... withrabbit anti-IDO polyclonal antibody (Transgenic Inc, Kobe,Japan), followed by Phycoerythrin-conjugated goat anti-rabbitimmunoglobulin, and then subjected to flow cytometric analy-sis (FACSCalibur; ... incubation.Reverse transcription–polymerase chain reaction analysis of indoleamine 2,3-dioxygenase and Foxp3 expressionTotal RNA (2 μg) was reverse transcribed into cDNA using a transcription kit (TaqMan Reverse ... moderate edema and erythema from the ankle to the tarsalbone, and 4 = edema and erythema from the ankle to the entireleg. The sum of the values from three legs, excluding the hindleg into...