... PRISM(http://www.graphpad.com).Single turnover assay Single turnover assays of the individual components(10nmolofTomoH,20nmolofTomoCandTomoDsubunits) and of each of their possible combinations, wereperformed by adding ... mass of about 10, 38 and 57 kDa, theexpected molecular size of recombinant subunits B, E and A, respectively. The identity of the proteins was furtherchecked by N-terminal sequencing of the ... rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein- dye binding. Anal. Biochem. 72, 248.26. Xia, B., Pikus, J.D., Xia, W., McClay,...