... 74 2 .7. 2 Gradient Elution, 75 2 .7. 3 Peak Capacity and Two-dimensional Separation, 76 2 .7. 4 Peak Tracking, 77 2 .7. 5 Secondary Equilibria, 78 2 .7. 6 Column Switching, 79 2 .7. 7 Retention Predictions ... Separation, 70 2.6.2 Mass Overload: Effect of Sample Weight on Separation, 71 2.6.3 Avoiding Problems due to Too Large a Sample, 73 2 .7 RELATED TOPICS, 74 2 .7. 1 Column Equilibration, 74 2 .7. 2 Gradient ... (1952) 679 .29. L. S. Ettre, LCGC, 19 (2001) 120.30. J. C. Giddings, Dynamics of Chromatography. Principles and Theory, Dekker, NewYork, 1965.31. L. R. Snyder, J. Chem. Ed., 74 (19 97) 37. 32....