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Methods in molecular biology vol 1545 exosomes and microvesicles methods and protocols

methods in molecular biology, v.303. nanobiotechnology protocols, 2005, p.242

methods in molecular biology, v.303. nanobiotechnology protocols, 2005, p.242

... bridging protein consisting of the immunoglobulin G (IgG)-binding β2 domain of streptococcal protein G modified by genetic fusion with the positively charged leucine zipper interaction domain (PG-zb), ... III Series: Methods in molecular biology (Clifton, N.J.) ; 303 TP248.25.N35N35 2005 660.6 dc22 2005000473 Preface Increasingly, researchers find themselves involved in discipline-spanning science ... protein avidin A genetically engineered maltose-binding protein appended with the charged leucine zipper (MBP-zb) was used as a purification tool in conjunction with both types of bridging proteins...
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Excluded volume effects in molecular biology and extracellular matrix biochemistry  biophysical considerations and molecular modeling

Excluded volume effects in molecular biology and extracellular matrix biochemistry biophysical considerations and molecular modeling

... Crowding influences Intra-cellular Trafficking 6.4 Macromolecular Crowding on Protein-folding and Stability 6.5 Macromolecular Crowding Effects on Protein-Aggregation 6.6 Macromolecular Crowding Effects ... frequently increasing the binding strength by at least an order of magnitude (Wilf & Minton, 1981) 6.4 Macromolecular Crowding on Protein-folding and Stability Crowding influences protein stability ... Acid Single Strands 83 10.6.2 Mixed MMC greatly enhances DNA-thermal Stabilizing Effects 84 10.6.3 A Combined in vitro -in silico Approach 86 10.6.4 Crowding Effects by Confinement 87 11 Macromolecular...
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Molecular biology of glutamate dehydrogenase and glutamine synthetase in two air breathing teleosts

Molecular biology of glutamate dehydrogenase and glutamine synthetase in two air breathing teleosts

... roles of glutamate dehydrogenase and glutamate in nitrogen metabolism………………………………………………… Functional roles of glutamine synthetase and glutamine in nitrogen metabolism…………………………………………………………… Air- breathing ... three isoforms of gs are expressed in the liver, intestine and brain of M albus Terrestrial exposure resulted in a significant downregulation of gs mRNA expression in the liver and intestine of M ... glutamine synthesis involves glutamate dehydrogenase (Gdh) and glutamine synthetase (Gs), this study was undertaken to examine the molecular biology of Gdh and Gs in M anguillicaudatus and M albus,...
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Báo cáo y học:

Báo cáo y học: "Advances in Molecular Diagnosis of HBV Infection and Drug Resistance"

... in the HBV genomic sequence in the course of treatment In addition, indirect measures of lamivudine resistance including determination of ALT values, and especially HBV DNA (viral load) assays ... within a broadening and evolving clinical context Moreover, it is increasingly apparent that clinically relevant prediction and monitoring of viral resistance may not only require the use of existing ... generally defined clinically by a rise in serum HBV DNA levels during antiviral therapy Although HBV DNA assays will eventually detect changes in viral load as a consequence of emerging resistance...
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Molecular biology of the lung and kidney of the african lungfish, protopterus annectens, during three phases of aestivation cystic fibrosis transmembrane conductance regulator, gulonolactone oxidase and p53

Molecular biology of the lung and kidney of the african lungfish, protopterus annectens, during three phases of aestivation cystic fibrosis transmembrane conductance regulator, gulonolactone oxidase and p53

... in the lung of the control fish were expressed in the lung of fish undergoing the maintenance and arousal phases of aestivation Some of these isoforms, are known to lead to cystic fibrosis lung ... gulonolactone oxidase (gulo) from the kidney of P annectens and to determine the mRNA and protein expression of gulo/Gulo in the kidney and other organs during three phases of aestivation The novel ... among lungfishes The third objective was to sequence the p53 from the lung of P annectens and to determine its mRNA expression levels in the lung and kidney during three phases of aestivation The...
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Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

... be involved in recognition of the methylated Lys4 amino group Multidomain organization of LSD1 and its enzymatic activity in the chromatin context Genes coding for LSD1 ⁄ K -demethylase proteins ... protein–protein and DNA–protein interactions [36] The name of this domain derives from its original identification in the proteins SWI3, Rsc8 and Moira, which are ATP-dependent chromatin remodeling ... during cell division, and LSD1 was shown to be essential in resetting this memory in the germline in order to ensure pluripotency [21] In parallel, a role for LSD1 in gene activation was originally...
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molecular methods in developmental biology

molecular methods in developmental biology

... embryos, in which maternal dorsal determinants have been inactivated or removed, have been an effective tool for analyzing From: Methods in Molecular Biology, Vol 127: Molecular Methods in Developmental ... From: Methods in Molecular Biology, Vol 127: Molecular Methods in Developmental Biology: Xenopus and Zebrafish Edited by: M Guille © Humana Press Inc., Totowa, NJ 29 30 Newman and Krieg Fig Developmental ... Green tein factors are to be used in the medium, bovine serum albumin (BSA, Sigma) should be added to 0.1% w/v to block nonspecific protein binding The agarose lining of dissection and incubation...
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