... 5 mm dithiothreitol), incubated with or without3mm of the respective nucleotide or phosphate compound for 2 h at 4 °C, and stored for at least 2 weeks at )80 °Cbefore use for kinetic and molecular ... tetrameric and dimericforms in Figs 1 and 2 exhibited low or high catalyticefficiency. Therefore, the various incubated hTK1forms from Figs 1 and 2 were analyzed for theirkinetic behavior with dThd, ... form is more openthan hTK1. Therefore, at present, it appears that theadenosine group is very loosely bound to hTK1.In the present work, the part of the phosphatedonor that is necessary for...