... Yamaguchi H, Sasaki K, Satomi Y, Shimbara T, Kageyama H, Mondal MS, Toshinai K, Date Y, Gonzalez LJ, Shioda S, Takao T, Nakazato M, Minamino N. Peptidomic identification and bio-logical validation of ... (Promega Corp Madison, WI), or using an MTT [ 3-( 4,5-dimethylthiazol-2-yl )-2 ,5-diphenyltetrazolium bromide] assay.[24] Statistical analysis Statistical analyses were performed using Sig-maStat (version ... the manufac-turer’s procedure (Clontech, CA). Briefly, mouse Vgf cDNA (Salton, unpublished data) was isolated via Xba I-Apa I restriction cleavage, and cloned into the NheI-ApaI sites of a pShuttle...