Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
... 80- 110 million years ago [35]. The redundancy between these 14 gene pairs has therefore been maintained for more than 80 million years of evolution. We believe that itis extremely unlikely that ... commonancestor 80- 110 million years ago [35]. It is extremelyunlikely that the redundancy between these 14 genes has been maintained for more than 80 million years of evolutionmerely as a consequence of ... RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolutionJulia Tischler*, Ben Lehner*†, Nansheng...
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Báo cáo y học: "Genomic context analysis in Archaea suggests previously unrecognized links between DNA replication and translation" potx
... clusters maintained only by chance (genes not yet separated by recombination)disappear, on average, more rapidly in the course of evolution than those maintained by selection pressure. In that case,clusters ... oligonucleotide/oligosaccharide-binding(OB)-fold containing protein, a recently described newGTPase, DnaG) are encoded by genes that co-localize withDNA replication genes, suggesting that they may be involved in DNA ... (seebelow). Furthermore, we believe that the presence of DNAreplication genes in the vicinity of two of the genes encodingthe subunits of the initiation factor aIF-2 is noteworthy. In eukaryotes,...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
... responsible for dietary-restriction-induced longevity in C. elegans. e most likely pathway exploited by dietary restriction in C. elegans is the TOR pathway. e physiological role of TOR kinase ... with phenylalanine and tyrosine. A similar increase in branched-chain amino acids has been found in animals carrying mutations in components of AbstractThe nematode Caenorhabditis elegans ... loss of function extends adult life by two- to threefold. daf-2 and age-1 specify steps in the insulin/insulin-like signaling (IIS) pathway, thereby in uencing metabolism. IIS signaling inactivates...
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Báo cáo y học: "Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions" pptx
... have overlapping function during morphogenesis(Table 2), indicating that the functional relationship between this pair of genes has been selectively maintained for over 100 million years. This ... means of synthetic genetic analysisDouble RNAi by soaking worms in dsRNA for two genes at once is an inefficient means of synthetic genetic analysis. Percent embry-onic lethality measured by both ... even less in C. elegans, indicating a significant role of theregulatory network in genetic buffering [7,8]. By identifying gene disruptions that are viable in wild type but lethal in aspecific...
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Báo cáo y học: "Modeling non-uniformity in short-read rates in RNA-Seq data" ppt
... the four isoforms, with exons zoomed in. The tail of exon 1 of the first isoform is 6 bp less than that of the other three isoforms. The second isoform has 7 exons, while the third isoform misses ... Statistical inferences for isoform expression in RNA- Seq. Bioinformatics 2009, 25:1026-1032.14. Pan Q, Shai O, Lee LJ, Frey BJ, Blencowe BJ: Deep surveying of alternative splicing complexity in the ... quantitative inferences of RNA- Seq data.Since the average preferences can vary substantially in short pieces of sequences, the improvement can be signif-icant. We believe that all quantitative analysis...
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Báo cáo y học: " Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus" pot
... showsdecay rates determined for all expressed ncRNAs andasRNAs during the time course (excluding tRNAs andrRNAs). Interestingly, many of these RNAs displayedshort decay rates of less than a minute ... fitting method for determining RNA decay rates, we found a median half-life of 2.4 minutes and a median decay rate of 2.6 minutes for expressed genes - twofold faster than that reported for any ... (RNase P sRNA, rnpB)was determined by 2- [dCt(geneX)-dCt(rnpB)], summarized as 2-ddCt.cDNA synthesis, labeling and microarray hybridizationLabeling, hybridization, staining and scanning...
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Báo cáo y học: " Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIVmac239" potx
... replicationkinetics in CEMx174 lines or in rhesus PBMC. Yet, thepU∆14 mutant was found to reduce vRNA packaging byapproximately 40% perhaps indicating that the secondarystructure of this element may be ... demonstrated that HIV-2, employs a co-translational mechanism of RNA packaging whereby newly translated Gag polyproteininteracts with vRNA while in a ribosomal translation com-plex, thereby imparting ... µg/ml). Sampleswere incubated for 20 min at 37°C, in the presence of 50U of DNAse I, followed by two extractions first in phenol:chloroform: isoamyl alcohol, then chloroform. Viral RNA was then precipitated,...
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Báo cáo y học: " Facilitators and obstacles in pre-hospital medical response to earthquakes: a qualitative study"
... came in large numbers in response to thePakistan earthquake [28]. In spite of the long delay, the role of internationalvolunteer teams is important in helping the affectedcommunity. International ... military air forcewithin a few hours of locating the earthquake. Some of them were medical doctors, including surgeons andemergency medicine physicians. The airport was full of casualties needing ... priority for the Ministry of Health of Iran prior to the Bam earthquake, asexplained by de Ville [18]. The major problem was thelack of coordination between the organizations responsi-ble for...
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Báo cáo y học: "Emergency intraosseous access in a helicopter emergency medical service: a retrospective study"
... week to 78 years old. Nearlyone third of all insertions (n = 22) were made in patients younger than two years. Intraosseous use byage is presented in Table 1.Presenting diagnosisIntraosseous ... number of patientstreatedIO insertionrate%0-2 years 18 453 3.97%3-6 years 0 198 0.00%7-17 years 5 486 1.03%18-78 years 47 4979 0.94%IO - Intraosseous.Sunde et al. Scandinavian Journal of ... rates of 5 to 8% in its third andfourth years of service, and zero failure rates in the first,second and fifth years. Insertion failures were equallydistributed among the physicians involved.Insertion-related...
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Báo cáo y học: "Iraqi health system in kurdistan region: medical professionals’ perspectives on challenges and priorities for improvement"
... [http://www.who.int/healthsystems/EN_HSSkeycomponents .pdf] .16. Ministry of Health: Proceedings of the conference on reform of Iraq nationalhealth system: Baghdad Baghdad: Ministry of Health; 2008.17. Albert ... health system. Even though the role of private sector in delivering health services in IraqiKurdistan is increasingly growing, it was not included in this study. However, we think that this study ... experience in anumber of countries particularly in the Middle East andthe advocacy of WHO for its adoption [18]. The need for adopting health insurance system is partly related tothe increasing role...
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