... of Akt phosphorylation, LY294002 (10 μM, LY) was pretreated and the level of FMRP was determined by Western blot. (D) After LYtreatment, cell viability was measured by MTT assay, inhibition of ... However, interestingly,the up- regulation of FMRP protein by etoposide wasalso completely blocked by pretreatment with LY294002(Figure 2C). This suggests that Akt phosphorylation i srequired ... C, Nakamoto M, Yao X, Chan CB, Yim SY, Ye K, Warren ST, Bassell GJ:Excess phosphoinositide 3-kinase subunit synthesis and activity as anovel therapeutic target in fragile X syndrome. J Neurosci...