... of PCaP1 orthologous protein in crude membrane fractions with anti-PCaP1 Lanes and 6, A thaliana; lanes and 7, Raphanus sativus; lanes and 8, Brassica rapa; lanes and 9, B rapa var glabra; lanes ... that the N-terminal part with 27 residues has the ability to localize the protein to the plasma membrane, and that Gly2 is essential for plasma membrane localization In vitro myristoylation To ... lanes and 10, B oleracea var italica The amount of protein applied was lg for A thaliana and 40 lg for the other plants 43 kDa) and Brassica oleracea var italica (broccoli, 41 kDa) (Fig 1B) The...