... primarycultu re used to generate the cell line, and DNA from the cell line. Interestingly, 63 were confirmed a s mutationsonlyinthecellline(Ti/Tv=1.1;onefailedassayinpri-mary culture). Of the ... homopolymerrun of three or more bases, had a GC content of ≤ 60%,was a edit distance of at least four away from all othermembersofthesetof96,andscreenednegativewhencompared with other adaptor ... Regional Center of Excellence for Biodefense andEmerging Infectious Diseases at the University of Washington(3U54AI05714), the Ministry of Science and Technology of China, 863 program (2006AA02A301),...