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Báo cáo y học: "Intrapleural instillation of autologous blood for persistent air leak in spontaneous pneumothoraxis it as effective as it is safe" pps

Báo cáo y học:

Báo cáo y học: "Intrapleural instillation of autologous blood for persistent air leak in spontaneous pneumothoraxis it as effective as it is safe" pps

... STU D Y PRO T O C O L Open AccessIntrapleural instillation of autologous blood for persistent air leak in spontaneous pneumothorax- is it as effective as it is safe?Dimos Karangelis*, Georgios ... patients of high morbidity andmortality surgical risks who are ineligible for surgery.Conclusively blood pleurodesis is an “only-win” method as there are minimal complications related to it, whilethe ... pleurodesis with autologous blood in patients with persistent air leak. Ann Thorac Surg2000, 70:270-272.10. Shackcloth MJ, Poullis M, Jackson M, et al: Intrapleural instillation of autologous blood in...
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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx

... Japan.5Laboratory for Systems Biology and Medicine, RCAST, The University of Tokyo, Tokyo, Japan.6Dept. of Biophysics and Biochemistry, Osaka University,Graduate School of Medicine, Suita, Japan.7Medical ... immunohistochemically except for 2 cases w ithfaintly positive for Syn but histopathological differentiationfrom SC, AC and SCLC was required for its definite diag-nosis. The patient profiles including the ... Ostmark B, Benson NA: Heterogeneity of aldehydedehydrogenase expression in lung cancer cell lines is revealed byAldefluor flow cytometry-based assay. Cytometry B Clin Cytom 2007,72:281-9.24. Patel...
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Báo cáo y học: "Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection" potx

... primarygoal of ACS treatment is alleviation of OA symptoms,one of the mechanisms may be enhancement of cartilageintegrity through the inhibition of inflammatory activity, in particular with ... bioactivity of most cytokines, while theirimmunoreactivity as determined by ELISA is stillretained [38]. In particular Il-10, one of the anti-inflam-matory cytokines upregulated in ACS, has been ... showing that this concentration was capable of inhibiting the activity of 40 ng/ml of TNFα [31].35S incorporation was measured by means of a four-hour incubation with 35SO42-, on Day 4 for...
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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"

... both in total number and in maturity, within the thymus. The increase in B cell numbers in the thymus with age seems too small to account for the greatly increased quantities of immunoglobulin ... 3. Protein levels of immunoglobulin increase within the thymocytes with age. Western blot analysis of IgM heavy chain protein levels with successive age. This image is representative of 3 experiments ... plasma cells were not contributing to the increased production of immunoglobu-lin with age, as flow cytometric analysis for B220, CD38 and CD138 on the thymocytes as well as RT-PCR analysis for...
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Tài liệu Báo cáo Y học: Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation pdf

Tài liệu Báo cáo Y học: Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation pdf

... Enthalpy and heat capacity changes for formation of anoligomeric DNA duplex: interpretation in terms of coupled pro-cesses of formation and association of single-stranded helices.Biochemistry 38, ... Moreover, the development of DNA chips for rapidly screening and sequencingunknown DNAs mainly relies on the ability to predict thethermodynamic stability of the complexes formed by theoligonucleotide ... theenthalpy–entropy compensation is significant and a largeincrease in the enthalpy change is necessarily accompaniedby the large increase in the entropy change. Compared withthe compensation of the...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot

Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot

... theNetherlands) in order to obtain a recombinant proteincontaining the C-myc epitope followed by six histidines atthe C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing ... were obtained with CCR5-myc-his proteinFig. 3. CCR5-myc-his purification. CCR5-myc-his was purified byimmobilized metal ion affinity (A) or immunoaffinity chromatographyusing anti-histidine Igs (B). ... protein, whereas 36% of the total radioactivity wasassociated with CCR5-myc-his after purification on anantihistidine column.Reconstitution of CCR5-myc-hisA limited number of protocols for...
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Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

... machinery towards the synthesis of s omeenzymes involved in glycolysis or sugar-phosphatemetabolism [54].HydA belongs to a new class of Fe-hydrogenasesHydA of C. reinhardtii, the first isolated ... vol. of fresh TAP medium and th ealgae were a naerobically adapted b y flushing the s olutionwithargoninthedark.Hydrogen evolution assayHydrogenase activity of C. reinhardtii was determined in ... fundamental data for studying thehydrogen metabolism in photosynthetic eukaryotes. Onthe b asis of this research, we have r ecently published theisolation and characterization of the hydA gene fromthe...
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Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx

Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx

... Ca of Glu and itscarboxylate oxygen, Ca of Asp a nd its carboxylate oxygen,Ca of Arg and its side-chain amines or guanidinium, and Ca of Lys and its side-chain amine was determined bymeasuring ... heN-terminal amine of t he P1 residue with a c arboxylateoxygen of Asp273 (Asp366), the carbonyl oxygen of the P1residue with the side-chain amine of Lys262 (Lys354), andthe backbone amino group of the ... with13% of the w ild-type activity on Leu-G ly. This change in hydrolytic activity was correlated with a 2.9-fold change in the Km of the R431K His6–LAP-A (5.1 mM)relativetowild-type His6–LAP-A....
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Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf

Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf

... unchanged in contrast to the highly increased affinity for ATP [36].Similar findings were made by uncoupling E. coli cellsexpressing the rat AACs with CCCP. The affinity for ADP of both rat AACs was ... Germany). T7-RNA polymerase synthesis was induced by adding 1 mMIPTG. After shaking the culture for 15 min at 37 °C, 20 lLrifampicin (stock 20 mgÆmL)1, dissolved in methanol) wasadded to inhibit ... differenttype of nucleotide transporter that seems to be specialized in ATP uptake [1–3]. In many multicellular animals and plants as well as in yeast (Saccharomyces cerevisiae),twotofourAACisoformshave...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

... of cofactor for TDP enzymaticactivity was examined by determining the specific activity of TDP by following the cleavage of 1 mM of substrate by0.125 lM of enzyme in reaction buffer containing ... that this assay has a sensitivityconcentration as low as 15 nM, which is comparable withthe sensitivity of the gel-based assay [14].Kinetic parametersKm,kcatandVmaxdeterminedunder ... produced no increase in A415, confirming that thehydrolysis of the tyrosine-DNA phosphodiester bonddetected before was indeed produced by the activity of purified recombinant huTDPND39 enzymes.The...
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