44 °Cand1minat72 °C in a Hybaid thermocycler. Subcloning in pZERO(Invitrogen) using EcoRI and BamHI resulted in loss of apart of the amplified ... guaninenucleotide-binding proteins: the G proteins. G proteinsplay a central role in a wide variety of signal transductionpathways, mediating the perception of environmental cues in all higher eukaryotic ... phosphoinosi-tide turnover, and G12regulates Na+/K+exchanges [6].All G proteins consist of three subunits, a, b and c,withthe nucleotide-binding and hydrolyzing a subunit definingthe protein s...
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx

Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx

... the site of injectionInjection of microorganisms resulted in a dramatic decrease in numbers of both circulating and tissue in ltratinghaemocytes within 3 h of injection. To study haemocytebehaviour ... immunoreactivity observed at the site of microbial injection precluded both clarification of themechanisms of penaeidin release from haemocytes, and determination of any potential involvement of penaeidin in the ... degranulation of granule-containingpenaeidin, or any migration of granules towards the cellperiphery, in contrast with regulated exocytosis reported in Tachypleus [33]. Penaeidin containing haemocytes...
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Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx

Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx

... Kuhne,Pierre Flandin, Jean-Paul Giacobino and Patrick MuzzinDepartment of Medical Biochemistry, Faculty of Medicine, University of Geneva, SwitzerlandUncoupling protein- 3 (UCP3) is a mitochondrial inner-membrane ... specific and total levels of COX protein were not significantly different in IMF and SSmitochondria. The yield in mitochondria, which wasdetermined by comparing the level of COX protein in thesum of ... Expression of uncoupling protein- 3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscletypes and its modulation by fastingMaria Jimenez, Cedric Yvon, Lorenz Lehr, Bertrand Le´ger,...
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