... level only after 60 min (Fig. 1).Pyp1p and Pyp2p phosphatases appear to play a key role in the dephosphorylation of the osmotic stress-activated Sty1p[20]. Interestingly, however, the Sty1p activation ... toestimate the levels of Sty1p phosphorylation in strain TS-2(wak1–, Sty1p-Ha6H) to determine the role of Wak1p in the cold-induced activation process. As shown in Fig. 2A, onlya slight increase in ... in the cold. Surprisingly, however, the cold sensitivephenotype in atf1–or pcr1–strains is not shared by mutantsdisrupted in either sty1+or wis1+(Fig. 5), indicating that the role of...