... Gene Analysis, Methods in Molecular Biology, vol. 31: (Harwood, A. J., ed.), Humana, Totowa, NJ, pp. 121–134.Southern Blot of Large DNA Fragments 319 2. All the restriction enzymes used for PFGE ... Southern Blot of Large DNA Fragments 317 5. Carefully remove the blotting papers. Mark the location of the wells and the orientationof ... situated on anothercommon fragment that is 220 kb in length.Southern Blot of Large DNA Fragments 315 15. Hybond™ N+ membrane (Amersham ).16. 5X Denhardt’s solution (Appligene).17. Dextran sulfate...